Intracellular ascorbic acid enhances the DNA single-strand breakage and toxicity induced by peroxynitrite in U937 cells

2001 ◽  
Vol 356 (2) ◽  
pp. 509-513 ◽  
Author(s):  
Andrea GUIDARELLI ◽  
Roberta de SANCTIS ◽  
Barbara CELLINI ◽  
Mara FIORANI ◽  
Marina DACHÀ ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Dehydroascorbic Acid ◽  
Ascorbic Acid Content ◽  
Single Strand ◽  
Reduced Form ◽  
U937 Cells ◽  
Intracellular Content ◽  
Independent Mechanism ◽  
Strand Breakage

A well-established protocol to increase the intracellular content of ascorbic acid was used to investigate the effects of the vitamin on DNA single-strand breakage and toxicity mediated by authentic peroxynitrite (ONOO−) in U937 cells. This protocol involved exposure for 60min to 100μM dehydroascorbic acid, which was taken up by the cells and converted into ascorbic acid via a GSH-independent mechanism. At the time of exposure to ONOO−, which was performed in fresh saline immediately after loading with dehydroascorbic acid, the vitamin present in the cells was all in its reduced form. It was found that, in cells that are otherwise ascorbate-deficient, an increase in their ascorbic acid content does not prevent, but rather enhances, the DNA-damaging and lethal responses mediated by exogenous ONOO−. These results therefore suggest that acute supplementation of ascorbic acid can be detrimental for individuals with pathologies associated with a decrease in ascorbic acid and in which ONOO− is known to promote deleterious effects.

scholarly journals Intracellular ascorbic acid enhances the DNA single-strand breakage and toxicity induced by peroxynitrite in U937 cells

2001 ◽  
Vol 356 (2) ◽  
pp. 509 ◽  
Author(s):  
Andrea GUIDARELLI ◽  
Roberta DE SANCTIS ◽  
Barbara CELLINI ◽  
Mara FIORANI ◽  
Marina DACHÀ ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Single Strand ◽  
U937 Cells ◽  
Strand Breakage

scholarly journals INCREASE IN ENZYME ACTIVITIES RELATED TO ASCORBATE AND GLUTATHIONE METABOLISM DURING THIDIAZURON-INDUCED BUD BREAK OF APPLE.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 690b-690
Author(s):  
S. Y. Wang ◽  
H. J. Jiao ◽  
M. Faust
Keyword(s):  
Ascorbic Acid ◽  
Dehydroascorbic Acid ◽  
Ascorbic Acid Content ◽  
Rapid Expansion ◽  
Reduced Form ◽  
Ascorbate Oxidase ◽  
Glutathione Metabolism ◽  
Bud Break ◽  
Protein Thiol ◽  
Ascorbate Free Radical

An increase in ascorbic acid, reduced form of glutathione (GSH), total glutathione, total non-protein thiol (NPSH) and non-glutathione thiol (RSH) occurred as a result of induction by thidiazuron during bud break, whereas dehydroascorbic acid and oxidized glutathione (GSSG) decreased during the same period. Thidiazuron also enhanced the ratio of GSH/GSSG, and activities of catalase, superoxide dismutase (SOD), ascorbate free radical reductase (AFR), ascorbate peroxidase (POD), dehydroascorbate reductase (DHAR), ascorbate oxidase (AAO), and glutathione reductase (GR). The ascorbic acid content and the activities of catalase, SOD, AFR, POD, AAO, and DHAR peaked when buds were in the side green or green tip stage just prior to the start of rapid expansion, and declined thereafter. The GSH, NPSH, RSH, ratio of GSH/GSSG, and activity of GR increased steadily during bud development.

