scholarly journals Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis

2001 ◽  
Vol 358 (3) ◽  
pp. 791-792 ◽  
Author(s):  
T. H. P. TAN ◽  
S. A. V. EDGERTON ◽  
R. KUMARI ◽  
M. S. B. McALISTER ◽  
S. M. ROWE ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Parasitic Nematode ◽  
Trichinella Spiralis ◽  
Inhibitory Factor ◽  
Macrophage Migration

scholarly journals Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis

2001 ◽  
Vol 357 (2) ◽  
pp. 373 ◽  
Author(s):  
Timothy H.P. TAN ◽  
Steve A.V. EDGERTON ◽  
Rashmi KUMARI ◽  
Mark S.B. McALISTER ◽  
S. Mark ROWE ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Parasitic Nematode ◽  
Trichinella Spiralis ◽  
Inhibitory Factor ◽  
Macrophage Migration

Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis

2001 ◽  
Vol 357 (2) ◽  
pp. 373-383 ◽  
Author(s):  
Timothy H. P. TAN ◽  
Steve A. V. EDGERTON ◽  
Rashmi KUMARI ◽  
Mark S. B. McALISTER ◽  
S. Mark ROWE ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Mononuclear Cells ◽  
Quaternary Structure ◽  
Trichinella Spiralis ◽  
Human Peripheral Blood ◽  
Inhibitory Factor ◽  
Macrophage Migration ◽  
Filarial Nematodes

cDNAs were obtained for macrophage migration-inhibitory factor (MIF)/l-dopachrome methyl ester tautomerase homologues from the parasitic nematodes Trichinella spiralis (TsMIF) and Trichuris trichiura (TtMIF). The translated sequences, which were partly confirmed by sequencing of proteolytic fragments, show 42 and 44% identity respectively with human or mouse MIF, and are shorter by one C-terminal residue. Unlike vertebrate MIF and MIF homologues of filarial nematodes, neither TsMIF nor TtMIF contain cysteine residues. Soluble recombinant TsMIF, expressed in Escherichia coli showed secondary structure (by CD spectroscopy) and quaternary structure (by light-scattering and gel filtration) similar to that of the trimeric mammalian MIFs and d-dopachrome tautomerase. The catalytic specificity of recombinant TsMIF in the ketonization of phenylpyruvate (1.4×106M−1·s−1) was comparable with that of human MIF, while that of p-hydroxyphenylpyruvate (9.1×104M−1·s−1) was 71-fold lower. TsMIF showed high specificity in tautomerization of the methyl ester of l-dopachrome compared with non-esterified l-dopachrome (>87000-fold) and a high kcat (≈ 4×104s−1). The crystal structure, determined to 1.65 Å (1 Å = 0.1nm), was generally similar to that of human MIF, but differed in the boundaries of the putative active-site pocket, which can explain the low activity towards p-hydroxyphenylpyruvate. The central pore was blocked, but was continuous, with the three putative tautomerase sites. Recombinant TsMIF (5ng/ml–5pg/ml) inhibited migration of human peripheral-blood mononuclear cells in a manner similar to that shown by human MIF, but had no effect from 5 to 500ng/ml on anti-CD3-stimulated murine T-cell proliferation. TsMIF was detected in supernatants of T. spiralis larvae cultured in vitro at 6ng/ml (55ng/mg total secreted protein). In conclusion TsMIF has structural, catalytic and cell-migration-inhibitory properties which indicate that it is partially orthologous to mammalian MIF.

scholarly journals Homologues of Human Macrophage Migration Inhibitory Factor from a Parasitic Nematode

2002 ◽  
Vol 277 (46) ◽  
pp. 44261-44267 ◽  
Author(s):  
Xingxing Zang ◽  
Paul Taylor ◽  
Ji Ming Wang ◽  
David J. Meyer ◽  
Alan L. Scott ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Parasitic Nematode ◽  
Inhibitory Factor ◽  
Human Macrophage ◽  
Macrophage Migration

287: Macrophage Migration Inhibitory Factor and its Receptor CD74 are Upregulated in the Urothelium of Interstitial Cystitis Patients

2006 ◽  
Vol 175 (4S) ◽  
pp. 95-96 ◽  
Author(s):  
Pedro L. Vera ◽  
Kenneth A. lczkowski ◽  
Robert M. Moldwin ◽  
Leslie Kushner ◽  
Katherine L. Meyer-Siegler
Keyword(s):  
Interstitial Cystitis ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Macrophage Migration
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