Identification of Blood-Sinusoidal Plasma-Membrane Fractions from Rat Liver Homogenates by Radioiodinated-Ligand Binding

1977 ◽  
Vol 5 (1) ◽  
pp. 103-104 ◽  
Author(s):  
FRANK CAREY ◽  
W. HOWARD EVANS
Keyword(s):  
Plasma Membrane ◽  
Ligand Binding ◽  
Rat Liver ◽  
Liver Homogenates

scholarly journals Subcellular fractionation of rat liver homogenates using two-polymer phase systems in a toroidal-coil centrifuge

1984 ◽  
Vol 217 (3) ◽  
pp. 751-759 ◽  
Author(s):  
D Heywood-Waddington ◽  
I A Sutherland ◽  
W B Morris ◽  
T J Peters
Keyword(s):  
Plasma Membrane ◽  
Ethylene Glycol ◽  
Rat Liver ◽  
Flow Rate ◽  
Marker Enzyme ◽  
Poly Ethylene Glycol ◽  
Lower Phase ◽  
Liver Homogenates ◽  
Toroidal Coil ◽  
Poly Ethylene

The principal organelles of rat liver homogenates were fractionated by two-phase partition chromatography using toroidal-coil centrifugation with a mixture of dextran T 500 and poly(ethylene glycol) 6000 in 0.26 M-sucrose containing 10 mM-sodium phosphate/phosphoric acid buffer, pH 7.4. The effects of varying the following parameters on organelle elution profiles, as reflected by their marker-enzyme activities, were studied: centrifuge speed; the composition and relative proportion of dextran-rich and poly(ethylene glycol)-rich phases in the eluent; flow rate; sample volume; homogenate concentration; helix diameter; tubing bore and the number of loops in the coil. Optimal resolution of the organelles was achieved with a toroidal coil of internal diameter 1.07 mm with a 4.55 mm helix diameter on a 0.42 m-diameter rotor running at 1000 rev./min. The eluent was prepared by combining, in a ratio of 93:7 (v/v), the poly(ethylene glycol)-rich upper phase and dextran-rich lower phase obtained from a phase mixture containing 3.3% (w/w) dextran and 5.4% (w/w) poly(ethylene glycol). The flow rate of the eluent was 14ml/h. Optimal conditions for separation of the organelles were evaluated. Resolution of plasma membrane and lysosomes was achieved. Separation of endoplasmic reticulum, which showed marked heterogeneity, from plasma membrane was also demonstrated. DNA and marker enzymes for peroxisomes, mitochondria and cytosol showed distinct elution profiles.

scholarly journals Heterogeneous distribution of enzymes among plasma-membrane fragments sedimenting with the microsomal fraction of rat liver

1974 ◽  
Vol 142 (3) ◽  
pp. 667-671 ◽  
Author(s):  
Kenneth A. Norris ◽  
Miloslav Dobrota ◽  
Faiz S. Issa ◽  
Richard H. Hinton ◽  
Eric Reid
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Microsomal Fraction ◽  
Heterogeneous Distribution ◽  
Similar Product ◽  
Liver Homogenates

Plasma-membrane fragments recovered in the microsomal fraction of rat liver homogenates were shown to be heterogeneous in density. It was demonstrated that 5′-nucleotidase, the most commonly used plasma-membrane marker, is concentrated in the lightest subfraction. Two of the published procedures for the isolation of plasma-membrane fragments from the microsomal fraction (Touster et al., 1970; Hinton et al., 1971) are shown to give products which are not representative of all the plasma-membrane fragments of microsomal size, and it is argued that a third procedure (House & Weidemann, 1970) is likely to give a similar product.

scholarly journals THE INCORPORATION OF THE CARBOXYL CARBON FROM ACETATE INTO CHOLESTEROL BY RAT LIVER HOMOGENATES

1954 ◽  
Vol 206 (1) ◽  
pp. 471-481 ◽  
Author(s):  
Ivan D. Frantz ◽  
Nancy L.R. Bucher ◽  
Henny S. Schneider ◽  
Naomi H. McGovern ◽  
Ruth Kingston
Keyword(s):  
Rat Liver ◽  
Liver Homogenates ◽  
Carboxyl Carbon

scholarly journals Glucagon-(19-29), a Ca2+ pump inhibitory peptide, is processed from glucagon in the rat liver plasma membrane by a thiol endopeptidase.

1990 ◽  
Vol 265 (35) ◽  
pp. 21514-21519 ◽  
Author(s):  
P Blache ◽  
A Kervran ◽  
M Dufour ◽  
J Martinez ◽  
D Le-Nguyen ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Inhibitory Peptide ◽  
Liver Plasma Membrane

scholarly journals STIMULATION BY DINITROPHENOL OF FORMATION OF MELANIN-LIKE SUBSTANCE FROM TYROSINE BY RAT LIVER HOMOGENATES

1957 ◽  
Vol 225 (2) ◽  
pp. 735-744
Author(s):  
Henry Kamin ◽  
Mildred A. Koon ◽  
Philip Handler
Keyword(s):  
Rat Liver ◽  
Liver Homogenates
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