Rapid removal of regulatory light chains from scallop heavy meromyosin

1985 ◽  
Vol 13 (5) ◽  
pp. 926-927
Author(s):  
ANDREW J. BENNETT ◽  
CLIVE R. BAGSHAW
Keyword(s):  
Light Chains ◽  
Heavy Meromyosin ◽  
Regulatory Light Chains ◽  
Rapid Removal

scholarly journals The mechanism of regulatory light chain dissociation from scallop myosin

1986 ◽  
Vol 233 (1) ◽  
pp. 179-186 ◽  
Author(s):  
A J Bennett ◽  
C R Bagshaw
Keyword(s):  
Light Chain ◽  
Structural Information ◽  
Regulatory Light Chain ◽  
Light Chains ◽  
Heavy Meromyosin ◽  
Regulatory Light Chains ◽  
First Order ◽  
High Affinity Site ◽  
Refractory State ◽  
Kinetics Of

The dissociation of the regulatory light chains from scallop myosin subfragments, on addition of EDTA, was investigated by using the fluorophore 8-anilinonaphthalene-1-sulphonate as a probe. The rate of this process (0.014 s-1) was partially limited by the rate of Mg2+ dissociation (0.058 s-1) from the non-specific high-affinity site. The dissociation of the regulatory light chain subfragment 1 was less extensive than from heavy meromyosin. Reassociation of the scallop regulatory light chain was induced on addition of Mg2+, but it appeared to be limited by a first-order step. The nature of this step was revealed by the kinetics of Mercenaria regulatory light chain association. Scallop heavy meromyosin, denuded of its regulatory light chains, exists in a refractory state, whose reversal to the nascent state limits the rate of light chain association (0.006 s-1). The formation of the refractory state is the driving force for the net dissociation of regulatory light chains from scallop heavy meromyosin. This mechanism is discussed with reference to existing structural information on light-chain-denuded myosin.

Expression of myosin regulatory light chains in rat brain: characterization of a novel isoform

1991 ◽  
Vol 10 (2) ◽  
pp. 97-105 ◽  
Author(s):  
Douglas L. Feinstein ◽  
Michele Durand ◽  
Robert J. Milner
Keyword(s):  
Rat Brain ◽  
Light Chains ◽  
Regulatory Light Chains

scholarly journals 3P172 Dissociation of regulatory light chains from oyvster myosin observed by atomic force microscopy

2005 ◽  
Vol 45 (supplement) ◽  
pp. S246
Author(s):  
M. Taniguchi ◽  
H. Oonishi ◽  
Y. Yazawa ◽  
T. Yamane
Keyword(s):  
Atomic Force Microscopy ◽  
Light Chains ◽  
Force Microscopy ◽  
Regulatory Light Chains ◽  
Atomic Force
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