The Use of Creatine Kinase Activity as an Index of Skeletal-Muscle Differentiation

1978 ◽  
Vol 6 (3) ◽  
pp. 509-511 ◽  
Author(s):  
GLENN E. MORRIS
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Kinase Activity ◽  
Muscle Differentiation ◽  
Creatine Kinase Activity ◽  
Skeletal Muscle Differentiation

scholarly journals Mitochondrial creatine kinase activity and phosphate shuttling are acutely regulated by exercise in human skeletal muscle

2012 ◽  
Vol 590 (21) ◽  
pp. 5475-5486 ◽  
Author(s):  
Christopher G. R. Perry ◽  
Daniel A. Kane ◽  
Eric A. F. Herbst ◽  
Kazutaka Mukai ◽  
Daniel S. Lark ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Human Skeletal Muscle ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Mitochondrial Creatine Kinase

Creatine kinase:aspartate aminotransferase activity ratio as an indicator of the source of an increased creatine kinase activity.

1988 ◽  
Vol 34 (12) ◽  
pp. 2506-2510 ◽  
Author(s):  
D R Dufour
Keyword(s):  
Myocardial Infarction ◽  
Skeletal Muscle ◽  
Acute Myocardial Infarction ◽  
Creatine Kinase ◽  
Retrospective Study ◽  
Kinase Activity ◽  
Activity Ratio ◽  
Creatine Kinase Activity ◽  
Aminotransferase Activity ◽  
Creatine Kinase Isoenzyme

Abstract Although measurements of creatine kinase isoenzyme 2 (CK-MB) are often used to diagnose acute myocardial infarction, their sensitivity and specificity are less than 100%. Because skeletal muscle contains more CK and less aspartate aminotransferase (AST) than cardiac muscle, the CK/AST ratio might provide a useful adjunct in evaluating the source of a supranormal value for CK. I established the following decision levels in a retrospective study of 342 patients: ratios less than 14 (if total CK was 300-1200 U/L), less than 20 (CK 1201-2000 U/L), or less than 25 (CK greater than 2000 U/L) suggested myocardial infarction, with a sensitivity of 95% and a specificity of 65%. In a validation study with 277 additional patients, liver disease and alcohol abuse caused erroneous results, leading to exclusion of 22% of these patients. In the remaining cases, sensitivity was 94%, specificity 90%. The CK/AST ratios changed little with time, suggesting that a single value would be adequate for evaluating patients with increased CK.

D-2-Hydroxyglutaric acid inhibits creatine kinase activity from cardiac and skeletal muscle of young rats

2003 ◽  
Vol 33 (10) ◽  
pp. 840-847 ◽  
Author(s):  
C. G. Da Silva ◽  
A. R. F. Bueno ◽  
P. F. Schuck ◽  
G. Leipnitz ◽  
C. A. J. Ribeiro ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Young Rats ◽  
Hydroxyglutaric Acid

Gender differences in muscle inflammation after eccentric exercise

2000 ◽  
Vol 89 (6) ◽  
pp. 2325-2332 ◽  
Author(s):  
N. Stupka ◽  
S. Lowther ◽  
K. Chorneyko ◽  
J. M. Bourgeois ◽  
C. Hogben ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Muscle Damage ◽  
Eccentric Exercise ◽  
Kinase Activity ◽  
Morphological Changes ◽  
Vastus Lateralis ◽  
Creatine Kinase Activity ◽  
Blue Staining ◽  
Positive Staining

