scholarly journals OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds

2018 ◽  
Vol 38 (3) ◽  
Author(s):  
Wenxin Bian ◽  
Buliang Meng ◽  
Xiaojie Li ◽  
Siyuan Wang ◽  
Xiaoqing Cao ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Antioxidant Activities ◽  
Low Cost ◽  
Human Fibroblasts ◽  
Human Keratinocytes ◽  
Skin Wound ◽  
New Drugs ◽  
Toxic Activity ◽  
Promote Wound Healing

Nowadays, the number of chronic trauma cases caused by a variety of factors such as the world’s population-ageing and chronic diseases is increasing steadily, and thus effective treatment for chronic wounds has become a severe clinical challenge, which also burdens the patient both physically and financially. Therefore, it is urgent to develop new drugs to accelerate the healing of wounds. Bioactive peptides, which are relatively low cost, easy to produce, store and transport, have become an excellent choice. In this research, we identified a novel peptide OA-GL21, with an amino acid sequence of ‘GLLSGHYGRVVSTQSGHYGRG’, from the skin secretions of Odorrana andersonii. Our results showed that OA-GL21 exerted the ability to promote wound healing of human keratinocytes (HaCaT) and human fibroblasts in a dose- and time-denpendent manner. However, OA-GL21 had no significant effect on the proliferation of these two cells. Significantly, OA-GL21 showed obvious ability to promote wound healing in the full-thickness skin wound model in dose- and scar-free manners. Further studies showed that OA-GL21 had no direct antibacterial, hemolytic, and acute toxic activity; it had weak antioxidant activities but high stability. In conclusion, this research proved the promoting effects of OA-GL21 on cellular and animal wounds, and thus provided a new peptide template for the development of wound-repairing drugs.

Accelerated Wound Healing Induced by a Novel Amphibian Peptide (OA-FF10)

2019 ◽  
Vol 26 (4) ◽  
pp. 261-270 ◽  
Author(s):  
Naixin Liu ◽  
Zhe Li ◽  
Buliang Meng ◽  
Wenxin Bian ◽  
Xiaojie Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Low Cost ◽  
Financial Burden ◽  
Human Keratinocytes ◽  
Critical Issue ◽  
Disulfide Bridge ◽  
Toxic Activity

Background: Despite the continued development of modern medicine, chronic wounds are still a critical issue in clinical treatment, placing a great physiological, psychological, and financial burden on patients. Researchers have investigated many methods to solve this problem, with bioactive peptides gaining increasing attention due to their considerable advantages and diverse functions, as well as low cost, simple storage, and easy transportation. Methods: In this research, a novel peptide (named OA-FF10) was identified from the skin secretions of the odorous frog species Odorrana andersonii. The sequence of mature OA-FF10 was “FFTTSCRSGC”, which was produced by the post-translational processing of a 61-residue prepropeptide. Results: Similar to most frog peptides, OA-FF10 showed an intramolecular disulfide bridge at the C-terminus. OA-FF10 demonstrated no antibacterial, antioxidant, hemolytic, or acute toxic activity, but promoted wound healing and proliferation of human keratinocytes (HaCaT) both time- and dose-dependently. Furthermore, while OA-FF10 had no effect on wound healing of Human Skin Fibroblasts (HSF), it did accelerate healing in a full-thickness skin-wound mouse model. Conclusion: Our research revealed the strong wound-healing activity of OA-FF10 in vivo and in vitro, thus providing a new candidate for the development of novel wound-healing drugs.

scholarly journals The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 457
Author(s):  
Andreu Blanquer ◽  
Jana Musilkova ◽  
Elena Filova ◽  
Johanka Taborska ◽  
Eduard Brynda ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Chronic Wounds ◽  
Human Keratinocytes ◽  
Skin Wound ◽  
Platelet Lysate ◽  
Therapeutic Approaches ◽  
Skin Wound Healing ◽  
New Therapeutic Approaches ◽  
Proliferation And Differentiation

Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research of new therapeutic approaches to wound healing includes the development of nanofibrous meshes and the use of platelet lysate (PL) to stimulate skin regeneration. This study considers a combination of a degradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) membranes (NF) and fibrin loaded with various concentrations of PL aimed at the development of bioactive skin wound healing dressings. The cytocompatibility of the NF membranes, as well as the effect of PL, was evaluated in both monocultures and co-cultures of human keratinocytes and human endothelial cells. We determined that the keratinocytes were able to adhere on all the membranes, and their increased proliferation and differentiation was observed on the membranes that contained fibrin with at least 50% of PL (Fbg + PL) after 14 days. With respect to the co-culture experiments, the membranes with fibrin with 20% of PL were observed to enhance the metabolic activity of endothelial cells and their migration, and the proliferation and differentiation of keratinocytes. The results suggest that the newly developed NF combined with fibrin and PL, described in the study, provides a promising dressing for chronic wound healing purposes.

