The guinea-pig enterocyte as a model for the investigation of glycoprotein biosynthesis: Differential expression of brush-border glycoproteins along the small intestine

1983 ◽  
Vol 11 (2) ◽  
pp. 170-171
Author(s):  
JOANNE C. WALL ◽  
JOHN HERMON-TAYLOR ◽  
DAVID S. BAILEY
Keyword(s):  
Small Intestine ◽  
Guinea Pig ◽  
Differential Expression ◽  
Brush Border ◽  
Glycoprotein Biosynthesis

scholarly journals Glucose metabolism in the mucosa of the small intestine. A study of hexokinase activity

1968 ◽  
Vol 109 (1) ◽  
pp. 35-42 ◽  
Author(s):  
L M Srivastava ◽  
P. Shakespeare ◽  
G. Hübscher
Keyword(s):  
Small Intestine ◽  
Lactate Dehydrogenase ◽  
Glucose Metabolism ◽  
Guinea Pig ◽  
Intestinal Mucosa ◽  
Brush Border ◽  
Deae Cellulose ◽  
Mitochondrial Fraction ◽  
Type Ii ◽  
Hexokinase Activity

1. The intracellular distribution of hexokinase activity was studied in the mucosa of rat and guinea-pig small intestine. In the rat 60% and in the guinea pig 45% of the hexokinase activity of homogenates were recovered in a total particulate fraction that contained only 5–17% of the homogenate activity of hexose phosphate isomerase, pyruvate kinase, lactate dehydrogenase and overall glycolysis (formation of lactate from glucose). 2. Fractionation of homogenates from guineapig small intestine showed that the particulate hexokinase activity was chiefly in the mitochondrial fraction with a small proportion in the nuclei plus brush-border fraction. 3. After chromatography of the particle-free supernatants on DEAE-cellulose, hexokinase types I and II were determined quantitatively. No evidence was obtained for the presence of hexokinase type III or glucokinase. In the preparations from guinea pigs, hexokinase types I and II amounted to 69% and 31% respectively of the eluted activity; the corresponding values for preparations from rats were 5·8% and 94·2%. 4. Total and specific hexokinase activities decreased significantly in homogenates and particle-free supernatants prepared from the intestinal mucosa of rats starved for 36hr. and increased again after re-feeding. The decrease in hexokinase activity in the particle-free supernatant from starved rats was chiefly due to a decrease in the type II enzyme.

scholarly journals Mammalian small intestinal phytase (EC 3.1.3.8)

1983 ◽  
Vol 50 (3) ◽  
pp. 673-678 ◽  
Author(s):  
John R. Cooper ◽  
Helen S. Gowing
Keyword(s):  
Small Intestine ◽  
Guinea Pig ◽  
Metal Ions ◽  
Brush Border ◽  
Small Intestinal ◽  
Competing Reactions

1. Phytase (EC 3.1.3.8) concentration has been measured in the small intestineof rat, rabbit, guinea-pig and hamster. Levels varied from 0·12 units (μg phosphorus released/min)/mg protein in the rat to 0·03 units/mg protein in the rabbit.2. The enzyme is localized in the brush border of the small intestine of the rat.3. It is suggested that the levels and location of phytase are an important factor inthe uptake of metals from metal–phytate complexes. Metal ions released in the immediate vicinity of the absorptive surface of the intestine could be absorbed before being rendered insoluble by competing reactions such as hydrolysis.

Immunochemical visualization of cyclic AMP in myenteric neurons of guinea-pig small intestine+

2001 ◽  
Vol 120 (5) ◽  
pp. A683-A683
Author(s):  
J GUZMAN ◽  
S SHARP ◽  
J YU ◽  
F MCMORRIS ◽  
A WIEMELT ◽  
...  
Keyword(s):  
Small Intestine ◽  
Cyclic Amp ◽  
Guinea Pig ◽  
Myenteric Neurons

Effects of stimulating protease-activated receptors on myenteric newrons in guinea-pig small intestine

2001 ◽  
Vol 120 (5) ◽  
pp. A114-A114
Author(s):  
C GAO ◽  
H HU ◽  
S LIU ◽  
N GAO ◽  
Y XIA ◽  
...  
Keyword(s):  
Small Intestine ◽  
Guinea Pig ◽  
Protease Activated Receptors

Hydrolysis and transglycosylation activities of glycosidases from small intestine brush-border membrane vesicles

2021 ◽  
Vol 139 ◽  
pp. 109940
Author(s):  
Lesbia Cristina Julio-Gonzalez ◽  
F. Javier Moreno ◽  
María Luisa Jimeno ◽  
Elisa G. Doyagüez ◽  
Agustín Olano ◽  
...  
Keyword(s):  
Small Intestine ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles
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