Transport of the aromatic amino acids into isolated rat liver cells by a ‘T’ system

1985 ◽  
Vol 13 (4) ◽  
pp. 693-694
Author(s):  
MARK SALTER ◽  
RICHARD G. KNOWLES ◽  
CHRISTOPHER I. POGSON
Keyword(s):  
Amino Acids ◽  
Rat Liver ◽  
Aromatic Amino Acids ◽  
Liver Cells ◽  
Isolated Rat Liver ◽  
Rat Liver Cells ◽  
Isolated Rat ◽  
T System

scholarly journals Transport of the aromatic amino acids into isolated rat liver cells. Properties of uptake by two distinct systems

1986 ◽  
Vol 233 (2) ◽  
pp. 499-506 ◽  
Author(s):  
M Salter ◽  
R G Knowles ◽  
C I Pogson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Rat Liver ◽  
Transport System ◽  
Aromatic Amino Acids ◽  
Liver Cells ◽  
Transport Systems ◽  
Isolated Rat Liver ◽  
Rat Liver Cells ◽  
Isolated Rat

The transport of the aromatic amino acids into isolated rat liver cells was studied. There was a rapid and substantial binding of the aromatic amino acids, L-alanine and L-leucine to the plasma membrane. This has important consequences for the determination of rates of transport and intracellular concentrations of the amino acids. Inhibition studies with a variety of substrates of various transport systems gave results consistent with aromatic amino acid transport being catalysed by two systems: a 2-aminobicyclo(2,2,1)heptane-2-carboxylic acid (BCH)-insensitive aromatic D- and L-amino acid-specific system, and the L-type system (BCH-sensitive). The BCH-insensitive component of transport was Na+-independent and facilitated non-concentrative transport of the aromatic amino acids; it was unaffected by culture of liver cells for 24 h, by 48 h starvation, dexamethasone phosphate or glucagon. Kinetic properties of the BCH-inhibitable component were similar to those previously reported for the L2-system in liver cells. The BCH-insensitive component was a comparatively low-Km low-Vmax. transport system that we suggest is similar to the T-transport system previously seen only in human red blood cells. The results are discussed with reference to the importance of the T- and L-systems in the control of aromatic L-amino acid degradation in the liver.

scholarly journals The effects L-leucine on the synthesis of urea, glutamate and glutamine by isolated rat liver cells

1975 ◽  
Vol 146 (2) ◽  
pp. 457-464 ◽  
Author(s):  
J M Mourão ◽  
J D McGivan ◽  
J B Chappell
Keyword(s):  
Amino Acids ◽  
Nitrogen Source ◽  
Glutamate Dehydrogenase ◽  
Rat Liver ◽  
Liver Cells ◽  
Carbamoyl Phosphate ◽  
Isolated Cells ◽  
Isolated Rat Liver ◽  
Rat Liver Cells ◽  
Isolated Rat

With either alanine or a mixture of 15 different amino acids as nitrogen source, the addition of L-leucine inhibited the synthesis of urea by isolated rat liver cells. With alanine present leucine promoted the production of glutamate and glutamine. Comparison of effects of leucine on soluble glutamate dehydrogenase, mitochondria and isolated cells supports the postulate that leucine exerts its effect through activation of glutamate dehydrogenase. It is suggested that this latter enzyme may not be as important for the production of NH3 for carbamoyl phosphate synthesis as has been considered hitherto.

scholarly journals The transport of branched-chain amino acids into isolated rat liver cells

FEBS Letters ◽  
1977 ◽  
Vol 80 (2) ◽  
pp. 380-384 ◽  
Author(s):  
J.D. McGivan ◽  
N.M. Bradford ◽  
J. Mendes-Mourrão
Keyword(s):  
Amino Acids ◽  
Rat Liver ◽  
Branched Chain Amino Acids ◽  
Liver Cells ◽  
Isolated Rat Liver ◽  
Rat Liver Cells ◽  
Branched Chain ◽  
Isolated Rat

Gene expression of syndecans and betaglycan in isolated rat liver cells

1996 ◽  
Vol 285 (1) ◽  
pp. 11-16 ◽  
Author(s):  
O. H. Weiner ◽  
M. Zoremba ◽  
A. M. Gressner
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Liver Cells ◽  
Isolated Rat Liver ◽  
Rat Liver Cells ◽  
Isolated Rat
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