scholarly journals A promising growth promoting Meyerozyma caribbica from Solanum xanthocarpum alleviated stress in maize plants

2019 ◽  
Vol 39 (10) ◽  
Author(s):  
Farzana Gul Jan ◽  
Muhammad Hamayun ◽  
Anwar Hussain ◽  
Amjad Iqbal ◽  
Gul Jan ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salinity Stress ◽  
Fungal Endophytes ◽  
Relative Water ◽  
Plant Growth Promoting ◽  
Dpph Scavenging Activity ◽  
Maize Plants ◽  
Growth Promoting ◽  
Dpph Scavenging ◽  
Solanum Xanthocarpum

Abstract Fungal endophytes are known to secrete a large array of secondary metabolites (phenols, flavonoids, indole acetic acid (IAA) etc.) that facilitate crops under stress conditions. Considering this, a potent plant growth promoting endophyte (SXSp1) from the spines and leaves of Solanum xanthocarpum L. has been isolated. The isolated strain ably secreted high quantities of indole-3-acetic acid, phenols and flavonoids. Also, it exhibited phosphate solubilization, siderophore and had 2,2 diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. The SXSp1 also resisted the salinity stress up to 150 mM. LC/MS analysis of SXSp1 culture filtrate (CF) revealed the presence of p-hydroxyl benzoic acid, diadzein, genistien, myricetin and caffeoyl-d-glucose. Moreover, the inoculation of maize plants with SXSp1 significantly (P=0.05) promoted the chlorophyll and carotenoid contents, root and shoot lengths, plant fresh and dry weights, catalase and peroxidase activities, proline, phenolics, flavonoids and relative water contents (RWCs) under salinity. More interestingly, SXSp1-associated plants showed lower endogenous abscisic acid (ABA) and higher endogenous IAA contents that helped the plants to resist salinity stress up to 100 mM. After sequencing, internal transcribed spacer (ITS) regions (ITS1 and ITS4) and phylogenetic analysis, the SXSp1 was identified as Meyerozyma caribbica.

scholarly journals Silicon and Plant Growth-Promoting Rhizobacteria Pseudomonas psychrotolerans CS51 Mitigates Salt Stress in Zea mays L.

Agriculture ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 272
Author(s):  
Happy Anita Appiah Kubi ◽  
Muhammad Aaqil Khan ◽  
Arjun Adhikari ◽  
Muhammad Imran ◽  
Sang-Mo Kang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Salinity Stress ◽  
Nacl Stress ◽  
Luria Bertani ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Maize Plants ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Salinity is a significant abiotic stress for crop plants and a threat to global food security. Optimizing yield without adversely affecting the ecosystem is necessary for a sustainable agriculture. Silicon and plant growth-promoting bacteria were reported for mitigating several abiotic and biotic stress in plants. In our study, we identified the salt-tolerant rhizobacterium Pseudomonas psychrotolerans CS51. This species produces several plant-growth-promoting biochemicals like indole-3-acetic acid (33 ± 1.8 ng/mL) and gibberellic acid (GA3; 38 ± 1.3 and GA4; 23 ± 1.2 ng/mL) in Luria-Bertani(LB) media, and LB media spiked with 200 mM NaCl (indole-3-acetic acid(IAA); 17.6 ± 0.4 ng/mL, GA3; 21 ± 0.9 and GA4; 19 ± 1.0 ng/mL). In the current study, we aimed to investigate the effect of isolate CS51 and exogenous silicon (3 mM) on maize under salinity stress (200 mM). Our results showed that the sole application of isolate CS51, Si, and combined CS51 + Si significantly enhanced maize biomass and chlorophyll content under normal and salinity stress. Phytohormonal results showed that salinity stress increased abscisic acid (ABA; three folds) and jasmonic acid (JA; 49.20%). However, the sole and combined isolate CS51 + Si application markedly reduced ABA (1.5 folds) and JA content (14.89%). Besides, the sole and isolate CS51 + Si co-application strengthened the antioxidant system, such as flavonoid (97%) and polyphenol (19.64%), and lowered the proline content (57.69%) under NaCl stress. Similarly, the CS51 and Si inoculation (solely or combined) significantly enhanced the Si uptake (4 folds) and reduced the Na+ uptake (42.30%) in maize plants under NaCl stress. In conclusion, the current finding suggests that combining CS51 with Si can be used against salinity stress in maize plants and may be commercialized as a biofertilizer.

