scholarly journals Extracellular regulated kinase 5 mediates osteoporosis through modulating viability and apoptosis of osteoblasts in ovariectomized rats

2019 ◽  
Vol 39 (9) ◽  
Author(s):  
Tuan-Mao Guo ◽  
Yan-Li Xing ◽  
Hai-Yun Zhu ◽  
Lan Yang ◽  
Guo-Xiong Liu ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Postmenopausal Osteoporosis ◽  
Biomechanical Properties ◽  
Ovariectomized Rats ◽  
Sprague Dawley ◽  
Mineral Density ◽  
Alp Activity ◽  
Extracellular Signal ◽  
Osteoblast Apoptosis ◽  
Turnover Markers

Abstract Postmenopausal osteoporosis is a common condition characterized by the increase and activation of osteoclasts. The present study aimed to investigate the effects of extracellular signal-regulated kinase (ERK) 5 (ERK-5) on postmenopausal osteoporosis by regulating the biological behaviors of osteoblasts. Sprague–Dawley (SD) rats were ovariectomized to develop an osteoporosis model. A lentivirus packaging system was employed to generate lentiviruses capable of up- or down-regulating the expression of ERK-5 in ovariectomized rats. The femoral biomechanical properties, bone mineral density (BMD), contents of calcium (Ca), phosphorus (P) and alkaline phosphatase (ALP) and bone turnover markers in rats, as well as viability, cycle and apoptosis of osteoblasts and ALP activity in osteoblasts were measured in the ovariectomized rats so as to explore the functional significance of ERK-5 in postmenopausal osteoporosis. The femoral mechanical strength of ovariectomized rats was enhanced by overexpression of ERK-5. Meanwhile femoral BMD, and bone metabolism were increased, and bone turnover normalized in the ovariectomized rats when ERK-5 was overexpressed. Lentivirus-mediated ERK-5 overexpression in osteoblasts was observed to inhibit osteoblast apoptosis, and promote viability, accompanied with increased ALP activity. Taken together, ERK-5 could decelerate osteoblast apoptosis and improve postmenopausal osteoporosis by increasing osteoblast viability. Thus, our study provides further understanding on a promising therapeutic target for postmenopausal osteoporosis.

scholarly journals Serumβ-Catenin Levels Associated with the Ratio of RANKL/OPG in Patients with Postmenopausal Osteoporosis

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Xiao-Juan Xu ◽  
Lin Shen ◽  
Yan-Ping Yang ◽  
Rui Zhu ◽  
Bo Shuai ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Postmenopausal Women ◽  
Postmenopausal Osteoporosis ◽  
Bone Turnover Markers ◽  
Enzyme Linked Immunosorbent Assay ◽  
Functional Communication ◽  
Mineral Density ◽  
Wnt Pathways ◽  
Turnover Markers

Objective. To demonstrate the role of Wnt/β-catenin canonical pathway in postmenopausal osteoporosis by evaluating serumβ-catenin levels in patients with postmenopausal osteoporosis and analyzing their possible relationship with serum OPG, RANKL, the ratio of RANKL/OPG, sclerostin, and bone turnover markers.Methods. 480 patients with postmenopausal osteoporosis and 170 healthy postmenopausal women were enrolled in the study. Serumβ-catenin, OPG, RANKL, and sclerostin levels were measured by enzyme-linked immunosorbent assay. Bone status was assessed by measuring bone mineral density and bone turnover markers. Estradiol levels were also detected.Results. Serumβ-catenin levels were lower in postmenopausal osteoporotic women compared to nonosteoporotic postmenopausal women (26.26±14.81versus39.33±5.47 pg/mL,P<0.001). Serumβ-catenin was positively correlated with osteoprotegerin (r=0.232,P<0.001) and negatively correlated with the ratio of RANKL/OPG, body mass index, and sclerostin (r=-0.128,P=0.005;r=-0.117,P=0.010;r=-0.400,P<0.001, resp.) in patients with postmenopausal osteoporosis.Conclusion. The results indicate that lower serumβ-catenin and concomitantly higher ratio of RANKL/OPG may be involved in the pathogenesis of postmenopausal osteoporosis. Functional communication between RANKL/RANK/OPG system and Wnt pathways plays an important role in postmenopausal osteoporosis.

scholarly journals Association between Postmenopausal Osteoporosis and Experimental Periodontitis

