scholarly journals Biochemical characterization of the initial steps of the Kennedy pathway in Trypanosoma brucei: the ethanolamine and choline kinases

2009 ◽  
Vol 422 (3) ◽  
pp. 571-571
Author(s):  
F. Gibellini ◽  
W.N. Hunter ◽  
T.K. Smith
Keyword(s):  
Trypanosoma Brucei ◽  
Biochemical Characterization ◽  
Kennedy Pathway

Biochemical characterization of stage-specific isoforms of aspartate aminotransferases from Trypanosoma cruzi and Trypanosoma brucei

2008 ◽  
Vol 161 (1) ◽  
pp. 12-20 ◽  
Author(s):  
Daniela Marciano ◽  
Constanza Llorente ◽  
Dante A. Maugeri ◽  
Candelaria de la Fuente ◽  
Fred Opperdoes ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Trypanosoma Brucei ◽  
Biochemical Characterization

Biochemical characterization of Trypanosoma brucei RNA polymerase II

2006 ◽  
Vol 150 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Anish Das ◽  
Hong Li ◽  
Tong Liu ◽  
Vivian Bellofatto
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Trypanosoma Brucei ◽  
Biochemical Characterization

Trypanosoma brucei brucei: Biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities

2007 ◽  
Vol 115 (4) ◽  
pp. 315-323 ◽  
Author(s):  
Milane de Souza Leite ◽  
Rachel Thomaz ◽  
Fábio Vasconcelos Fonseca ◽  
Rogério Panizzutti ◽  
Anibal E. Vercesi ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Biochemical Characterization ◽  
Nucleoside Triphosphate ◽  
Trypanosoma Brucei Brucei ◽  
Nucleoside Triphosphate Diphosphohydrolase

scholarly journals Biochemical characterization of the initial steps of the Kennedy pathway in Trypanosoma brucei: the ethanolamine and choline kinases

2008 ◽  
Vol 415 (1) ◽  
pp. 135-144 ◽  
Author(s):  
Federica Gibellini ◽  
William N. Hunter ◽  
Terry K. Smith
Keyword(s):  
Trypanosoma Brucei ◽  
Biochemical Characterization ◽  
De Novo ◽  
Carbon Chain ◽  
Surface Proteins ◽  
Membrane Phospholipids ◽  
Ethanolamine Kinase ◽  
Kennedy Pathway ◽  
Catalytically Active ◽  
Membrane Attachment

Ethanolamine and choline are major components of the trypanosome membrane phospholipids, in the form of GPEtn (glycerophosphoethanolamine) and GPCho (glycerophosphocholine). Ethanolamine is also found as an integral component of the GPI (glycosylphosphatidylinositol) anchor that is required for membrane attachment of cell-surface proteins, most notably the variant-surface glycoproteins. The de novo synthesis of GPEtn and GPCho starts with the generation of phosphoethanolamine and phosphocholine by ethanolamine and choline kinases via the Kennedy pathway. Database mining revealed two putative C/EKs (choline/ethanolamine kinases) in the Trypanosoma brucei genome, which were cloned, overexpressed, purified and characterized. TbEK1 (T. brucei ethanolamine kinase 1) was shown to be catalytically active as an ethanolamine-specific kinase, i.e. it had no choline kinase activity. The Km values for ethanolamine and ATP were found to be 18.4±0.9 and 219±29 μM respectively. TbC/EK2 (T. brucei choline/ethanolamine kinase 2), on the other hand, was found to be able to phosphorylate both ethanolamine and choline, even though choline was the preferred substrate, with a Km 80 times lower than that of ethanolamine. The Km values for choline, ethanolamine and ATP were 31.4±2.6 μM, 2.56±0.31 mM and 20.6±1.96 μM respectively. Further substrate specificity analysis revealed that both TbEK1 and TbC/EK2 were able to tolerate various modifications at the amino group, with the exception of a quaternary amine for TbEK1 (choline) and a primary amine for TbC/EK2 (ethanolamine). Both enzymes recognized analogues with substituents on C-2, but substitutions on C-1 and elongations of the carbon chain were not well tolerated.

scholarly journals Structural and Biochemical Characterization of Poly-ADP-ribose Polymerase from Trypanosoma brucei

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Teemu Haikarainen ◽  
Mariana Schlesinger ◽  
Ezeogo Obaji ◽  
Silvia H. Fernández Villamil ◽  
Lari Lehtiö
Keyword(s):  
Trypanosoma Brucei ◽  
Biochemical Characterization

Biochemical characterization of highly active Trypanosoma brucei gambiense glycerol kinase, a promising drug target

2013 ◽  
Vol 154 (1) ◽  
pp. 77-84 ◽  
Author(s):  
E. O. Balogun ◽  
D. K. Inaoka ◽  
T. Shiba ◽  
Y. Kido ◽  
T. Nara ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Drug Target ◽  
Biochemical Characterization ◽  
Glycerol Kinase ◽  
Trypanosoma Brucei Gambiense ◽  
Highly Active ◽  
Kinase A

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

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