A Saturation Assay Method for Adenosine 3′:5′-Cyclic Monophosphate in Plasma and its Use in Studies of the Action of Bovine Parathyroid Hormone

1974 ◽  
Vol 2 (3) ◽  
pp. 453-455 ◽  
Author(s):  
PETER M. BARLING ◽  
JANET D. M. ALBANO ◽  
STEPHEN TOMLINSON ◽  
BARRY L. BROWN ◽  
J. L. H. O'RIORDAN
Keyword(s):  
Parathyroid Hormone ◽  
Assay Method ◽  
Cyclic Monophosphate ◽  
Bovine Parathyroid Hormone

Plasma adenosine 3′, 5′-cyclic monophosphate: Dose-response to injected bovine parathyroid hormone in man

1976 ◽  
Vol 22 (S1) ◽  
pp. 332-335 ◽  
Author(s):  
I. G. Lewin ◽  
G. N. Hendy ◽  
S. E. Papapoulos ◽  
S. Tomlinson ◽  
J. L. H. O'Riordan
Keyword(s):  
Parathyroid Hormone ◽  
Dose Response ◽  
Cyclic Monophosphate ◽  
Plasma Adenosine ◽  
Bovine Parathyroid Hormone

The Effects of Exogenous Parathyroid Hormone on Plasma and Urinary Adenosine 3′,5′-Cyclic Monophosphate in Man

1974 ◽  
Vol 47 (5) ◽  
pp. 481-492 ◽  
Author(s):  
S. Tomlinson ◽  
P. M. Barling ◽  
J. D. M. Albano ◽  
B. L. Brown ◽  
J. L. H. O'Riordan
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Bolus Injection ◽  
Normal Subjects ◽  
Peripheral Vein ◽  
Terminal Part ◽  
Cyclic Monophosphate ◽  
Peripheral Plasma ◽  
Bovine Parathyroid Hormone ◽  
Subsequent Disappearance

1. Administration of highly purified bovine parathyroid hormone (BPTH) (200 MRC units) increased the concentration of adenosine 3′,5′-cyclic monophosphate (cyclic AMP) in the peripheral plasma of normal subjects within minutes, whether the hormone was given as a bolus injection or an infusion. 2. The subsequent disappearance of cyclic AMP from the circulation was also rapid (t1/2 = 14 min) and was associated with a prompt decline (t1/2 = 4.6 min) in the concentration of the ammo-terminal part of BPTH, as measured in a region-specific immunoradiometric assay. 3. The concentration of cyclic AMP in plasma from a renal vein was found to increase more rapidly and to reach a greater peak than plasma from a peripheral vein. 4. The administration of BPTH to anephric subjects caused no increase in cyclic AMP. 5. These investigations indicate that parathyroid hormone can act extremely rapidly, with a short half-life, and that the kidney makes a major contribution to the changes in plasma cyclic AMP induced by the hormone. In addition, they form the basis for the development of a simplified Ellsworth—Howard test, using changes in circulating cyclic AMP after BPTH administration as an index of responsiveness to the hormone.

scholarly journals The Effect of Bovine Parathyroid Hormone Withdrawal on MC3T3-E1 Cell Proliferation and Phosphorus Metabolism

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0120402 ◽  
Author(s):  
Shuangxin Liu ◽  
Weiping Zhu ◽  
Sijia Li ◽  
Tongxia Cui ◽  
Zhonghe Li ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Parathyroid Hormone ◽  
Phosphorus Metabolism ◽  
Bovine Parathyroid Hormone

Peripheral metabolism of bovine parathyroid hormone in the dog

Metabolism ◽  
1975 ◽  
Vol 24 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Frederick R. Singer ◽  
Gino V. Segre ◽  
Joel F. Habener ◽  
John T. Potts
Keyword(s):  
Parathyroid Hormone ◽  
Peripheral Metabolism ◽  
Bovine Parathyroid Hormone

IMMUNORADIOMETRIC ASSAY OF PARATHYROID HORMONE

1971 ◽  
Vol 49 (3) ◽  
pp. 521-530 ◽  
Author(s):  
G. M. ADDISON ◽  
C. N. HALES ◽  
J. S. WOODHEAD ◽  
J. L. H. O'RIORDAN
Keyword(s):  
Parathyroid Hormone ◽  
Serum Concentration ◽  
Immunoradiometric Assay ◽  
Human Parathyroid Hormone ◽  
Calibration Curves ◽  
High Concentrations ◽  
Bovine Parathyroid Hormone

SUMMARY An immunoradiometric assay for parathyroid hormone has been developed. Antisera to bovine parathyroid hormone (BPTH) were screened for their ability to bind BPTH and human parathyroid hormone (HPTH). A BPTH-immunoadsorbent was used to extract antibodies from an antiserum which did not discriminate between BPTH and HPTH in a standard radioimmunoassay. These antibodies were labelled with 125I for use in the immunoradiometric assay. With this system as little as 5 pg BPTH and 8 pg HPTH could be detected. The serum concentration of BPTH was shown to rise in a cow rendered hypocalcaemic by an infusion of EDTA. Sera from patients with hyperparathyroidism contained high concentrations of hormone. These sera were found to dilute-out parallel to calibration curves obtained using HPTH extracted from parathyroid adenomata. The advantages of this method over the standard radioimmunoassay are discussed.

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