scholarly journals Determination of interaction sites of phospholipase D1 for RhoA

2001 ◽  
Vol 355 (3) ◽  
pp. 779-785 ◽  
Author(s):  
Songmin CAI ◽  
John H. EXTON
Keyword(s):  
Dependent Manner ◽  
Interaction Site ◽  
Interaction Sites ◽  
Kinase C ◽  
C Terminus ◽  
Phospholipase D1 ◽  
A Site ◽  
Adp Ribosylation Factor ◽  
Made In

Phospholipase D (PLD) is regulated by many factors, including protein kinase C (PKC) and small G-proteins of the Rho and ADP-ribosylation factor families. Previous studies revealed that the interaction site of human PLD1 for RhoA is located in its C-terminus, but the exact locus has not been determined. The purpose of the present study was to determine the interaction site of rat PLD1 (rPLD1) with RhoA. Selection with phage display of different peptides of rPLD1 confirmed that GTP-bound RhoA interacted with a site in the amino acid sequence 873–1024 at the C-terminus of rPLD1. RhoA also associated with this peptide in a GTP-dependent manner in COS-7 cell lysates and the peptide inhibited RhoA stimulation of PLD activity in membranes from COS-7 cells expressing rPLD1. A series of alanine mutations of non-conserved residues were made in this sequence, and the enzymes were expressed in COS-7 cells and checked for responses to activation of PKC, which interacts with the N-terminus of PLD1, and also to the constitutively active V14RhoA. Mutations in the C-terminus of rPLD1 (K946A, V950A, R955A and K962A) caused partial loss of V14RhoA stimulation, and double mutations (K946A/K962A, K946A/V950A and K962A/V950A) caused an almost total loss. Co-immunoprecipitation studies also showed that the mutated forms of rPLD1 described above failed to bind V14RhoA compared with wild-type rPLD1, whereas rPLD1 with mutations outside the region K946–K962 bound V14RhoA normally. It is concluded that basic amino acids in a restricted C-terminal region of rPLD1 are important for binding of RhoA and its activation of PLD activity.

scholarly journals C-terminal peptides of rhodopsin. Determination of the optimum sequence for recognition of retinal transducin

1986 ◽  
Vol 235 (1) ◽  
pp. 309-312 ◽  
Author(s):  
D J Takemoto ◽  
D Morrison ◽  
L C Davis ◽  
L J Takemoto
Keyword(s):  
Amino Acid Residues ◽  
Guanine Nucleotide Binding Protein ◽  
Bovine Rhodopsin ◽  
C Terminus ◽  
Retinal Rod ◽  
Gamma Subunits ◽  
Guanine Nucleotide Binding ◽  
A Site ◽  
Optimum Sequence

In vertebrate retinal rod outer segments, transducin, a guanine-nucleotide-binding protein, mediates signal coupling between rhodopsin and cyclic GMP phosphodiesterase. Whereas the T alpha subunit (39 kDa) of transducin binds guanine nucleotides and is the activator of the phosphodiesterase, the T beta gamma subunits (35 and 10 kDa) may function to physically link T alpha with photolysed rhodopsin. We have previously reported that a site of binding of transducin is on the C-terminus of bovine rhodopsin. By using competition with synthetic peptides, the recognition region was localized to bovine opsin amino acid residues 317-339. Further studies are detailed which determine the boundaries of this binding site on rhodopsin, as well as some of the critical amino acids needed for transducin binding. These results suggest that the serine and threonine residues in the rhodopsin C-terminal peptides Rhod-1 and Rhod-3 are critical for reconstitution of transducin GTPase activity.

scholarly journals Structural basis for the binding of tryptophan-based motifs by δ-COP

2015 ◽  
Vol 112 (46) ◽  
pp. 14242-14247 ◽  
Author(s):  
Richard J. Suckling ◽  
Pak Phi Poon ◽  
Sophie M. Travis ◽  
Irina V. Majoul ◽  
Frederick M. Hughson ◽  
...  
Keyword(s):  
Adaptor Protein ◽  
Structural Basis ◽  
Titration Calorimetry ◽  
Carboxypeptidase Y ◽  
Binding Partner ◽  
C Terminus ◽  
Tryptophan Residues ◽  
A Site ◽  
Adp Ribosylation Factor ◽  
Unstructured Regions

