Detection of the src-gene product pp60src and its associated protein kinase on the surface of Rous-sarcoma-virus-transformed cells

1980 ◽  
Vol 8 (6) ◽  
pp. 735-736 ◽  
Author(s):  
ANGELIKA BARNEKOW ◽  
BRUCE C. BOSCHEK ◽  
ANDREW ZIEMIECKI ◽  
HEINZ BAUER
Keyword(s):  
Protein Kinase ◽  
Gene Product ◽  
Rous Sarcoma Virus ◽  
Transformed Cells ◽  
Rous Sarcoma ◽  
Src Gene ◽  
Sarcoma Virus

Amino acid alterations within a highly conserved region of the Rous sarcoma virus src gene product pp60src inactivate tyrosine protein kinase activity

1984 ◽  
Vol 4 (5) ◽  
pp. 862-866
Author(s):  
D L Bryant ◽  
J T Parsons
Keyword(s):  
Amino Acid ◽  
Protein Kinase ◽  
Rous Sarcoma Virus ◽  
Kinase Activity ◽  
Mutant Virus ◽  
Protein Kinase Activity ◽  
Rous Sarcoma ◽  
Src Gene ◽  
Tyrosine Protein Kinase ◽  
Sarcoma Virus

Bisulfite mutagenesis techniques have been used to introduce single-point mutations within a region of the Rous sarcoma virus src gene defined by a BglI restriction endonuclease cleavage site. The mutants of Rous sarcoma virus that are produced by these techniques encode src proteins which contain single amino acid changes within a highly conserved amino acid sequence encompassing residues 430 to 433. DNA from the mutants CHpm26 ( Ala430 to Val), CHpm9 ( Pro431 to Ser), CHpm6 ( Glu432 to Lys), and CHpm65 ( Ala433 to Thr) each failed to transform chicken cells upon transfection, whereas DNA from CHpm59 (a third base alteration in the codon for Glu432 ) readily transformed chicken cells. Analysis of immune complexes containing the altered src proteins indicates that these proteins have decreased tyrosine protein kinase activity in vitro. In vivo labeling of cells infected with the mutant virus revealed diminished levels of the tyrosine-phosphorylated 34,000-molecular-weight protein. These data indicate that mutations within the sequence Ala430 - Pro431 - Glu432 - Ala433 lead to alterations in pp60src-specific tyrosine protein kinase activity and a concomitant loss of transforming potential of the mutant virus.

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