REGULATION OF ADIPOSE TISSUE LIPOLYSIS: HORMONAL EFFECTS ON PHOSPHORYLATION AND ACTIVITY OF HORMONE-SENSITIVE LIPASE IN INTACT RAT ADIPOCYTES

1981 ◽  
Vol 9 (2) ◽  
pp. 236P-236P
Author(s):  
Nils Östen Nilsson ◽  
Per Belfrage
Keyword(s):  
Adipose Tissue ◽  
Hormone Sensitive Lipase ◽  
Hormonal Effects ◽  
Rat Adipocytes ◽  
Hormone Sensitive ◽  
Adipose Tissue Lipolysis

scholarly journals Regulation of adipose tissue lipolysis: phosphorylation of hormone-sensitive lipase in intact rat adipocytes

FEBS Letters ◽  
1980 ◽  
Vol 111 (1) ◽  
pp. 120-124 ◽  
Author(s):  
Per Belfrage ◽  
Gudrun Fredrikson ◽  
Nils Östen Nilsson ◽  
Peter Strålfors
Keyword(s):  
Adipose Tissue ◽  
Hormone Sensitive Lipase ◽  
Rat Adipocytes ◽  
Hormone Sensitive ◽  
Adipose Tissue Lipolysis

scholarly journals The contribution of hormone sensitive lipase to adipose tissue lipolysis and its regulation by insulin in periparturient dairy cows

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Jenne De Koster ◽  
Rahul K. Nelli ◽  
Clarissa Strieder-Barboza ◽  
Jonas de Souza ◽  
Adam L. Lock ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Dairy Cows ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive ◽  
Adipose Tissue Lipolysis

scholarly journals The presence and role of hormone-sensitive lipase in heart muscle

1989 ◽  
Vol 258 (1) ◽  
pp. 67-72 ◽  
Author(s):  
C A Small ◽  
A J Garton ◽  
S J Yeaman
Keyword(s):  
Adipose Tissue ◽  
Heart Muscle ◽  
Lipolytic Activity ◽  
Hormone Sensitive Lipase ◽  
Energy Store ◽  
Dependent Protein ◽  
Hormone Sensitive ◽  
Rat Myocytes ◽  
Adipose Tissue Lipolysis

Hormone-sensitive lipase (HSL) catalyses the initial, rate-limiting, reaction in adipose-tissue lipolysis. Hormone-stimulated lipolytic activity has also been observed in the heart, where endogenous triacylglycerol is the major energy store. However, the identity of the intracellular lipase responsible has yet to be established. We have partially purified a neutral lipase from bovine heart muscle and compared its properties with those of HSL from bovine adipose tissue. The heart lipase has the same subunit Mr as HSL, is immunoprecipitated by antiserum raised against purified HSL and is phosphorylated by cyclic AMP-dependent protein kinase, apparently at the same site as HSL (as judged by h.p.l.c. of tryptic phosphopeptides). Phosphorylation of the heart lipase was found to result in increased enzyme activity, demonstrating the lipase's potential to respond to hormonal stimuli. The heart lipase was shown to be present in myocytes by its immunoprecipitation from homogenates of rat myocytes by anti-HSL antiserum. These findings are consistent with the conclusion that HSL is responsible for intracellular lipolysis in heart.

scholarly journals The protein phosphatases responsible for dephosphorylation of hormone-sensitive lipase in isolated rat adipocytes

1993 ◽  
Vol 295 (2) ◽  
pp. 531-535 ◽  
Author(s):  
S L Wood ◽  
N Emmison ◽  
A C Borthwick ◽  
S J Yeaman
Keyword(s):  
Adipose Tissue ◽  
Phosphatase Activity ◽  
Protein Phosphatases ◽  
Total Activity ◽  
Hormone Sensitive Lipase ◽  
Rat Adipocytes ◽  
Hormone Sensitive ◽  
Total Phosphatase Activity ◽  
Isolated Rat

The levels of the cytosolic serine/threonine protein phosphatases (PP) in rat adipocyte extracts have been determined, by using both reference substrates and hormone-sensitive lipase (HSL) as substrates. Adipocytes contain significant levels of both PP1 and 2A (1.6 and 2.0 m-units/ml of packed cells respectively), with lower levels of PP2C and virtually no PP2B activity. PP2A and 2C exhibit similar degrees of activity against HSL phosphorylated at site 1, together accounting for 92% of the total. In contrast, site 2 is dephosphorylated predominantly by PP2A (over 50% of total activity), whereas PP1 and PP2C contribute approx. 20% and 30% respectively to the total phosphatase activity against that site. Total phosphatase activity in the adipocyte extracts was 2-3-fold higher against site 2 than against site 1. The possible significance of these findings to the regulation of HSL activity in adipose tissue in vivo is discussed.

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