scholarly journals EndogenousN-acyl taurines regulate skin wound healing

2016 ◽  
Vol 113 (30) ◽  
pp. E4397-E4406 ◽  
Author(s):  
Oscar Sasso ◽  
Silvia Pontis ◽  
Andrea Armirotti ◽  
Giorgia Cardinali ◽  
Daniela Kovacs ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Fibroblasts ◽  
Primary Cultures ◽  
Acid Amide ◽  
Human Keratinocytes ◽  
Skin Wound ◽  
Bioactive Molecules ◽  
Transient Receptor Potential Vanilloid ◽  
Skin Wound Healing ◽  
Long Chain

The intracellular serine amidase, fatty acid amide hydrolase (FAAH), degrades a heterogeneous family of lipid-derived bioactive molecules that include amides of long-chain fatty acids with taurine [N-acyl-taurines (NATs)]. The physiological functions of the NATs are unknown. Here we show that genetic or pharmacological disruption of FAAH activity accelerates skin wound healing in mice and stimulates motogenesis of human keratinocytes and differentiation of human fibroblasts in primary cultures. Using untargeted and targeted lipidomics strategies, we identify two long-chain saturated NATs—N-tetracosanoyl-taurine [NAT(24:0)] andN-eicosanoyl-taurine [NAT(20:0)]—as primary substrates for FAAH in mouse skin, and show that the levels of these substances sharply decrease at the margins of a freshly inflicted wound to increase again as healing begins. Additionally, we demonstrate that local administration of synthetic NATs accelerates wound closure in mice and stimulates repair-associated responses in primary cultures of human keratinocytes and fibroblasts, through a mechanism that involves tyrosine phosphorylation of the epidermal growth factor receptor and an increase in intracellular calcium levels, under the permissive control of transient receptor potential vanilloid-1 receptors. The results point to FAAH-regulated NAT signaling as an unprecedented lipid-based mechanism of wound-healing control in mammalian skin, which might be targeted for chronic wound therapy.

scholarly journals Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

2020 ◽  
Author(s):  
Francesca Rossi ◽  
Giada Magni ◽  
Francesca Tatini ◽  
Martina Banchelli ◽  
Federica Cherchi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blue Light ◽  
Chronic Wounds ◽  
Human Fibroblasts ◽  
Healing Process ◽  
Research Area ◽  
Wound Management ◽  
Blue Led ◽  
Led Light ◽  
New Research

In recent years, photobiomodulation (PBM) has been recognized as a physical therapy in wound management. Despite several published research papers, the mechanism underlying photobiomodulation is still not completely understood. The investigation about application of blue light to improve wound healing is a relatively new research area. Tests in selected patients evidenced a stimulation of the healing process in superficial and chronic wounds treated with a blue LED light emitting at 420 nm; a study in animal model pointed out a faster healing process in superficial wound, with an important role of fibroblasts and myofibroblasts. Here we present a study aiming at evidencing the effects of blue light on the proliferation and metabolism in fibroblasts and keratinocytes. Different light doses were used to treat the cells, evidencing inhibitory and stimulatory effects. Electrophysiology was used to investigate the effects on membrane currents, while Raman spectroscopy revealed the mitochondrial Cytochrome C (Cyt C) oxidase dependence on blue light irradiation. In conclusion, we observed that the blue LED light can be used to modulate the activity of human fibroblasts, and the effects in wound healing are particularly evident when studying the fibroblasts and keratinocytes co-cultures.

scholarly journals Formulation and Evaluation of Wound Healing Effect of Aqueous Herbal Cream Containing Various Extracts of Passiflora foetida Linn., Leaves

2019 ◽  
pp. 490-499
Author(s):  
Akila E ◽  
C. Geetha Priya
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Soft Tissues ◽  
Folk Medicine ◽  
New Drugs ◽  
Herbal Remedies ◽  
Control Group ◽  
Wound Model ◽  
Number Of Patients ◽  
Passiflora Foetida

