An Improved Working Rat Heart Preparation and a New Apparatus for Pyruvate Dehydrogenase Determinations

1976 ◽  
Vol 4 (2) ◽  
pp. 277-279 ◽  
Author(s):  
JOHN A. ILLINGWORTH ◽  
ROSANNE MULLINGS
Keyword(s):  
Pyruvate Dehydrogenase ◽  
Rat Heart ◽  
New Apparatus ◽  
Heart Preparation ◽  
Working Rat Heart

Isolated biventricular working rat heart preparation

1992 ◽  
Vol 54 (5) ◽  
pp. 915-920 ◽  
Author(s):  
Todd L. Demmy ◽  
George J. Magovern ◽  
Race L. Kao
Keyword(s):  
Rat Heart ◽  
Heart Preparation ◽  
Working Rat Heart

In VitroAssessment of Cardiac Performance after Irradiation Using an Isolated Working Rat Heart Preparation

1991 ◽  
Vol 59 (4) ◽  
pp. 1053-1068 ◽  
Author(s):  
J. Wondergem ◽  
A. Van Der Laarse ◽  
F.J.M. Van Ravels ◽  
A.-M. Van Wermeskerken ◽  
H.R. Verhoeve ◽  
...  
Keyword(s):  
Rat Heart ◽  
Cardiac Performance ◽  
Heart Preparation ◽  
Working Rat Heart

Effect of thyroxine treatment on exogenous myocardial lactate oxidation

1982 ◽  
Vol 243 (5) ◽  
pp. H722-H728
Author(s):  
M. Fintel ◽  
A. H. Burns
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Dehydrogenase Activity ◽  
Pyruvate Dehydrogenase ◽  
Acid Oxidation ◽  
Lactate Oxidation ◽  
Thyroxine Treatment ◽  
Heart Preparation ◽  
Mitochondrial Oxidation ◽  
Working Rat Heart

The effect of thyroxine treatment on myocardial lactate oxidation was examined by use of an isolated, working rat heart preparation. Thyroxine treatment, both acute and chronic, was associated with a decrease in lactate oxidation, when the heart was perfused with a physiological blend of substrates (free fatty acids, lactate, and glucose). This decrease in lactate oxidation was not caused by a generalized impairment in mitochondrial oxidation of acetyl coenzyme A (CoA), as oxygen consumption was normal and fatty acid oxidation was elevated in the treated animals. The block in lactate oxidation was localized to the conversion of pyruvate to acetyl CoA, as indicated by the depressed oxidation of pyruvate and lactate. Thyroxine treatment was associated with a decrease in pyruvate dehydrogenase activity. The decrease in pyruvate dehydrogenase activity was reversible and was attributed to the enhanced myocardial oxidation of free fatty acids.

Pharmacological evidence for catecholamine-independent contractile effects of X-537A on the isolated working rat heart preparation

1979 ◽  
Vol 57 (4) ◽  
pp. 428-431 ◽  
Author(s):  
Robert L. Rodgers ◽  
Joanne I. Moore ◽  
K. Roger Hornbrook
Keyword(s):  
Heart Rate ◽  
Rat Heart ◽  
Independent Component ◽  
The Other ◽  
Inotropic Effect ◽  
Inotropic Response ◽  
Other Hand ◽  
Heart Preparation ◽  
Working Rat Heart

The ionophore X-537A increased heart rate and contractility of the isolated, working rat heart preparation. The increased heart rate appeared to be caused solely by release of catecholamines as the response was completely eliminated by reserpine pretreatment or addition of propranolol to the perfusate. The inotropic response, however, had an apparent catecholamine-independent component as neither propranolol, nor propranolol in combination with phentolamine, completely eliminated the inotropic response to X-537A. On the other hand, reserpine pretreatment did abolish the inotropic effect of the ionophore but this action appeared to be a nonspecific one as the responses to norepinephrine and to CaCl2 were substantially diminished.

Effects of aging on the activities of pyruvate dehydrogenase complex and its kinase in rat heart

Life Sciences ◽  
1997 ◽  
Vol 60 (25) ◽  
pp. 2309-2314 ◽  
Author(s):  
Naoya Nakai ◽  
Yuzo Sato ◽  
Yoshiharu Oshida ◽  
Atsushi Yoshimura ◽  
Noriaki Fujitsuka ◽  
...  
Keyword(s):  
Pyruvate Dehydrogenase ◽  
Rat Heart ◽  
Pyruvate Dehydrogenase Complex ◽  
Effects Of Aging ◽  
Dehydrogenase Complex
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