Identification of novel candidate phosphatidic acid-binding proteins involved in the salt-stress response of Arabidopsis thaliana roots

2013 ◽  
Vol 451 (2) ◽  
pp. 343-343 ◽  
Author(s):  
F. Mcloughlin ◽  
S. A. Arisz ◽  
H. L. Dekker ◽  
G. Kramer ◽  
C. G. de Koster ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Response ◽  
Phosphatidic Acid ◽  
Binding Proteins ◽  
Salt Stress Response

Identification of novel candidate phosphatidic acid-binding proteins involved in the salt-stress response of Arabidopsis thaliana roots

2013 ◽  
Vol 450 (3) ◽  
pp. 573-581 ◽  
Author(s):  
Fionn McLoughlin ◽  
Steven A. Arisz ◽  
Henk L. Dekker ◽  
Gertjan Kramer ◽  
Chris G. de Koster ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Phosphatidic Acid ◽  
Stress Responses ◽  
Ribosomal Proteins ◽  
Binding Proteins ◽  
Affinity Purification ◽  
Lipid Binding ◽  
Biotic And Abiotic Stress ◽  
Salt Stress Response

PA (phosphatidic acid) is a lipid second messenger involved in an array of processes occurring during a plant's life cycle. These include development, metabolism, and both biotic and abiotic stress responses. PA levels increase in response to salt, but little is known about its function in the earliest responses to salt stress. In the present study we have combined an approach to isolate peripheral membrane proteins of Arabidopsis thaliana roots with lipid-affinity purification, to identify putative proteins that interact with PA and are recruited to the membrane in response to salt stress. Of the 42 putative PA-binding proteins identified by MS, a set of eight new candidate PA-binding proteins accumulated at the membrane fraction after 7 min of salt stress. Among these were CHC (clathrin heavy chain) isoforms, ANTH (AP180 N-terminal homology) domain clathrin-assembly proteins, a putative regulator of potassium transport, two ribosomal proteins, GAPDH (glyceraldehyde 3-phosphate dehydrogenase) and a PI (phosphatidylinositol) 4-kinase. PA binding and salt-induced membrane recruitment of GAPDH and CHC were confirmed by Western blot analysis of the cellular fractions. In conclusion, the approach of the present study is an effective way to isolate biologically relevant lipid-binding proteins and provides new leads in the study of PA-mediated salt-stress responses in roots.

scholarly journals Phosphatidic acid mediates salt stress response by regulation of MPK6 in Arabidopsis thaliana

2010 ◽  
Vol 188 (3) ◽  
pp. 762-773 ◽  
Author(s):  
Lijuan Yu ◽  
Jianing Nie ◽  
Chunyan Cao ◽  
Yakang Jin ◽  
Min Yan ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Response ◽  
Phosphatidic Acid ◽  
Salt Stress Response

The Structure of the Arabidopsis thaliana SOS3: Molecular Mechanism of Sensing Calcium for Salt Stress Response

2005 ◽  
Vol 345 (5) ◽  
pp. 1253-1264 ◽  
Author(s):  
María José Sánchez-Barrena ◽  
Martín Martínez-Ripoll ◽  
Jian-Kang Zhu ◽  
Armando Albert
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Response ◽  
Molecular Mechanism ◽  
Salt Stress Response

AtPHB2 regulates salt stress response in Arabidopsis thaliana

2021 ◽  
Vol 94 (1) ◽  
pp. 23-32
Author(s):  
Xu Chang ◽  
Guoqing Zhu ◽  
Shiya Chen ◽  
Dan Sun ◽  
Hao He ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Response ◽  
Salt Stress Response

Polyamines and salt stress response and tolerance in Arabidopsis thaliana flowers

2008 ◽  
Vol 46 (5-6) ◽  
pp. 607-613 ◽  
Author(s):  
Annalisa Tassoni ◽  
Marina Franceschetti ◽  
Nello Bagni
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Response ◽  
Salt Stress Response

scholarly journals The GSK3-like Kinase BIN2 Is a Molecular Switch between the Salt Stress Response and Growth Recovery in Arabidopsis thaliana

2020 ◽  
Vol 55 (3) ◽  
pp. 367-380.e6 ◽  
Author(s):  
Jianfang Li ◽  
Huapeng Zhou ◽  
Yan Zhang ◽  
Zhen Li ◽  
Yongqing Yang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Response ◽  
Molecular Switch ◽  
Salt Stress Response ◽  
Growth Recovery
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