Wounds are a major cause of concern for the patient and clinician alike; chronic Wounds affect a large number of patients and seriously reduce their quality of life. Wound healing is the process of repair that follows injury to the skin and other soft tissues. A cream is a preparation of a medication for topical use that contains a water base. Essentially, it is a preparation of oil in water. Herbal remedies used in folk medicine provide an interesting and still largely unexplored source for the creation and development of potentially new drugs, which might help to overcome the growing problem of resistance and also the toxicity of the currently available commercial antibiotics. In this study we have formulated an Aqueous herbal cream satisfying almost all pharmaceutical parameters which shows better wound healing activity. The wound healing of effect of Aqueous Herbal cream of various extracts of leaves of Passiflora foetida L. was evaluated by incision and excision wound model. The experimental results and histopathological studies showed that Aqueous Herbal Cream of Passiflora foetida L. leaves exhibits significant wound healing property as compared to control group of animals.

scholarly journals EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Letícia Fuganti CAMPOS ◽  
Eliane TAGLIARI ◽  
Thais Andrade Costa CASAGRANDE ◽  
Lúcia de NORONHA ◽  
Antônio Carlos L. CAMPOS ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Type I Collagen ◽  
Chronic Wounds ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Type I ◽  
Collagen Deposition ◽  
Skin Wound Healing

ABSTRACT Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

scholarly journals Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization

2020 ◽  
Vol 8 ◽  
Author(s):  
Pengcheng Xu ◽  
Yaguang Wu ◽  
Lina Zhou ◽  
Zengjun Yang ◽  
Xiaorong Zhang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Platelet Rich Plasma ◽  
Skin Wound ◽  
Local Inflammation ◽  
Skin Wound Healing

Abstract Background Autologous platelet-rich plasma (PRP) has been suggested to be effective for wound healing. However, evidence for its use in patients with acute and chronic wounds remains insufficient. The aims of this study were to comprehensively examine the effectiveness, synergy and possible mechanism of PRP-mediated improvement of acute skin wound repair. Methods Full-thickness wounds were made on the back of C57/BL6 mice. PRP or saline solution as a control was administered to the wound area. Wound healing rate, local inflammation, angiogenesis, re-epithelialization and collagen deposition were measured at days 3, 5, 7 and 14 after skin injury. The biological character of epidermal stem cells (ESCs), which reflect the potential for re-epithelialization, was further evaluated in vitro and in vivo. Results PRP strongly improved skin wound healing, which was associated with regulation of local inflammation, enhancement of angiogenesis and re-epithelialization. PRP treatment significantly reduced the production of inflammatory cytokines interleukin-17A and interleukin-1β. An increase in the local vessel intensity and enhancement of re-epithelialization were also observed in animals with PRP administration and were associated with enhanced secretion of growth factors such as vascular endothelial growth factor and insulin-like growth factor-1. Moreover, PRP treatment ameliorated the survival and activated the migration and proliferation of primary cultured ESCs, and these effects were accompanied by the differentiation of ESCs into adult cells following the changes of CD49f and keratin 10 and keratin 14. Conclusion PRP improved skin wound healing by modulating inflammation and increasing angiogenesis and re-epithelialization. However, the underlying regulatory mechanism needs to be investigated in the future. Our data provide a preliminary theoretical foundation for the clinical administration of PRP in wound healing and skin regeneration.

Tofu-Based Hybrid Hydrogels with Antioxidant and Low Immunogenicity Activity for Enhanced Wound Healing

2019 ◽  
Vol 15 (7) ◽  
pp. 1371-1383 ◽  
Author(s):  
Jun Huang ◽  
Long Chen ◽  
Qijuan Yuan ◽  
Zhipeng Gu ◽  
Jun Wu
Keyword(s):  
Wound Healing ◽  
Free Radicals ◽  
Antioxidant Activities ◽  
Skin Wound ◽  
Hybrid Hydrogel ◽  
Wound Model ◽  
Hybrid Hydrogels ◽  
Thickness Skin ◽  
Regeneration Effect

Free radicals and inflammation in the skin suffering from trauma cause oxidative damage and delayed healing, leading to adverse wound conditions. To adequately investigate the effects of free radicals and controlled immunogenicity for wound healing, we propose a tofu-based hybrid hydrogel with antioxidant and low immunogenicity properties that can be used for wound healing. Tofu, a food source material, was introduced for the first time into gelatin methacryloyl (GelMA) hydrogels by the photo-crosslinking method. The results demonstrated that the incorporation of tofu influenced the pores, swelling, water vapor transmission and compressive properties of hydrogels greatly. The antioxidant activities of hydrogels had been enhanced with increasing rations of tofu, and the fibroblast culture showed good proliferation on the hybrid hydrogels, as well as slight immunogenicity, thereby inducing the M2 differentiation of macrophages. Further, a full-thickness skin wound model was created to study the healing effect of hybrid hydrogels. In vivo results confirmed that the antioxidant activity and slight immunological stimulation properties of tofu hydrogels could accelerate the wound healing rate and improve the skin tissue regeneration effect. The present study validates that the tofu-based hybrid hydrogels have multiple bioactivities and could be potential antioxidant and immunoregulation hydrogels in wound healing applications.

