scholarly journals Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro

2016 ◽  
Vol 36 (6) ◽  
Author(s):  
Bohan Cheng ◽  
Mengqi Wu ◽  
Songsong Xu ◽  
Xinyang Zhang ◽  
Yuxiang Wang ◽  
...  
Keyword(s):  
Fatty Acid Binding Protein ◽  
Peroxisome Proliferator ◽  
Induction Method ◽  
Preadipocyte Differentiation ◽  
Peroxisome Proliferator Activated Receptor ◽  
Fatty Acid Binding ◽  
Gpdh Activity ◽  
Acid Binding Protein ◽  
Glycerol 3 Phosphate Dehydrogenase

Chicken preadipocytes cultured in cocktail supplement with rosiglitazone resulted in a marked increase in lipid droplet accumulation, glycerol-3-phosphate dehydrogenase (GPDH) activity and mRNA expression of adipocyte fatty acid-binding protein (aP2), G0/G1 switch gene 2 (G0S2), peroxisome proliferator-activated receptor γ (PPARγ) and lipolysis. The present study provides a novel induction method for in vitro chicken preadipocyte differentiation.

scholarly journals Traditional Herbal Formula Oyaksungi-San Inhibits Adipogenesis in 3T3-L1 Adipocytes

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Sae-Rom Yoo ◽  
Chang-Seob Seo ◽  
Hyeun-Kyoo Shin ◽  
Soo-Jin Jeong
Keyword(s):  
Binding Protein ◽  
Peroxisome Proliferator ◽  
Herbal Formula ◽  
Related Factors ◽  
Peroxisome Proliferator Activated Receptor ◽  
Fatty Acid Binding ◽  
Gpdh Activity ◽  
Protein Levels ◽  
Intracellular Lipid Accumulation ◽  
Glycerol 3 Phosphate Dehydrogenase

Background. Oyaksungi-san (OYSGS) is a herbal formula that has been used for treating cardiovascular diseases in traditional Asian medicine. Here, we investigated the antiadipogenic effect of OYSGS extract in 3T3-L1 adipose cells.Methods. 3T3-L1 preadipocytes were differentiated into adipocytes with or without OYSGS. After differentiation, we measured Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, leptin production, mRNA, and protein levels of adipogenesis-related factors.Results. OYSGS extract dramatically inhibited intracellular lipid accumulation in the differentiated adipocytes. It also significantly suppressed the (GPDH) activity, triglyceride (TG) content, and leptin production by reducing the expression of adipogenesis-related genes including lipoprotein lipase, fatty acid binding protein 4, CCAAT/enhancer-binding protein-alpha (C/EBP-α), and peroxisome proliferator-activated receptor gamma (PPAR-γ). Furthermore, OYSGS clearly enhanced phosphorylation of AMP-activated protein kinase (AMPK) as well as its substrate acetyl CoA (ACC) carboxylase.Conclusions. Our results demonstrate that OYSGS negatively controls TG accumulation in 3T3-L1 adipocytes. We suggest antiadipogenic activity of OYSGS and its potential benefit in preventing obesity.

IL-6 cooperates with peroxisome proliferator-activated receptor-α-ligands to induce liver fatty acid binding protein (LFABP) up-regulation

2013 ◽  
Vol 33 (7) ◽  
pp. 1019-1028 ◽  
Author(s):  
Margarita Vida ◽  
Antonia Serrano ◽  
Miguel Romero-Cuevas ◽  
Francisco J. Pavón ◽  
Águeda González-Rodriguez ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Peroxisome Proliferator ◽  
Liver Fatty Acid ◽  
Peroxisome Proliferator Activated Receptor ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Glucose regulates fatty acid binding protein interaction with lipids and peroxisome proliferator-activated receptor α

2010 ◽  
Vol 51 (11) ◽  
pp. 3103-3116 ◽  
Author(s):  
Heather A. Hostetler ◽  
Madhumitha Balanarasimha ◽  
Huan Huang ◽  
Matthew S. Kelzer ◽  
Alagammai Kaliappan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Protein Interaction ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Fatty Acid Binding ◽  
Acid Binding Protein
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