THE SEASONAL VARIATION IN THE ASCORBIC ACID CONTENT OF EDIBLE WILD PLANTS COMMONLY FOUND IN NEW BRUNSWICK

1947 ◽  
Vol 25c (3) ◽  
pp. 95-101 ◽  
Author(s):  
Elizabeth A. Baird ◽  
Muriel G. Lane
Keyword(s):  
Ascorbic Acid ◽  
Seasonal Variation ◽  
New Brunswick ◽  
Acid Content ◽  
Dehydroascorbic Acid ◽  
Ascorbic Acid Content ◽  
Wild Plants ◽  
Edible Wild Plants ◽  
Green Leaves

The ascorbic acid content in the leaves of 10 wild plants that grow in New Brunswick was determined at approximately two-week intervals from June to September. 'Free' and 'total' dehydroascorbic acid were measured, using the method of Roe. The total ascorbic acid is highest in young green leaves. After the plants have flowered the ascorbic acid decreases. It becomes almost negligible when the leaves have wilted and turned brown. 'Free' dehydroascorbic acid was present in all 10 plants in small quantity. It fluctuated from time to time but remained fairly constant for all the plants during the period of investigation, and was independent of the 'total' value for that plant. The values for ascorbic acid have been measured for ripe strawberries, for fiddleheads, and for several common vegetables over the same summer months. They have been tabulated for comparison with the values of ascorbic acid of the 10 wild plants.

VARIATION IN GLUTATHIONE AND ASCORBIC ACID CONTENT AMONG SELECTED CULTIVARS OF Phaseolus vulgaris PRIOR TO AND AFTER EXPOSURE TO OZONE

1983 ◽  
Vol 63 (3) ◽  
pp. 733-737 ◽  
Author(s):  
ASAF GURI
Keyword(s):  
Ascorbic Acid ◽  
Phaseolus Vulgaris ◽  
Glutathione Reductase ◽  
Acid Content ◽  
Specific Activity ◽  
Reduced Glutathione ◽  
Ascorbic Acid Content ◽  
Ozone Exposure ◽  
Reduced Form ◽  
Oxidized Glutathione

Measurements of reduced glutathione (GSH) and ascorbic acid (AA) concentrations in the primary leaves of four bean cultivars, two ozone-sensitive (S) and two ozone-insensitive (I), have revealed that GSH concentrations were not significantly different in all four cultivars prior to the exposure to ozone (0.28–0.32 ppm for 8 h); however, after ozone exposure GSH concentrations were significantly lower in the two sensitive cultivars, PHR and 0669, while in the two insensitive tolerants, FH and Nep-2, the drop in GSH concentrations was slight. The assay of glutathione reductase (GR), the enzyme which catalyzed the reduction of oxidized glutathione (GSSG) to its reduced form, namely GSH, indicated that its specific activity in the two insensitive cultivars was almost twice as high as in the two sensitive cultivars. The drop in AA concentration after 8 h of fumigation was moderate (although significant) in all four cultivars, whereas the concentrations of AA in all four cultivars prior to ozone fumigation were not significantly different. From the data it is suggested that differences in GR-specific activity, probably due to different isozymes, could be the reason for insensitivity or sensitivity to ozone among bean cultivars.Key words: Ozone, Phaseolus, glutathione, ascorbic acid, glutathione reductase

ASCORBIC ACID IN RED RASPBERRY FRUIT AND LEAVES

1970 ◽  
Vol 50 (4) ◽  
pp. 457-461 ◽  
Author(s):  
S. O. FEJER ◽  
F. B. JOHNSTON ◽  
MADELEINE M. HAMMILL ◽  
L. P. S. SPANGELO
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Dehydroascorbic Acid ◽  
Ascorbic Acid Content ◽  
Leaf Fall ◽  
Red Raspberry ◽  
Present Stage ◽  
Present Material ◽  
Positive Correlations ◽  
Selection Of

Ascorbic acid content of leaves of 30 red raspberry clones was much higher than that of the fruits. In spite of seasonal variation both in leaf and fruit, ascorbic acid content of any clone was relatively similar in the same seasons of two consecutive years. However, the relation of leaf to fruit ascorbic acid was very variable between harvests, ranging from significantly negative to positive correlations. Thus, a selection of high ascorbic acid in the leaf to increase this compound in the fruit is not possible at the present stage, although there were indications that the content of the leaf is inherited by the seedlings. Dehydroascorbic acid was very low in the fall-fruiting Comet raspberry. High ascorbic acid content was related to early leaf fall in the present material.