scholarly journals Antioxidant, Anti-Inflammatory and Wound Healing of Biosynthetic Gold Nanoparticles Using Mangosteen (G. Mangostona)

2021 ◽  
Vol 8 (2) ◽  
pp. 59-74
Author(s):  
Abdulkadir Mohammed Noori Jassim ◽  
Gufran Mohammed Shafy ◽  
Mustafa Taha Mohammed ◽  
Safana Ahmed Farhan ◽  
Omar Mohammed Noori
Keyword(s):  
Gold Nanoparticles ◽  
Wound Healing ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Spectroscopic Method ◽  
Radical Scavenging ◽  
New Drugs ◽  
Garcinia Mangostana ◽  
Anti Inflammatory ◽  
Peel Extract

In current research, the synthesis of gold nanoparticles was achieved via reducing of gold ions in aqueous solution with Garcinia mangostana (G. mangostana) peel extract. The optimum concentration of gold (Au) solution, concentration ratio of Au solution and extract, temperature, time and pH, the synthesized AuNPs (G. mangostana-gold nanoparticles) were studied by using UV-Vis, FT-IR, AAS, AFM, SEM and Zitasizer. The absorbance peak is noticed between 535-550 nm via UV-Vis spectroscopic method. The SEM, AFM analysis were proofed the particle as spherical in structure and their size between 15-100nm. Therefore, mechanism of AuNPs synthesis had been suggested. Also, the antibacterial activity was examined using different bacteria as well as free radical scavenging activity was tested using 1, 1-Diphenyl-2-picrylhydrazyl (DPPH). The AuNPs produced through biosynthesized method indicated a much elevated antioxidant activity as compared to peel extract of G. mangostana. Toxicity of the NPs and extract were tested via giving orally dose 50 mg/b.w. to mice. Diagnosis of the data (pathological changes) indicated that the AuNPs was non-toxic. The G. mangostana peel extract and AuNPs synthesized by this extract were converted to a cream and used as a wound healing cream. As a results, the AuNPs exhibited important role in wound healing progression compared to control, which may be attributed to their anti-inflammatory, antibacterial and antioxidant activities. Therefore, this research confirms its important use of AuNPs and can be utilized as promising agents for in the development of new drugs.

scholarly journals Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 637 ◽  
Author(s):  
Svetlana Miroshnichenko ◽  
Valeriia Timofeeva ◽  
Elizaveta Permyakova ◽  
Sergey Ershov ◽  
Philip Kiryukhantsev-Korneev ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Biological Activity ◽  
Photoelectron Spectroscopy ◽  
Platelet Rich Plasma ◽  
Low Cost ◽  
Human Fibroblasts ◽  
Covalent Binding ◽  
Covalent Bonding ◽  
Plasma Modification

Biodegradable nanofibers are extensively employed in different areas of biology and medicine, particularly in tissue engineering. The electrospun polycaprolactone (PCL) nanofibers are attracting growing interest due to their good mechanical properties and a low-cost structure similar to the extracellular matrix. However, the unmodified PCL nanofibers exhibit an inert surface, hindering cell adhesion and negatively affecting their further fate. The employment of PCL nanofibrous scaffolds for wound healing requires a certain modification of the PCL surface. In this work, the morphology of PCL nanofibers is optimized by the careful tuning of electrospinning parameters. It is shown that the modification of the PCL nanofibers with the COOH plasma polymers and the subsequent binding of NH2 groups of protein molecules is a rather simple and technologically accessible procedure allowing the adhesion, early spreading, and growth of human fibroblasts to be boosted. The behavior of fibroblasts on the modified PCL surface was found to be very different when compared to the previously studied cultivation of mesenchymal stem cells on the PCL nanofibrous meshes. It is demonstrated by X-ray photoelectron spectroscopy (XPS) that the freeze–thawed platelet-rich plasma (PRP) immobilization can be performed via covalent and non-covalent bonding and that it does not affect biological activity. The covalently bound components of PRP considerably reduce the fibroblast apoptosis and increase the cell proliferation in comparison to the unmodified PCL nanofibers or the PCL nanofibers with non-covalent bonding of PRP. The reported research findings reveal the potential of PCL matrices for application in tissue engineering, while the plasma modification with COOH groups and their subsequent covalent binding with proteins expand this potential even further. The use of such matrices with covalently immobilized PRP for wound healing leads to prolonged biological activity of the immobilized molecules and protects these biomolecules from the aggressive media of the wound.

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