scholarly journals Isolation and Characterization of Fungal Endophytes Isolated from Medicinal Plant Ephedra pachyclada as Plant Growth-Promoting

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 140 ◽  
Author(s):  
Ahmed Mohamed Aly Khalil ◽  
Saad El-Din Hassan ◽  
Sultan M. Alsharif ◽  
Ahmed M. Eid ◽  
Emad El-Din Ewais ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Plant Growth ◽  
Aspergillus Flavus ◽  
Endophytic Fungi ◽  
Fungal Endophytes ◽  
Penicillium Crustosum ◽  
Plant Growth Promoting ◽  
Penicillium Commune ◽  
Maize Plants ◽  
Growth Promoting

Endophytic fungi are widely present in internal plant tissues and provide different benefits to their host. Medicinal plants have unexplored diversity of functional fungal association; therefore, this study aimed to isolate endophytic fungi associated with leaves of medicinal plants Ephedra pachyclada and evaluate their plant growth-promoting properties. Fifteen isolated fungal endophytes belonging to Ascomycota, with three different genera, Penicillium, Alternaria, and Aspergillus, were obtained from healthy leaves of E. pachyclada. These fungal endophytes have varied antimicrobial activity against human pathogenic microbes and produce ammonia and indole acetic acid (IAA), in addition to their enzymatic activity. The results showed that Penicillium commune EP-5 had a maximum IAA productivity of 192.1 ± 4.04 µg mL−1 in the presence of 5 µg mL−1 tryptophan. The fungal isolates of Penicillium crustosum EP-2, Penicillium chrysogenum EP-3, and Aspergillus flavus EP-14 exhibited variable efficiency for solubilizing phosphate salts. Five representative fungal endophytes of Penicillium crustosum EP-2, Penicillium commune EP-5, Penicillium caseifulvum EP-11, Alternaria tenuissima EP-13, and Aspergillus flavus EP-14 and their consortium were selected and applied as bioinoculant to maize plants. The results showed that Penicillium commune EP-5 increased root lengths from 15.8 ± 0.8 to 22.1 ± 0.6. Moreover, the vegetative growth features of inoculated maize plants improved more than the uninoculated ones.

scholarly journals Acetobacter sacchari sp. nov., for a plant growth-promoting acetic acid bacterium isolated in Vietnam

2019 ◽  
Vol 69 (11) ◽  
pp. 1155-1163 ◽  
Author(s):  
Huong Thi Lan Vu ◽  
Pattaraporn Yukphan ◽  
Van Thi Thu Bui ◽  
Piyanat Charoenyingcharoen ◽  
Sukunphat Malimas ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Acetic Acid Bacterium ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Multi-Trait Wheat Rhizobacteria from Calcareous Soil with Biocontrol Activity Promote Plant Growth and Mitigate Salinity Stress

2021 ◽  
Vol 9 (8) ◽  
pp. 1588
Author(s):  
Anastasia Venieraki ◽  
Styliani N. Chorianopoulou ◽  
Panagiotis Katinakis ◽  
Dimitris L. Bouranis
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Calcareous Soil ◽  
In Vitro Studies ◽  
Pgp Traits ◽  
Biocontrol Activity ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Microbial Fertilizers

Plant growth promoting rhizobacteria (PGPR) can be functional microbial fertilizers and/or biological control agents, contributing to an eco-spirit and safe solution for chemical replacement. Therefore, we have isolated rhizospheric arylsulfatase (ARS)-producing bacteria, belonging to Pseudomonas and Bacillus genus, from durum wheat crop grown on calcareous soil. These isolates harbouring plant growth promoting (PGP) traits were further evaluated in vitro for additional PGP traits, including indole compounds production and biocontrol activity against phytopathogens, limiting the group of multi-trait strains to eight. The selected bacterial strains were further evaluated for PGP attributes associated with biofilm formation, compatibility, salt tolerance ability and effect on plant growth. In vitro studies demonstrated that the multi-trait isolates, Bacillus (1.SG.7, 5.SG.3) and Pseudomonas (2.SG.20, 2.C.19) strains, enhanced the lateral roots abundance and shoots biomass, mitigated salinity stress, suggesting the utility of beneficial ARS-producing bacteria as potential microbial fertilizers. Furthermore, in vitro studies demonstrated that compatible combinations of multi-trait isolates, Bacillus sp. 1.SG.7 in a mixture coupled with 5.SG.3, and 2.C.19 with 5.SG.3 belonging to Bacillus and Pseudomonas, respectively, may enhance plant growth as compared to single inoculants.