Concentrations of vitamin C in milk of sows and in plasma of piglets

1995 ◽  
Vol 75 (2) ◽  
pp. 275-277 ◽  
Author(s):  
M. Hidiroglou ◽  
T. R. Batra
Keyword(s):  
Ascorbic Acid ◽  
Blood Plasma ◽  
Acid Concentration ◽  
Acid Content ◽  
High Performance ◽  
Dehydroascorbic Acid ◽  
Ascorbic Acid Content ◽  
Plasma Ascorbic Acid ◽  
Maternal Milk ◽  
Key Words Ascorbic Acid

Colostrum or milk samples from 10 sows and blood plasma samples from 68 piglets were taken between 0 and 28 d after farrowing and analyzed for ascorbic acid and dehydroascorbic acid by high-performance liquid chromatography with electro-chemical detector. Ascorbic acid content of colostrum (265.0 μm mL−1) was more than twice that of subsequently produced milk (100.0 μg mL−1) at 7 d of age. Ascorbic acid content of blood plasma of piglets at birth after uptake of colostrum was 13.1 μg mL−1, which slowly declined during the first 28 d of age to 3.2 μg mL−1. The dehydroascorbic acid concentration in the plasma of piglets ranged from 3.0 μg mL−1 to 3.9 μg mL−1from 7 d to 28 d of age. There was a significant positive correlation between piglet plasma ascorbic acid and maternal milk ascorbic acid concentration. Key words: Ascorbic acid, dehydroascorbic acid, colostrum

Analysis of ascorbic acid content in various fruits and vegetables by spectrofluorimetric methods

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
A Haskovic ◽  
A Copra Janicijevic ◽  
A Topcagic ◽  
L Klepo ◽  
A Kapur ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Fruits And Vegetables ◽  
Ascorbic Acid Content

scholarly journals Silicon attenuates calcium deficiency by increasing ascorbic acid content, growth and quality of cabbage leaves

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dalila Lopes da Silva ◽  
Renato de Mello Prado ◽  
Luis Felipe Lata Tenesaca ◽  
José Lucas Farias da Silva ◽  
Ben-Hur Mattiuz
Keyword(s):  
Ascorbic Acid ◽  
Nutrient Solution ◽  
Acid Content ◽  
Dry Matter ◽  
Ascorbic Acid Content ◽  
Calcium Deficiency ◽  
Ca Deficiency ◽  
Leaf Water Loss ◽  
Nutrient Solutions

AbstractCalcium (Ca) deficiency in cabbage plants induces oxidative damage, hampering growth and decreasing quality, however, it is hypothesized that silicon (Si) added to the nutrient solution may alleviate crop losses. Therefore, this study aims at evaluating whether silicon supplied in the nutrient solution reduces, in fact, the calcium deficiency effects on cabbage plants. In a greenhouse, cabbage plants were grown using nutrient solutions with Ca sufficiency and Ca deficiency (5 mM) without and with added silicon (2.5 mM), arranged as a 2 × 2 factorial in randomized blocks, with five replications. At 91 days after transplanting, the plants were harvested for biological evaluations. In the treatment without added Si, Ca deficiency promoted oxidative stress, low antioxidant content, decreased dry matter, and lower quality leaf. On the other hand, added Si attenuated Ca deficiency in cabbage by decreasing cell extravasation while increasing both ascorbic acid content and fresh and dry matter, providing firmer leaves due to diminished leaf water loss after harvesting. We highlighted the agronomic importance of Si added to the nutrient solution, especially in crops at risk of Ca deficiency.

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