scholarly journals Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease

2018 ◽  
Vol 38 (1) ◽  
Author(s):  
Muhammad Hussain ◽  
Peng Chen ◽  
Guang Mei ◽  
Yongzhe Li ◽  
Hongwu Du
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Epitope Prediction ◽  
Protein Band ◽  
Annexin A1 ◽  
Amino Acid Sequence Alignment ◽  
Clinical Role ◽  
Behcet's Disease ◽  
Healthy Control

The autoantibodies profile of Behçet’s disease (BD) is yet incompletely understood. Annexins are a family of highly conserved proteins which are involved in some human autoimmune diseases. Autoantibodies directed toward Annexin A1 and A2 are involved in BD pathology, but correlation in their clinical role is controversial. The aim of our study is to estimate and evaluate the expression correlation between Annexin A1 and A2 autoantibodies in BD patients. We have designed and implemented different technical approaches to prove the hypothesis. First, bioinformatics tools such as amino acid sequence alignment, epitope prediction analysis, and 3D structural comparison were performed to find out the correlation between Annexin A1 and A2. Second, amplification of the corresponding gene by RT-PCR, then cloning, and purification techniques were applied to acquire the recombinant Annexin A1. Third, the target protein band was excised from gel electrophoresis, digested with trypsin, and analyzed by MALDI-TOF/TOF. Finally, in-house ELISA was developed to determine the induced anti-Annexin A1 autoantibodies in BD patients. Obtained results demonstrated that the BD serum reactivity against recombinant Annexin A1 was significantly higher as compared with healthy control (HC) (P<0.001). Moreover, bioassay results of Annexin A1 and A2 also showed the presence, absence, and independent coexistence of autoantibodies, when reacted with BD sera. In conclusion, Annexin A1 has a similar immunogenic expression and correlation with its analog Annexin A2 and their association may be a novel immune target of BD in Han Chinese population.

scholarly journals The Correlation of CD206, CD209, and Disease Severity in Behçet’s Disease with Arthritis

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Bunsoon Choi ◽  
Chang-Hee Suh ◽  
Hyoun-Ah Kim ◽  
Hasan M. Sayeed ◽  
Seonghyang Sohn
Keyword(s):  
Pattern Recognition ◽  
Disease Severity ◽  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Pattern Recognition Receptors ◽  
Behcet's Disease ◽  
Significant Difference ◽  
Cytokine Levels ◽  
Healthy Control

The purpose of this study was to clarify the role of pattern recognition receptors in Behçet’s disease (BD). The frequencies of several pattern recognition receptors (CD11b, CD11c, CD32, CD206, CD209, and dectin-1) were analyzed in patients with BD by flow cytometry, and cytokine levels, interleukin- (IL-) 18, IL-23, and IL-17A, were compared in plasma. The analysis was performed in active (n=13) and inactive (n=13) stages of BD patients. Rheumatoid arthritis patients (n=19), as a disease control, and healthy control (HC) (n=19) were enrolled. The frequencies of CD11b+ and CD32+ cells were significantly increased in active BD patients compared to HC. Disease severity score was correlated to CD11c+, CD206+, and CD209+ in whole leukocytes and CD11b+, CD11c+, CD206+, CD209+, and Dectin-1+ in granulocytes. The plasma levels of IL-17A were significantly different between HC and active BD. IL-18 showed significant difference between active and inactive BD patients. From this study, we concluded the expressions of several pattern recognition receptors were correlated to the joint symptoms of BD.

scholarly journals Detection of Anti-Chlamydia pneumonia IgG and Anti-HSV-1 IgG antibodies in sample of Iraqi Behcet's disease patients

2014 ◽  
Vol 8 (2) ◽  
pp. 34-36
Author(s):  
Dunya fareed Salloom ◽  
Dhuha Saad Saleh ◽  
Jassem Tuama Al-Khafaji
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Igg Antibody ◽  
Herpes Simplex Virus 1 ◽  
Behcet's Disease ◽  
Causative Agents ◽  
Healthy Control ◽  
Simplex Virus ◽  
Hsv 1

Behcet's disease (BD) is a chronic inflammation with characterized lesion, including oral ulcer, skin lesion, genital lesion and ocular manifestation. It affects both genders. The etiologies of BD is unknown genetic, bacterial and viral have proposed as causative agents, for that reason the aim of this study to detect Anti-Herpes Simplex Virus-1(HSV-1) IgG antibody and Anti-Chlamydia pneumonia IgG antibody by ELISA in sera of seventy Iraqi patients and twenty Iraqi healthy control. 34.2% of patients have Anti-HSV-1 IgG antibody while 21.4% of patients have Anti-Chlamydia pneumonia IgG antibody, this lead to suggest that HSV-1 and Chlamydia pneumonia may be one of the etiological factor in BD.

