Stimulation of Phospholipase A2 of Synaptosomes of Guinea-Pig Brain by Noradrenaline and other Amines

1973 ◽  
Vol 1 (4) ◽  
pp. 849-849 ◽  
Author(s):  
R. J. GULLIS ◽  
C. E. ROWE
Keyword(s):  
Guinea Pig ◽  
Phospholipase A2 ◽  
Pig Brain ◽  
Guinea Pig Brain ◽  
Stimulation Of

The effect of diazepam on adenosine uptake and adenosine-stimuiated adenylate cyclase in guinea-pig brain

1982 ◽  
Vol 60 (3) ◽  
pp. 302-307 ◽  
Author(s):  
M. J. York ◽  
L. P. Davies
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Adenosine Deaminase ◽  
Small Component ◽  
Extracellular Adenosine ◽  
Pig Brain ◽  
Adenosine Uptake ◽  
Adenosine Antagonist ◽  
Guinea Pig Brain ◽  
Stimulation Of

We have used the adenosine-stimulated adenylate cyclase of guinea-pig brain to examine the potency of diazepam as an adenosine uptake inhibitor. Diazepam at concentrations in the range 10–500 μM stimulates the production of cAMP in incubated slices of guinea-pig cerebral cortex, with maximal fivefold stimulations over basal levels by 200 μM diazepam. The increases can be largely (but not completely) blocked by the adenosine antagonist theophylline or by addition of excess adenosine deaminase to the system. It appears that the stimulation of cAMP production is due to a blockade of adenosine uptake which results in an increase in extracellular adenosine and concomitant activation of the adenosine receptor coupled to adenylate cyclase. Since the cAMP response to standard adenosine uptake blockers (dipyridamole, dilazep) can be completely blocked by theophylline or adenosine deaminase, a small component of the diazepam response cannot be explained by an adenosine effect. The concentration of diazepam at which the first significant cAMP increase occurs is 10 μM or slightly lower. This is significantly higher than the concentration of diazepam needed to saturate the pharmacologically characterized central nervous system receptors for benzodiazepines.

scholarly journals Stimulation of the production of unesterified fatty acids in nerve endings of guinea-pig brain in vitro by noradrenaline and 5-hydroxytryptamine

1972 ◽  
Vol 126 (3) ◽  
pp. 575-585 ◽  
Author(s):  
C. J. Price ◽  
C. E. Rowe
Keyword(s):  
Fatty Acids ◽  
Cerebral Cortex ◽  
Guinea Pig ◽  
Synaptic Vesicles ◽  
Nerve Endings ◽  
Synaptic Membranes ◽  
Pig Brain ◽  
Guinea Pig Brain ◽  
Stimulation Of

1. Noradrenaline (1mm) and 5-hydroxytryptamine (1mm) stimulated the production of unesterified palmitate, oleate, stearate and arachidonate in nerve endings (synaptosomes) isolated from combined guinea-pig cerebral cortex and cerebellum. 2. Iproniazid phosphate (0.36mm) increased the concentrations of the same acids in osmotically ruptured synaptosomes. Further addition of 1mm-noradrenaline or 1mm-5-hydroxytryptamine reversed this increase. 3. Noradrenaline (0.01mm) stimulated the production of unesterified fatty acids in isolated synaptic membranes. 5-Hydroxytryptamine (0.01mm) stimulated the production of unesterified fatty acids in synaptic membranes and synaptic vesicles.

scholarly journals NMDA RECEPTOR DOWN-REGULATION BY PHOSPHOLIPASE A2 IN THE FETAL GUINEA PIG BRAIN DURING HYPOXIA. • 2248

1996 ◽  
Vol 39 ◽  
pp. 377-377
Author(s):  
Om P Mishra ◽  
David Moon ◽  
Anli Zhu ◽  
Jane E McGowan ◽  
Maria Delivoria-Papadopoulos
Keyword(s):  
Nmda Receptor ◽  
Guinea Pig ◽  
Phospholipase A2 ◽  
Down Regulation ◽  
Pig Brain ◽  
Guinea Pig Brain

Stimulation of guinea-pig brain adenylate cyclase by adenosine analogues with potent pharmacological activity

Life Sciences ◽  
1980 ◽  
Vol 26 (13) ◽  
pp. 1079-1088 ◽  
Author(s):  
Les P. Davies ◽  
Kenneth M. Taylor ◽  
Richard P. Gregson ◽  
Ronald J. Quinn
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Pharmacological Activity ◽  
Adenosine Analogues ◽  
Pig Brain ◽  
Guinea Pig Brain ◽  
Stimulation Of

scholarly journals The stimulation by transmitter substances and putative transmitter substances of the net activity of phospholipase A2 of synaptic membranes of cortex of guinea-pig brain