Coatomer consists of two subcomplexes: the membrane-targeting, ADP ribosylation factor 1 (Arf1):GTP-binding βγδζ-COP F-subcomplex, which is related to the adaptor protein (AP) clathrin adaptors, and the cargo-binding αβ’ε-COP B-subcomplex. We present the structure of the C-terminal μ-homology domain of the yeast δ-COP subunit in complex with the WxW motif from its binding partner, the endoplasmic reticulum-localized Dsl1 tether. The motif binds at a site distinct from that used by the homologous AP μ subunits to bind YxxΦ cargo motifs with its two tryptophan residues sitting in compatible pockets. We also show that the Saccharomyces cerevisiae Arf GTPase-activating protein (GAP) homolog Gcs1p uses a related WxxF motif at its extreme C terminus to bind to δ-COP at the same site in the same way. Mutations designed on the basis of the structure in conjunction with isothermal titration calorimetry confirm the mode of binding and show that mammalian δ-COP binds related tryptophan-based motifs such as that from ArfGAP1 in a similar manner. We conclude that δ-COP subunits bind Wxn(1–6)[WF] motifs within unstructured regions of proteins that influence the lifecycle of COPI-coated vesicles; this conclusion is supported by the observation that, in the context of a sensitizing domain deletion in Dsl1p, mutating the tryptophan-based motif-binding site in yeast causes defects in both growth and carboxypeptidase Y trafficking/processing.

Phosphorylation and Activation of Phospholipase D1 by Protein Kinase C in Vivo:  Determination of Multiple Phosphorylation Sites†

Biochemistry ◽  
1999 ◽  
Vol 38 (32) ◽  
pp. 10344-10351 ◽  
Author(s):  
Yong Kim ◽  
Jung Min Han ◽  
Jong Bae Park ◽  
Sang Do Lee ◽  
Yong Seok Oh ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Phosphorylation Sites ◽  
Kinase C ◽  
Phospholipase D1

The quantitative comparison of experimental and simulated diffraction contrast images

1995 ◽  
Vol 53 ◽  
pp. 102-103
Author(s):  
Stuart McKernan
Keyword(s):  
Image Processing ◽  
Personal Computer ◽  
Close Agreement ◽  
Quantitative Comparison ◽  
Image Simulation ◽  
Computing Power ◽  
Diffraction Contrast ◽  
Simulated Images ◽  
Made In

For many years the concept of quantitative diffraction contrast experiments might have consisted of the determination of dislocation Burgers vectors using a g.b = 0 criterion from several different 2-beam images. Since the advent of the personal computer revolution, the available computing power for performing image-processing and image-simulation calculations is enormous and ubiquitous. Several programs now exist to perform simulations of diffraction contrast images using various approximations. The most common approximations are the use of only 2-beams or a single systematic row to calculate the image contrast, or calculating the image using a column approximation. The increasing amount of literature showing comparisons of experimental and simulated images shows that it is possible to obtain very close agreement between the two images; although the choice of parameters used, and the assumptions made, in performing the calculation must be properly dealt with. The simulation of the images of defects in materials has, in many cases, therefore become a tractable problem.

Endotoxin-Induced Tissue Factor in Human Monocytes is Dependent upon Protein Kinase C Activation

1993 ◽  
Vol 70 (05) ◽  
pp. 800-806 ◽  
Author(s):  
C Ternisien ◽  
M Ramani ◽  
V Ollivier ◽  
F Khechai ◽  
T Vu ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Tissue Factor ◽  
Factor Ix ◽  
Transcriptional Level ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Human Monocytes ◽  
Kinase C ◽  
Pkc Activation

SummaryTissue factor (TF) is a transmembrane receptor which, in association with factors VII and Vila, activates factor IX and X, thereby activating the coagulation protease cascades. In response to bacterial lipopolysaccharide (LPS) monocytes transcribe, synthesize and express TF on their surface. We investigated whether LPS-induced TF in human monocytes is mediated by protein kinase C (PKC) activation. The PKC agonists phorbol 12- myristate 13-acetate (PMA) and phorbol 12, 13 dibutyrate (PdBu) were both potent inducers of TF in human monocytes, whereas 4 alpha-12, 13 didecanoate (4 a-Pdd) had no such effect. Both LPS- and PMA-induced TF activity were inhibited, in a concentration dependent manner, by three different PKC inhibitors: H7, staurosporine and calphostin C. TF antigen determination confirmed that LPS-induced cell-surface TF protein levels decreased in parallel to TF functional activity under staurosporine treatment. Moreover, Northern blot analysis of total RNA from LPS- or PMA-stimulated monocytes showed a concentration-dependent decrease in TF mRNA levels in response to H7 and staurosporine. The decay rate of LPS-induced TF mRNA evaluated after the arrest of transcription by actinomycin D was not affected by the addition of staurosporine, suggesting that its inhibitory effect occurred at a transcriptional level. We conclude that LPS-induced production of TF and its mRNA by human monocytes are dependent on PKC activation.