Coagulation and Fibrinolytic Activity in Behçet's Disease

1991 ◽  
Vol 66 (03) ◽  
pp. 292-294 ◽  
Author(s):  
K K Hampton ◽  
M A Chamberlain ◽  
D K Menon ◽  
J A Davies
Keyword(s):  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Behçet’S Disease ◽  
Fibrinolytic Activity ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Plasminogen Activator Inhibitor ◽  
Behcet's Disease ◽  
Tissue Plasminogen ◽  
Von Willebrand

SummaryCoagulation and fibrinolytic activities were studied in 18 subjects with Behçet's disease and compared with results from 14 matched control patients suffering from sero-negative arthritis. Significantly higher plasma concentrations (median and range) were found in Behçet's patients for the following variables: fibrinogen 3.7 (1.7-6.9) vs 3.0 (2.0-5.1) g/1, p <0.05; von Willebrand factor antigen, 115 (72-344) vs 74 (60-119)%, p <0.002; plasminogen activator activity (106/ECLT2) 219 (94-329) vs 137 (78-197) units, p <0.002; tissue plasminogen activator inhibitor (t-PA-I) activity, 9.1 (5.5-19.3) vs 5.1 (1.8-12.0) IU/ml, p <0.002; and PAI-1 antigen, 13.9 (4.5-20.9) vs 6.4 (2.4-11.1) ng/ml, p <0.002. Protein C antigen was significantly lower: 97 (70-183) vs 126 (96-220)%, p <0.02. No differences were observed in antithrombin III activity or antigen, factor VIII coagulant activity, fibrinopeptides A and Bβ15-42, plasminogen, α-2-antiplasmin, functional and immunological tissue-plasminogen activator, thrombin-antithrombin complexes and D-dimer. Levels of tissue plasminogen activator inhibitor (activity and antigen) correlated with disease activity while fibrinogen and von Willebrand factor concentrations did not. Seven of the 18 subjects with Behçet's disease had suffered thrombotic events but it was not possible to distinguish these from the 11 patients without thrombosis using the assays performed. The results suggest the abnormal fibrinolytic activity in Behçet's disease is due to increased inhibition of tissue plasminogen activator. No abnormality of coagulation or fibrinolytic activity specific to Behçet's disease was detected.

Circulating Thrombomodulin as a Clue of Endothelial Damage in Behçet’s Disease

1996 ◽  
Vol 75 (06) ◽  
pp. 974-975 ◽  
Author(s):  
İbrahim C Haznedaroğlu ◽  
Oktay Özdemir ◽  
Osman Özcebe ◽  
Semra V Dündar ◽  
Şerafettin Kirazli
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Endothelial Damage ◽  
Behcet's Disease

Radiological evaluation of vasculo-Behcet's disease

1993 ◽  
Vol 29 (1) ◽  
pp. 62 ◽  
Author(s):  
Kyung Hwan Lee ◽  
Jae Hyung Park ◽  
Joon Ku Han ◽  
Hyun Ae Park ◽  
Jin Wook Chung ◽  
...  
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Radiological Evaluation ◽  
Behcet's Disease

Subacute thyroiditis in association with psoriasis and behcet's disease

2018 ◽  
Author(s):  
Ferreira Paulo Carvalho ◽  
Bruno Maia ◽  
Antonio Marinho ◽  
Ana Veloso ◽  
Ivo Cunha ◽  
...  
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Subacute Thyroiditis ◽  
Behcet's Disease

Endovascular Aneurysm Exclusion Along a Femorodistal Venous Bypass in Active Behçet's Disease

2002 ◽  
Vol 9 (5) ◽  
pp. 694-698 ◽  
Author(s):  
Silvia B. Gretener ◽  
Dai-Do Do ◽  
Iris Baumgartner ◽  
Hans-Peter Dinkel ◽  
Jürg Schmidli ◽  
...  
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Venous Bypass ◽  
Behcet's Disease

Behçet's Disease and Trace Elements

1975 ◽  
Vol 37 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Shigeo NISIHYAMA ◽  
Yoshiaki HORI ◽  
Michitoshi MURAKAMI ◽  
Shichiro MIYAZAWA ◽  
Mitsuko OHTA ◽  
...  
Keyword(s):  
Trace Elements ◽  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Behcet's Disease
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