Phospholipase C (Bacillus cereus) digestion of phospholipids in myelin isolated from central nervous tissue: Removal of myelin basic protein increases exposure of lipids to the enzyme

1980 ◽  
Vol 8 (5) ◽  
pp. 600-601 ◽  
Author(s):  
KILANI GWARSHA ◽  
MARTIN G. RUMSBY ◽  
CLIVE LITTLE
Keyword(s):  
Bacillus Cereus ◽  
Myelin Basic Protein ◽  
Phospholipase C ◽  
Nervous Tissue ◽  
Basic Protein ◽  
Central Nervous Tissue ◽  
Tissue Removal

Glial Fibrillary Acidic Protein and Myelin Basic Protein as Markers for the Immunochemical Detection of Bovine Central Nervous Tissue in Heat-Treated Meat Products

2005 ◽  
Vol 68 (4) ◽  
pp. 823-827 ◽  
Author(s):  
K. HERDE ◽  
M. BERGMANN ◽  
C. LANG ◽  
R. LEISER ◽  
S. WENISCH
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Myelin Basic Protein ◽  
Nervous Tissue ◽  
Basic Protein ◽  
Meat Products ◽  
The United States ◽  
Acidic Protein ◽  
Spongiform Encephalopathy ◽  
Central Nervous Tissue ◽  
Heat Treated

Because bovine central nervous tissue (CNT) is the main risk material in transmission of the infective agent of bovine spongiform encephalopathy, a suitable test is needed to enforce the ban on CNT in human foodstuffs in the United States and the European Union and to ensure that meat products are free of CNT. To detect bovine CNT in heat-treated meat products, we used immunohistochemistry and Western blots with antibodies against glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP). Both antigens were resistant to processing methods used for meat products. The anti-GFAP antibody showed a high degree of tissue specificity, whereas the anti-MBP antibody had high species specificity, clearly differentiating between porcine and bovine CNT. Therefore, immunochemistry performed with both proteins provides an effective means for detecting bovine CNT in meat products.

scholarly journals Myelin basic protein enhances axonal regeneration from neural progenitor cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhengjian Yan ◽  
Lei Chu ◽  
Xiaojiong Jia ◽  
Lu Lin ◽  
Si Cheng
Keyword(s):  
Myelin Basic Protein ◽  
Neurite Outgrowth ◽  
Progenitor Cells ◽  
Axonal Regeneration ◽  
Neural Progenitor Cells ◽  
Basic Protein ◽  
Neural Progenitor ◽  
Dependent Manner ◽  
Cord Injury

Abstract Introduction Stem cell therapy using neural progenitor cells (NPCs) shows promise in mitigating the debilitating effects of spinal cord injury (SCI). Notably, myelin stimulates axonal regeneration from mammalian NPCs. This led us to hypothesize that myelin-associated proteins may contribute to axonal regeneration from NPCs. Methods We conducted an R-based bioinformatics analysis to identify key gene(s) that may participate in myelin-associated axonal regeneration from murine NPCs, which identified the serine protease myelin basic protein (Mbp). We employed E12 murine NPCs, E14 rat NPCs, and human iPSC-derived Day 1 NPCs (D1 hNPCs) with or without CRISPR/Cas9-mediated Mbp knockout in combination with rescue L1-70 overexpression, constitutively-active VP16-PPARγ2, or the PPARγ agonist ciglitazone. A murine dorsal column crush model of SCI utilizing porous collagen-based scaffolding (PCS)-seeded murine NPCs with or without stable Mbp overexpression was used to assess locomotive recovery and axonal regeneration in vivo. Results Myelin promotes axonal outgrowth from NPCs in an Mbp-dependent manner and that Mbp’s stimulatory effects on NPC neurite outgrowth are mediated by Mbp’s production of L1-70. Furthermore, we determined that Mbp/L1-70’s stimulatory effects on NPC neurite outgrowth are mediated by PPARγ-based repression of neuron differentiation-associated gene expression and PPARγ-based Erk1/2 activation. In vivo, PCS-seeded murine NPCs stably overexpressing Mbp significantly enhanced locomotive recovery and axonal regeneration in post-SCI mice. Conclusions We discovered that Mbp supports axonal regeneration from mammalian NPCs through the novel Mbp/L1cam/Pparγ signaling pathway. This study suggests that bioengineered, NPC-based interventions can promote axonal regeneration and functional recovery post-SCI.

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