Science of Ahangyol and Its Importance for Modern Scientific Thought

2019 ◽  
Vol 10 (5) ◽  
pp. 473-478
Author(s):  
Ahmad Gashamoglu ◽  
Keyword(s):  
Modern Science ◽  
Scientific Basis ◽  
New Paradigm ◽  
Islamic Philosophy ◽  
Scientific Thought ◽  
Religious Books ◽  
Strategic Approach ◽  
Scientific Potential ◽  
Made In

The Article briefly discusses the need for generation of the Science of Ahangyol, and this science’s scientific basis, object and subject, category system, scientific research methods and application options. Ahangyol is a universal science and may be useful in any sphere. It may assist in problem solving in peacemaking process and in many areas such as ecology, economics, politics, culture, management and etc. This science stipulates that any activity and any decision made in the life may only and solely be successful when they comply with harmony principles more, which are the principles of existence and activity of the world. A right strategic approach of the Eastern Philosophy and the Middle Age Islamic Philosophy and scientific thought has an important potential. This strategic approach creates opportunities to also consider irrational factors in addition to rational ones comprehensively in scientific researches. The modern scientific thought contributes to implementation of these opportunities. Ahangyol is a science of determination of ways to achieve harmony in any sphere and of creation of special methods to make progress in these ways through assistance of the modern science. Methods of the System Theory, Mathematics, IT, Astronomy, Physics, Biology, Sociology, Statistics and etc. are more extensively applied. Information is given on some of these methods. Moreover, the Science of Ahangyol, which is a new philosophical worldview and a new paradigm contributes to clarification of metaphysic views considerably and discovery of the scientific potential of religious books.

Wing lengths of Clamorous Reed Warblers Acrocephalus stentoreus in Israel

Ring ◽  
2004 ◽  
Vol 26 (2) ◽  
pp. 89-92
Author(s):  
John Morgan
Keyword(s):  
Wing Length ◽  
First Year ◽  
Comparable Data ◽  
Reed Warbler ◽  
Data Set ◽  
Acrocephalus Scirpaceus ◽  
Different Seasons ◽  
A Site ◽  
Made In ◽  
New Discovery

Wing lengths of Clamorous Reed Warblers Acrocephalus stentoreus in Israel Wing length measurements taken from first-year, pre- and post-moulting (annual, complete) Clamorous Reed Warblers were recorded at a site in northern Israel. The resulting data set was examined using a time-series of residuals (CUSUM). Results from this analysis can explain the reported heterogeneity found in a comparable data set by Merom et al. (1999). Further observations made in their paper are rebutted: (1) an implied assumption that Reed Warbler (Acrocephalus scirpaceus) spring migration in Israel ends by 1 May is contrary to other publications; (2) the late autumn occurence in N Israel of longer-winged 1st cal. yr. Reed Warblers, unconvincingly explained as either delayed migration by larger individuals or post fledging feather growth, is most likely due to birds from different provenances origins moving at different seasons; (3) growth during adulthood in Reed Warbler is not a new discovery, though presented as such.

scholarly journals Determination of Environmental Factors for the Implementation of the Exploitability Index in Industrial Aggregate Mining Using Multi-Criteria Analysis

2021 ◽  
Vol 10 (4) ◽  
pp. 196
Author(s):  
Julio Manuel de Luis-Ruiz ◽  
Benito Ramiro Salas-Menocal ◽  
Gema Fernández-Maroto ◽  
Rubén Pérez-Álvarez ◽  
Raúl Pereda-García
Keyword(s):  
Environmental Factors ◽  
Environmental Factor ◽  
Human Life ◽  
Accurate Estimation ◽  
Analytic Hierarchy ◽  
Main Components ◽  
Hierarchy Process ◽  
Made In

The quality of human life is linked to the exploitation of mining resources. The Exploitability Index (EI) assesses the actual possibilities to enable a mine according to several factors. The environment is one of the most constraining ones, but its analysis is made in a shallow way. This research is focused on its determination, according to a new preliminary methodology that sets the main components of the environmental impact related to the development of an exploitation of industrial minerals and its weighting according to the Analytic Hierarchy Process (AHP). It is applied to the case of the ophitic outcrops in Cantabria (Spain). Twelve components are proposed and weighted with the AHP and an algorithm that allows for assigning a normalized value for the environmental factor to each deposit. Geographic Information Systems (GISs) are applied, allowing us to map a large number of components of the environmental factors. This provides a much more accurate estimation of the environmental factor, with respect to reality, and improves the traditional methodology in a substantial way. It can be established as a methodology for mining spaces planning, but it is suitable for other contexts, and it raises developing the environmental analysis before selecting the outcrop to be exploited.

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