1975 ◽  
Vol 148 (2) ◽  
pp. 197-208 ◽  
Author(s):  
R J Gullis ◽  
C E Rowe
Keyword(s):  
Guinea Pig ◽  
Phospholipase A2 ◽  
Synaptic Vesicles ◽  
Optimum Concentration ◽  
Synaptic Membranes ◽  
Phospholipase A1 ◽  
Response Curves ◽  
Pig Brain ◽  
Hydrolysis Of ◽  
Guinea Pig Brain

1. The distribution of the hydrolyses of phosphatidylcholine by phospholipase A2 and phospholipase A1, and the hydrolysis of lysophosphatidylcholine by lysophospholipase, in subcellular and subsynaptosomal fractions of cerebral cortices of guinea-pig brain, was determined. 2. Noradrenaline stimulated hydrolysis by phospholipase A2 in whole synaptosomes, synaptic membranes and fractions containing synaptic vesicles. 3. Stimulation of hydrolysis by phospholipase A2 in synaptic membranes by noradrenaline was enhanced by CaCl2, and by a mixture of ATP and MgCl2. The optimum concentration of CaCl2, in the presence of ATP and MgCl2, for stimulation by 10 muM-noradrenaline was in the range 1-10muM. The optimum concentration for ATP-2MgCl2 in the presence of 1 muM-CaCl2 was in the range 0.1-1mM. 4. Hydrolysis by phospholipase A2 of synaptic membranes was also stimulated by acetylcholine, carbamoylcholine, 5-hydroxytryptamine, dopamine (3,4-dihydroxyphenethylamine), histamine, psi-aminobutyric acid, glutamic acid and aspartic acid. With appropriate concentrations of cofactors, sigmoidal dose-response curves were obtained, half-maximum stimulations being obtained with concentrations of stimulant in the range 0.1-1muM. 5. Taurine also stimulated hydrolysis of phosphatidylcholine by phospholipase A2. There were only slight stimulations with methylamine, ethylenediamine or spermidine. No stimulation was obtained with glucagon.

scholarly journals Communication calls produced by electrical stimulation of four structures in the guinea pig brain

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0194091 ◽  
Author(s):  
David B. Green ◽  
Trevor M. Shackleton ◽  
Jasmine M. S. Grimsley ◽  
Oliver Zobay ◽  
Alan R. Palmer ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Guinea Pig ◽  
Pig Brain ◽  
Communication Calls ◽  
Guinea Pig Brain ◽  
Stimulation Of

scholarly journals Distribution of the Olfactory Fiber Input Into the Olfactory Tubercle of the In Vitro Isolated Guinea Pig Brain

2009 ◽  
Vol 101 (3) ◽  
pp. 1613-1619 ◽  
Author(s):  
Giovanni Carriero ◽  
Laura Uva ◽  
Vadym Gnatkovsky ◽  
Marco de Curtis
Keyword(s):  
Guinea Pig ◽  
Piriform Cortex ◽  
Field Potential ◽  
Lateral Part ◽  
Olfactory Tubercle ◽  
Olfactory Tract ◽  
Pig Brain ◽  
Guinea Pig Brain ◽  
Stimulation Of

The olfactory tubercle (OT) is a cortical component of the olfactory system involved in reward mechanisms of drug abuse. This region covers an extensive part of the rostral ventral cerebrum and is relatively poorly studied. The intrinsic network interactions evoked by olfactory input are analyzed in the OT of the in vitro isolated guinea pig brain by means of field potential analysis and optical imaging of voltage-sensitive signals. Stimulation of the lateral olfactory tract induces a monosynaptic response that progressively decreases in amplitude from lateral to medial. The monosynaptic input induces a disynaptic response that is proportionally larger in the medial portion of the OT. Direct stimulation of the piriform cortex and subsequent lesion of this pathway showed the existence of an associative disynaptic projection from the anterior part of the piriform cortex to the lateral part of the OT that integrates with the component mediated by the local intra-OT collaterals. Optical and electrophysiological recordings of the signals evoked by stimulation of the olfactory tract during arterial perfusion with the voltage-sensitive dye di-2-ANEPEQ confirmed the pattern of distribution of the mono and disynaptic responses in the OT. Finally, current source density analysis of laminar profiles recorded with 16-channel silicon probes confirmed that the monosynaptic and disynaptic potentials localize in the most superficial and the deep portions of the plexiform layer I, as suggested by previous reports. This study sets the standard for further analysis of the modulation of network properties in this largely unexplored brain region.

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