Interactive effect of biochar and plant growth-promoting bacterial endophytes on ameliorating salinity stress in maize

2015 ◽  
Vol 42 (8) ◽  
pp. 770 ◽  
Author(s):  
Saqib Saleem Akhtar ◽  
Mathias Neumann Andersen ◽  
Muhammad Naveed ◽  
Zahir Ahmad Zahir ◽  
Fulai Liu
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Crop Production ◽  
Interactive Effect ◽  
Nutrient Balance ◽  
Bacterial Strains ◽  
Negative Effects ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Salt Affected Soils

The objective of this work was to study the interactive effect of biochar and plant growth-promoting endophytic bacteria containing 1-aminocyclopropane-1-carboxylate deaminase and exopolysaccharide activity on mitigating salinity stress in maize (Zea mays L.). The plants were grown in a greenhouse under controlled conditions, and were subjected to separate or combined treatments of biochar (0% and 5%, w/w) and two endophytic bacterial strains (Burkholderia phytofirmans (PsJN) and Enterobacter sp. (FD17)) and salinity stress. The results indicated that salinity significantly decreased the growth of maize, whereas both biochar and inoculation mitigated the negative effects of salinity on maize performance either by decreasing the xylem Na+ concentration ([Na+]xylem) uptake or by maintaining nutrient balance within the plant, especially when the two treatments were applied in combination. Moreover, in biochar-amended saline soil, strain FD17 performed significantly better than did PsJN in reducing [Na+]xylem. Our results suggested that inoculation of plants with endophytic baterial strains along with biochar amendment could be an effective approach for sustaining crop production in salt-affected soils.

scholarly journals Biochemical and Genetic Bases of Indole-3-Acetic Acid (Auxin Phytohormone) Degradation by the Plant-Growth-Promoting Rhizobacterium Paraburkholderia phytofirmans PsJN

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Raúl Donoso ◽  
Pablo Leiva-Novoa ◽  
Ana Zúñiga ◽  
Tania Timmermann ◽  
Gonzalo Recabarren-Gajardo ◽  
...  
Keyword(s):  
Energy Source ◽  
Gene Regulation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Plant Hormone ◽  
Content Type ◽  
Gene Functions ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

ABSTRACT Several bacteria use the plant hormone indole-3-acetic acid (IAA) as a sole carbon and energy source. A cluster of genes (named iac) encoding IAA degradation has been reported in Pseudomonas putida 1290, but the functions of these genes are not completely understood. The plant-growth-promoting rhizobacterium Paraburkholderia phytofirmans PsJN harbors iac gene homologues in its genome, but with a different gene organization and context than those of P. putida 1290. The iac gene functions enable P. phytofirmans to use IAA as a sole carbon and energy source. Employing a heterologous expression system approach, P. phytofirmans iac genes with previously undescribed functions were associated with specific biochemical steps. In addition, two uncharacterized genes, previously unreported in P. putida and found to be related to major facilitator and tautomerase superfamilies, are involved in removal of an IAA metabolite called dioxindole-3-acetate. Similar to the case in strain 1290, IAA degradation proceeds through catechol as intermediate, which is subsequently degraded by ortho-ring cleavage. A putative two-component regulatory system and a LysR-type regulator, which apparently respond to IAA and dioxindole-3-acetate, respectively, are involved in iac gene regulation in P. phytofirmans. These results provide new insights about unknown gene functions and complex regulatory mechanisms in IAA bacterial catabolism. IMPORTANCE This study describes indole-3-acetic acid (auxin phytohormone) degradation in the well-known betaproteobacterium P. phytofirmans PsJN and comprises a complete description of genes, some of them with previously unreported functions, and the general basis of their gene regulation. This work contributes to the understanding of how beneficial bacteria interact with plants, helping them to grow and/or to resist environmental stresses, through a complex set of molecular signals, in this case through degradation of a highly relevant plant hormone.

Sign in / Sign up

Export Citation Format

Share Document