TRYPTIC DIGESTION AS A PROBE TO STUDY CHROMATIN SUPERSTRUCTURE

1981 ◽  
Vol 9 (2) ◽  
pp. 124P-124P
Author(s):  
R. M. Nieto-Sandoval ◽  
L. Franco
FEBS Letters ◽  
1983 ◽  
Vol 157 (1) ◽  
pp. 111-114 ◽  
Author(s):  
G.T.P. Saccone ◽  
J.D. Skinner ◽  
L.A. Burgoyne

1967 ◽  
Vol 242 (2) ◽  
pp. 265-270
Author(s):  
Bryce V. Plapp ◽  
M.A. Raftery ◽  
R. David Cole
Keyword(s):  

1939 ◽  
Vol 39 (3) ◽  
pp. 268-270 ◽  
Author(s):  
C. E. Coulthard

The British Standard Technique for Determining the Rideal-Walker Coefficient of Disinfectants (no. 541—1934), in laying down the procedure to be adopted with regard to the test typhoid bacillus culture, recommends that a fresh culture be obtained each month from the National Collection of Type Cultures. In all probability this is the best procedure; it does, however, sometimes happen that a test is required urgently when a fresh culture is not available. The following notes upon our own experience in maintaining cultures may therefore be of some interest. It is our practice to obtain fresh cultures whenever we are called upon to carry out other than routine tests unless a recent one has been obtained, and many of these cultures have now been running for a number of years. On the arrival of the culture from the N.C.T.C. it is subcultured on to an agar slope and “incubated” at room temperature in a dark cupboard. 2% agar is used in a broth prepared by the tryptic digestion of ox heart. Further subcultures are made at intervals of one month. It is our usual practice to start a series of broth cultures from such slopes not less than 14 days after sowing.


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