Unaccustomed exercise is followed by delayed-onset muscle soreness and morphological changes in skeletal muscle. Animal studies have demonstrated that women have an attenuated response to muscle damage. We studied the effect of eccentric exercise in untrained male ( n = 8) and female ( n = 8) subjects using a unilateral exercise design [exercise (Ex) and control (Con) legs]. Plasma granulocyte counts [before (Pre) and 48 h after exercise (+48h)] and creatine kinase activity [Pre, 24 h after exercise (+24h), +48h, and 6 days after exercise (+6d)] were determined before (Pre) and after (+24h, +48h, +6d) exercise, with biopsies taken from the vastus lateralis of each leg at +48h for determination of muscle damage and/or inflammation. Plasma granulocyte counts increased for men and decreased for women at +48h ( P < 0.05), and creatine kinase activity increased for both genders at +48h and +6d ( P < 0.01). There were significantly greater areas of both focal ( P < 0.001) and extensive ( P < 0.01) damage in the Ex vs. Con leg for both genders, which was assessed by using toluidine blue staining. The number of leukocyte common antigen-positive cells/mm2 tissue increased with exercise ( P< 0.05), and men tended to show more in their Ex vs. Con leg compared with women ( P = 0.052). Men had a greater total (Ex and Con legs) number of bcl-2-positive cells/mm2 tissue vs. women ( P < 0.05). Atrophic fibers with homogeneous bcl-2-positive staining were seen only in men ( n = 3). We conclude that muscle damage is similar between genders, yet the inflammatory response is attenuated in women vs. men. Finally, exercise may stimulate the expression of proteins involved in apoptosis in skeletal muscle.

scholarly journals Alterations of skeletal muscle bioenergetics in a mouse with F508del mutation leading to a cystic fibrosis-like condition

2019 ◽  
Vol 317 (2) ◽  
pp. E327-E336
Author(s):  
Nicola Lai ◽  
Chinna Kummitha ◽  
Mitchell Drumm ◽  
Charles Hoppel
Keyword(s):  
Skeletal Muscle ◽  
Cystic Fibrosis ◽  
Creatine Kinase ◽  
Electron Transport ◽  
Oxidative Phosphorylation ◽  
Aerobic Capacity ◽  
Electron Transport Chain ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Transport Chain

High energy expenditure is reported in cystic fibrosis (CF) animal models and patients. Alterations in skeletal muscle oxidative capacity, fuel utilization, and the creatine kinase-phosphocreatine system suggest mitochondrial dysfunction. Studies were performed on congenic C57BL/6J and F508del ( Cftrtm1kth) mice. Indirect calorimetry was used to measure gas exchange to evaluate aerobic capacity during treadmill exercise. The bioenergetic function of skeletal muscle subsarcolemmal (SSM) and interfibrillar mitochondria (IFM) was evaluated using an integrated approach combining measurement of the rate of oxidative phosphorylation by polarography and of electron transport chain activities by spectrophotometry. CF mice have reduced maximal aerobic capacity. In SSM of these mice, oxidative phosphorylation was impaired in the presence of complex I, II, III, and IV substrates except when glutamate was used as substrate. This impairment appeared to be caused by a defect in complex V activity, whereas the oxidative system of the electron transport chain was unchanged. In IFM, oxidative phosphorylation and electron transport chain activities were preserved, whereas complex V activity was reduced, in CF. Furthermore, creatine kinase activity was reduced in both SSM and IFM of CF skeletal muscle. The decreased complex V activity in SSM resulted in reduced oxidative phosphorylation, which could explain the reduced skeletal muscle response to exercise in CF mice. The decrease in mitochondrial creatine kinase activity also contributed to this poor exercise response.

Effect of Hormones on the Development of Creatine Kinase Activity in Rat Skeletal Muscle

Neonatology ◽  
1979 ◽  
Vol 35 (3-4) ◽  
pp. 156-174 ◽  
Author(s):  
H.J. Kloosterboer ◽  
H. van Faassen ◽  
S.A. Stoker-De Vries ◽  
F.A. Hommes
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Rat Skeletal Muscle

TISSUE AND SERUM CREATINE KINASE ACTIVITY IN HYPOTHYROID RATS

1968 ◽  
Vol 42 (4) ◽  
pp. 495-499 ◽  
Author(s):  
F. Q. NUTTALL
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Protein Concentration ◽  
Kinase Activity ◽  
Specific Activity ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Heart Activity ◽  
Total Protein Concentration ◽  
Tissue Extracts

SUMMARY In rats made hypothyroid by thyroidectomy, serum creatine kinase (CK) activity appears to be unchanged or slightly reduced. This is in contrast to the increased values reported in myxoedematous man. Brain CK activity was unchanged and heart activity was only slightly reduced in these animals. Skeletal muscle CK activity, however, was more definitely reduced and the reduction was highly significant. The lower tissue enzyme activity in heart and skeletal muscle could be explained by a reduction in total protein concentration of the tissue extracts from the thyroidectomized animals, since the specific activity of the enzyme from these tissues was essentially unchanged as compared with controls.

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