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Kai Luo ◽  
Souzhi Ma ◽  
Jianbin Guo ◽  
Yongling Huang ◽  
Fuhua Yan ◽  
...  
Keyword(s):  
Postmenopausal Osteoporosis ◽  
Alveolar Bone ◽  
Ovariectomized Rats ◽  
Whole Body ◽  
Inflammatory Factors ◽  
Mineral Density ◽  
Metabolic Markers ◽  
Periodontal Tissues ◽  
Experimental Periodontitis ◽  
Turnover Markers

To investigate the correlation between postmenopausal osteoporosis (PMO) and the pathogenesis of periodontitis, ovariectomized rats were generated and the experimental periodontitis was induced using a silk ligature. The inflammatory factors and bone metabolic markers were measured in the serum and periodontal tissues of ovariectomized rats using an automatic chemistry analyzer, enzyme-linked immunosorbent assays, and immunohistochemistry. The bone mineral density of whole body, pelvis, and spine was analyzed using dual-energy X-ray absorptiometry and image analysis. All data were analyzed using SPSS 13.0 statistical software. It was found that ovariectomy could upregulate the expression of interleukin- (IL-)6, the receptor activator of nuclear factor-κB ligand (RANKL), and osteoprotegerin (OPG) and downregulate IL-10 expression in periodontal tissues, which resulted in progressive alveolar bone loss in experimental periodontitis. This study indicates that changes of cytokines and bone turnover markers in the periodontal tissues of ovariectomized rats contribute to the damage of periodontal tissues.

The three-year effect of a single zoledronate infusion on bone mineral density and bone turnover markers following denosumab discontinuation in women with postmenopausal osteoporosis

Bone ◽  
2020 ◽  
Vol 138 ◽  
pp. 115478 ◽  
Author(s):  
Polyzois Makras ◽  
Socrates E. Papapoulos ◽  
Stergios A. Polyzos ◽  
Natasha M. Appelman-Dijkstra ◽  
Athanasios D. Anastasilakis
Keyword(s):  
Bone Mineral Density ◽  
Bone Turnover ◽  
Postmenopausal Osteoporosis ◽  
Bone Mineral ◽  
Bone Turnover Markers ◽  
Mineral Density ◽  
Year Effect ◽  
Turnover Markers

THE EFFECTS OF SQUARE DANCING ON BONE MINERAL DENSITY AND BONE TURNOVER MARKERS IN PATIENTS WITH POSTMENOPAUSAL OSTEOPOROSIS

2018 ◽  
Vol 18 (08) ◽  
pp. 1840027 ◽  
Author(s):  
JINZE QIN ◽  
XIAOXU RONG ◽  
GUOXING ZHU ◽  
YU JIANG
Keyword(s):  
Bone Turnover ◽  
Postmenopausal Osteoporosis ◽  
Research Group ◽  
Bone Turnover Markers ◽  
Bone Pain ◽  
Serum Levels ◽  
Control Group ◽  
Mineral Density ◽  
Before And After ◽  
Turnover Markers

Objective: To study the effects of square dance on bone mineral density (BMD) and bone turnover markers in patients with postmenopausal osteoporosis. Methods: Fifty postmenopausal women (aging 45–60) with osteoporosis were randomly assigned to a research group and a control group. The patients in the research group received 600[Formula: see text]mg of calcium combined with square dance (5 times a week, 30 to 60 minutes each time) for 6 months while those in the control group only received 600[Formula: see text]mg of calcium. BMD, changes in bone turnover markers and bone pain were assessed before and after the treatment. Results: (1) After the 6-month treatment, BMD of L[Formula: see text] and the femoral neck significantly increased in the research group ([Formula: see text]). No significant change was observed in BMD of the Ward’s region. BMD in the control group did not change significantly ([Formula: see text]). (2) No significant difference was found in serum levels of calcium, phosphorus and alkaline phosphatase between the two groups both before and after the treatment ([Formula: see text]). Serum levels of P1NP had a significant increase, while [Formula: see text]-CTX’s level did not change significantly. (3) The bone pain in both groups has been effectively relieved. The pain in the research group was relieved significantly compared to the control group. Conclusions: By improving the BMD and relieving the pain, square dancing can exert positive effects on women with postmenopausal osteoporosis. Square dancing may become a feasible strategy for osteoporosis prevention and treatment.

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