Phosphate uptake by proximal-tubule cells isolated from chick kidney

1981 ◽  
Vol 9 (5) ◽  
pp. 465-466 ◽  
Author(s):  
MICHAEL F. GRAHN ◽  
PETER J. BUTTERWORTH
Keyword(s):  
Proximal Tubule ◽  
Phosphate Uptake ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Chick Kidney

Effect of adenosine triphosphate on phosphate uptake in renal proximal tubule cells: Involvement of PKC and p38 MAPK

2005 ◽  
Vol 205 (1) ◽  
pp. 68-76 ◽  
Author(s):  
Yun Jung Lee ◽  
Soo Hyun Park ◽  
Tae Oh Jeung ◽  
Kee Won Kim ◽  
Jang Hern Lee ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
P38 Mapk ◽  
Adenosine Triphosphate ◽  
Phosphate Uptake ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells

Nephrotoxicity Testing In Vitro: The Current Situation

1997 ◽  
Vol 25 (5) ◽  
pp. 497-503
Author(s):  
Jean-Paul Morin ◽  
Marc E. De Broe ◽  
Walter Pfaller ◽  
Gabriele Schmuck
Keyword(s):  
Proximal Tubule ◽  
Culture Media ◽  
Task Force ◽  
Primary Cultures ◽  
Phenotypic Expression ◽  
Transepithelial Transport ◽  
Specific Cell ◽  
Proximal Tubule Cells ◽  
Tubule Cells

An ECVAM task force on nephrotoxicity has been established to advise, in particular, on the follow-up to recommendations made in the ECVAM workshop report on nephrotoxicity testing in vitro. Since this workshop was held, in 1994, there have been several improvements in the techniques used. For example, the duration of renal slice viability, and the maintenance of functional activities in slices, have been improved by using dynamic incubation systems with higher oxygen tensions and more-appropriate cell culture media. Highly differentiated primary cultures of pig, human and rabbit proximal tubule cells have been established by using specific cell isolation procedures and/or selective culture media. To date, the most comparable phenotypic expression and transepithelial transport capacities to proximal tubules in vivo have been obtained with primary cultures of rabbit proximal tubule cells which are grown on bicompartmental supports; in this system, transepithelial substrate gradients are generated and the transepithelial transport of both organic anions and cations is highly active. This in vitro system has been selected by ECVAM for further evaluation and prevalidation. Industrial needs in the area of nephrotoxicity testing have been identified, and recommendations are made at the end of this report concerning possible future initiatives.

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