scholarly journals The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium Bacillus subtilis

2000 ◽  
Vol 350 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Keith STEPHENSON ◽  
Christina L. JENSEN ◽  
Steen T. JØRGENSEN ◽  
Jeremy H. LAKEY ◽  
Colin R. HARWOOD
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Culture Medium ◽  
Sequence Data ◽  
Chemical Properties ◽  
Secretory Protein ◽  
Secretory Proteins ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus ◽  
Late Stages

Following their secretion across the cytoplasmic membrane, processed secretory proteins of Bacillus subtilis must fold into their native conformation prior to translocation through the cell wall and release into the culture medium. The rate and efficiency of folding are critical in determining the yields of intact secretory proteins. The B. subtilis membrane is surrounded by a thick cell wall comprising a heteropolymeric matrix of peptidoglycan and anionic polymers. The latter confer a high density of negative charge on the wall, endowing it with ion-exchange properties, and secretory proteins destined for the culture medium must traverse the wall as the last stage in the export process. To determine the influence of charge on late stages in the secretion of proteins from this bacterium, we have used sequence data from two related α-amylases, to engineer the net charge of AmyL, an α-amylase from Bacillus licheniformis that is normally secreted efficiently from B. subtilis. While AmyL has a pI of 7.0, chimaeric enzymes with pI values of 5.0 and 10.0 were produced and characterized. Despite the engineered changes to their physico-chemical properties, the chimaeric enzymes retained many of the enzymic characteristics of AmyL. We show that the positively charged protein interacts with the cell wall in a manner that influences its secretion.

scholarly journals The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium Bacillus subtilis

2000 ◽  
Vol 351 (3) ◽  
pp. 839-839
Author(s):  
K. STEPHENSON ◽  
C. L. JENSEN ◽  
S. T. JØRGENSEN ◽  
J. H. LAKEY ◽  
C. R. HARWOOD
Keyword(s):  
Bacillus Subtilis ◽  
Secretory Protein ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Protein Charge ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus ◽  
Late Stages

scholarly journals The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium Bacillus subtilis

2000 ◽  
Vol 350 (1) ◽  
pp. 31 ◽  
Author(s):  
Keith STEPHENSON ◽  
Christina L. JENSEN ◽  
Steen T. JØRGENSEN ◽  
Jeremy H. LAKEY ◽  
Colin R. HARWOOD
Keyword(s):  
Bacillus Subtilis ◽  
Secretory Protein ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Protein Charge ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus ◽  
Late Stages

scholarly journals Use of starch manufacturer residue in the production of inoculating biofertilizer

2019 ◽  
Vol 23 ◽  
pp. 13
Author(s):  
Paulo Agenor Alves Bueno ◽  
Edgar Lopes Balestri ◽  
Rafael De Almeida Vidal Feres Rosiello ◽  
Cristian Coelho Silva ◽  
Bruna Lopes Gualdi ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Culture Medium ◽  
Low Cost ◽  
Growth Promoters ◽  
Trichoderma Spp ◽  
Environmental Liabilities ◽  
Maximum Production ◽  
Plant Growth Promoters ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

In the industrialization of cassava, effluents are generated capable of causing great impacts to the environment. One of these residues is called manipueira and its generation occurs by pressing the grated cassava mass. An alternative to the use of this industrial residue is its use as a culture medium in the multiplication of microorganisms useful in agriculture in the form of biological inoculants. The objective of this work was to evaluate the effectiveness of different concentrations of manipueira used as substrate in the development of the bacterium Bacillus subtilis and the fungus Trichoderma spp as biological control agents and plant growth promoters, aiming at the low cost of the agricultural protection process. The tests were carried out by means of a triple plating technique in culture medium containing manipueira, molasses and distilled water in different concentrations for both biological agents. The efficiency of the method, substract and organisms was measured through the calculation of colony forming units (CFU). The maximum production of CFU of the fungus Trichoderma spp was 1.2x109 CFU.mL -1 in a concentration of 175mL.L -1 of manipueira, while for the bacterium Bacillus subtilis, the maximum production reached 6.49x1011 CFU .mL -1 in culture with concentration of 125mL -1 of the residue. Compared to commercial inoculants with concentration around 1x108 CFU, the tested cultures were compatible with this value and still managed to overcome it, confirming its efficiency. Therefore, this is an excellent possibility to direct the use of environmental liabilities and to encourage the use of the biofertilizer by farmers who can produce it in their properties in an alternative way and with a low cost of production in relation to the commercial value.

scholarly journals Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Naomi Shimokawa-Chiba ◽  
Claudia Müller ◽  
Keigo Fujiwara ◽  
Bertrand Beckert ◽  
Koreaki Ito ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stop Codon ◽  
Release Factor ◽  
Positive Bacterium ◽  
Gram Positive ◽  
E Coli ◽  
Cryo Electron Microscopy ◽  
Dead End ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

AbstractRescue of the ribosomes from dead-end translation complexes, such as those on truncated (non-stop) mRNA, is essential for the cell. Whereas bacteria use trans-translation for ribosome rescue, some Gram-negative species possess alternative and release factor (RF)-dependent rescue factors, which enable an RF to catalyze stop-codon-independent polypeptide release. We now discover that the Gram-positive Bacillus subtilis has an evolutionarily distinct ribosome rescue factor named BrfA. Genetic analysis shows that B. subtilis requires the function of either trans-translation or BrfA for growth, even in the absence of proteotoxic stresses. Biochemical and cryo-electron microscopy (cryo-EM) characterization demonstrates that BrfA binds to non-stop stalled ribosomes, recruits homologous RF2, but not RF1, and induces its transition into an open active conformation. Although BrfA is distinct from E. coli ArfA, they use convergent strategies in terms of mode of action and expression regulation, indicating that many bacteria may have evolved as yet unidentified ribosome rescue systems.

Glycosylated Methoxy-Macrolactins from a Marine Sediment Bacterium Bacillus subtilis

Heterocycles ◽  
2013 ◽  
Vol 87 (2) ◽  
pp. 307 ◽  
Author(s):  
Hee Jae Shin ◽  
Fakir Shahidullah Tareq ◽  
Ji Hye Kim ◽  
Min Ah Lee ◽  
Hyi-Seung Lee ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Marine Sediment ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

Uncoupling and toxic action of alkyltriphenylphosphonium cations on mitochondria and the bacterium Bacillus subtilis as a function of alkyl chain length

2015 ◽  
Vol 80 (12) ◽  
pp. 1589-1597 ◽  
Author(s):  
L. S. Khailova ◽  
P. A. Nazarov ◽  
N. V. Sumbatyan ◽  
G. A. Korshunova ◽  
T. I. Rokitskaya ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Chain Length ◽  
Alkyl Chain ◽  
Alkyl Chain Length ◽  
Toxic Action ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

Fractionation of an Aquatic Fulvic Acid upon Adsorption to the Bacterium,Bacillus subtilis

2004 ◽  
Vol 21 (2) ◽  
pp. 69-78 ◽  
Author(s):  
Patricia A. Maurice ◽  
Maciej Manecki ◽  
Jeremy B. Fein ◽  
Jennifer Schaefer
Keyword(s):  
Bacillus Subtilis ◽  
Fulvic Acid ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus
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