scholarly journals Structural characterization of the late competence protein ComFB from Bacillus subtilis

2015 ◽  
Vol 35 (2) ◽  
Author(s):  
Tatyana A. Sysoeva ◽  
Lukas B. Bane ◽  
Daphne Y. Xiao ◽  
Baundauna Bose ◽  
Scott S. Chilton ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Structural Characterization ◽  
Competence Development ◽  
Regulatory Role ◽  
In Vivo Studies ◽  
Genetic Competence

Development of genetic competence in Bacillus subtilis results in expression of late competence genes, including comFB. In vitro and in vivo studies revealed that ComFB dimerizes, binds zinc, and possibly plays a regulatory role in competence development.

Development and characterization of highly selective target-sensitive liposomes for the delivery of streptokinase: in vitro/in vivo studies

Drug Delivery ◽  
2014 ◽  
Vol 23 (3) ◽  
pp. 791-797 ◽  
Author(s):  
Bhuvaneshwar Vaidya ◽  
Manasa K. Nayak ◽  
Debabrata Dash ◽  
Govind P. Agrawal ◽  
Suresh P. Vyas
Keyword(s):  
In Vivo Studies ◽  
Selective Target

Characterization of nociceptin receptors in the periphery: in vitro and in vivo studies

1999 ◽  
Vol 359 (3) ◽  
pp. 160-167 ◽  
Author(s):  
Raffaella Bigoni ◽  
Sandro Giuliani ◽  
G. Calo’ ◽  
Anna Rizzi ◽  
Remo Guerrini ◽  
...  
Keyword(s):  
In Vivo Studies

scholarly journals ENHANCED FLUORESCENCE IMAGING WITH DMSO-MEDIATED OPTICAL CLEARING

2010 ◽  
Vol 03 (03) ◽  
pp. 153-158 ◽  
Author(s):  
SANJEEV KARMA ◽  
JAMES HOMAN ◽  
CHARLES STOIANOVICI ◽  
BERNARD CHOI
Keyword(s):  
Fluorescence Emission ◽  
Model Systems ◽  
In Vivo Studies ◽  
In Vivo Model ◽  
Fluorescence Signal ◽  
Optical Clearing ◽  
Treated Site

Recent studies have demonstrated that topical application of glycerol on intact skin does not affect its optical scattering properties. Investigators from our research group recently revisited the use of dimethyl sulfoxide (DMSO) as an agent with optical clearing potential. We address the use of optical clearing to enhance quantitation of subsurface fluorescence emission. We employed both in vitro and in vivo model systems to study the effect of topical DMSO application on fluorescence emission. Our in vitro experiments performed on a tissue-simulating phantom suggest that DMSO-mediated optical clearing enables enhanced characterization of subsurface fluorophores. With topical DMSO application, a marked increase in fluorescence emission was observed. After 30 min, the fluorescence signal at the DMSO-treated site was 9× greater than the contralateral saline-treated site. This ratio increased to 13× at 105 min after agent application. In summary, DMSO is an effective optical clearing agent for improved fluorescence emission quantitation and warrants further study in preclinical in vivo studies. Based on outcomes from previous clinical studies on the toxicity profile of DMSO, we postulate that clinical application of DMSO as an optical clearing agent, can be performed safely, although further study is warranted.

Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE): Biochemical Features and Therapeutic Approaches

2007 ◽  
Vol 27 (1-3) ◽  
pp. 151-163 ◽  
Author(s):  
M. C. Lara ◽  
M. L. Valentino ◽  
J. Torres-Torronteras ◽  
M. Hirano ◽  
R. Martí
Keyword(s):  
Molecular Genetic ◽  
In Vivo Studies ◽  
Gene Encoding ◽  
Mitochondrial Neurogastrointestinal Encephalomyopathy ◽  
Mtdna Replication ◽  
Biochemical Features

Over the last 15 years, important research has expanded our knowledge of the clinical, molecular genetic, and biochemical features of mitochondrial neurogastrointestinal encephalomyopathy (MNGIE). The characterization of mitochondrial involvement in this disorder and the seminal determination of its genetic cause, have opened new possibilities for more detailed and deeper studies on the pathomechanisms in this progressive and fatal disease. It has been established that MNGIE is caused by mutations in the gene encoding thymidine phosphorylase (TP), which lead to absolute or nearly complete loss of its catalytic activity, producing systemic accumulations of its substrates, thymidine (dThd) and deoxyuridine (dUrd). Findings obtained from in vitro and in vivo studies indicate that the biochemical imbalances specifically impair mitochondrial DNA (mtDNA) replication, repair, or both leading to mitochondrial dysfunction. We have proposed that therapy for MNGIE should be aimed at reducing the concentrations of these toxic nucleosides to normal or nearly normal levels. The first treatment, allogeneic stem-cell transplantation (alloSCT) reported in 2006, produced a nearly full biochemical correction of the dThd and dUrd imbalances in blood. Clinical follow-up of this and other patients receiving alloSCT is necessary to determine whether this and other therapies based on a permanent restoration of TP will be effective treatment for MNGIE.

scholarly journals 432. Characterization of a Novel Oncolytic Measles Virus Retargeted Against the Urokinase Receptor: In Vitro and In Vivo Studies

2006 ◽  
Vol 13 ◽  
pp. S166-S167
Author(s):  
Jaime R. Merchan ◽  
Caili Tong ◽  
Takafumi Nakamura ◽  
Ianko Iankov ◽  
Stephen J. Russell
Keyword(s):  
Measles Virus ◽  
Urokinase Receptor ◽  
In Vivo Studies
Sign in / Sign up

Export Citation Format

Share Document