The Measurement of Ribosomal Ribonucleic Acid Synthesis in Rat Liver and Skeletal Muscle in vivo

1977 ◽  
Vol 5 (4) ◽  
pp. 913-916 ◽  
Author(s):  
GEORGE K. GRIMBLE ◽  
DAVID J. MILLWARD
Keyword(s):  
Skeletal Muscle ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Acid Synthesis ◽  
Ribosomal Ribonucleic Acid

scholarly journals Differences in the patterns of methylation in rat liver ribosomal ribonucleic acid after reaction in vivo with methyl methanesulphonate and NN-dimethylnitrosamine

1972 ◽  
Vol 129 (3) ◽  
pp. 519-528 ◽  
Author(s):  
P. J. O'Connor ◽  
M. J. Capps ◽  
A. W. Craig ◽  
P. D. Lawley ◽  
S. A. Shah
Keyword(s):  
Rat Liver ◽  
Alkaline Hydrolysis ◽  
Ribonucleic Acid ◽  
Significant Feature ◽  
Enzymic Digestion ◽  
Short Intervals ◽  
Ribosomal Ribonucleic Acid ◽  
Methylating Agents ◽  
Hydrolysis Of

1. rRNA was isolated from rat liver at short intervals after the intraperitoneal injection of [14C]methyl methanesulphonate (50mg/kg) or NN-di[14C]methylnitrosamine (2mg/kg). These doses were chosen to minimize the effects of toxicity. 2. The following methods of hydrolysis of [14C]methylated rRNA were employed: enzymic digestion to nucleosides at pH8; alkaline hydrolysis and conversion into nucleosides; acid hydrolysis to bases. 3. The methylation products were analysed by chromatography on columns of Dowex-50 (H+ form) and Dowex-50 (NH4+ form). 4. With both methylating agents the principal product of methylation was 7-methylguanine. Differences were obtained, however, in the molar proportions of the minor bases 3-methylcytosine, 1-methyladenine and 7-methyladenine. Methylation at the O-6 position of guanine was a significant feature of rRNA obtained from the NN-di[14C]methylnitrosamine-treated animals but was not detected in rRNA after treatment with [14C]methyl methanesulphonate.

scholarly journals Ribonucleic acid synthesis in porphyric rat liver induced by 3,5-dicarbethoxy-1,4-dihydrocollidine

1973 ◽  
Vol 136 (1) ◽  
pp. 209-215 ◽  
Author(s):  
Hajime Nawata ◽  
Kenichi Kato
Keyword(s):  
Rat Liver ◽  
Liver Cell ◽  
Ribonucleic Acid ◽  
Rna Synthesis ◽  
Actinomycin D ◽  
Acid Synthesis ◽  
Rapid Decrease ◽  
Aminolaevulinic Acid ◽  
Induction Mechanism

1. Administration of 3,5-dicarbethoxy-1,4-dihydrocollidine to rats caused a marked increase in the activity of δ-aminolaevulinic acid synthetase as well as a slight net increase in RNA in the livers. 2. 3,5-Dicarbethoxy-1,4-dihydrocollidine primarily stimulated the synthesis of RNA in the nucleus of the liver cell. 3. The decrease in RNA synthesis after administration of this drug resulted in a rapid decrease in the activity of this induced enzyme. This was also confirmed by treatment with actinomycin D. 4. From kinetic experiments on synthesis of RNA and δ-aminolaevulinic acid synthetase in vivo the induction mechanism of this enzyme was discussed.

Inhibition by α-amanitin of chromosomal ribonucleic acid synthesis in rat liver

FEBS Letters ◽  
1972 ◽  
Vol 25 (2) ◽  
pp. 305-308 ◽  
Author(s):  
Giulia Montecuccoli ◽  
F. Novello ◽  
F. Stirpe
Keyword(s):  
Rat Liver ◽  
Ribonucleic Acid ◽  
Acid Synthesis
Sign in / Sign up

Export Citation Format

Share Document