scholarly journals Expression of the rat liver carnitine palmitoyltransferase I (CPT-Iα) gene is regulated by Sp1 and nuclear factor Y: chromosomal localization and promoter characterization

1999 ◽  
Vol 340 (2) ◽  
pp. 425-432 ◽  
Author(s):  
Michelle L. STEFFEN ◽  
Wilbur R. HARRISON ◽  
Frederick F. B. ELDER ◽  
George A. COOK ◽  
Edwards A. PARK
Keyword(s):  
Gene Expression ◽  
Carnitine Palmitoyltransferase ◽  
Chromosome 1 ◽  
Proximal Promoter ◽  
Nuclear Factor ◽  
Membrane Expression ◽  
Base Pairs ◽  
Nuclear Factor Y ◽  
Specific Regulation ◽  
Cpt I

Carnitine palmitoyltransferase (CPT)-I catalyses the transfer of long-chain fatty acids from CoA to carnitine for translocation across the mitochondrial inner membrane. Expression of the ‘liver’ isoform of the CPT-I gene (CPT-Iα) is subject to developmental, hormonal and tissue-specific regulation. To understand the basis for control of CPT-Iα gene expression, we have characterized the proximal promoter of the CPT-Iα gene. Here, we report the sequence of 6839 base pairs of the promoter and the localization of the rat CPT-Iα gene to region q43 on chromosome 1. Our studies show that the first 200 base pairs of the promoter are sufficient to drive transcription of the CPT-Iα gene. Within this region are two sites that bind both Sp1 and Sp3 transcription factors. In addition, nuclear factor Y (NF-Y) binds the proximal promoter. Mutation at the Sp1 or NF-Y sites severely decreases transcription from the CPT-Iα promoter. Other protein binding sites were identified within the first 200 base pairs of the promoter by DNase I footprinting, and these elements contribute to CPT-Iα gene expression. Our studies demonstrate that CPT-Iα is a TATA-less gene which utilizes NF-Y and Sp proteins to drive basal expression.

scholarly journals Cloning and characterization of the promoter for the liver isoform of the rat carnitine palmitoyltransferase I (L-CPT I) gene

1998 ◽  
Vol 330 (1) ◽  
pp. 217-224 ◽  
Author(s):  
A. Edwards PARK ◽  
L. Michelle STEFFEN ◽  
Shulan SONG ◽  
M. Vicki PARK ◽  
A. George COOK
Keyword(s):  
Genomic Library ◽  
Transcriptional Start Site ◽  
Carnitine Palmitoyltransferase ◽  
Proximal Promoter ◽  
Exon 2 ◽  
Exon 1 ◽  
Carnitine Palmitoyltransferase I ◽  
Specific Regulation ◽  
Cpt I ◽  
I Gene

Carnitine palmitoyltransferase I (CPT I) catalyses the transfer of long chain fatty acids to carnitine for translocation across the mitochondrial inner membrane. The cDNAs of two isoforms of CPT I, termed the hepatic and muscle isoforms, have been cloned. Expression of the hepatic CPT I gene (L-CPT I) is subject to developmental, hormonal and tissue specific regulation. We have cloned the promoter of the L-CPT I gene from a rat genomic library. In the L-CPT I gene, there are two exons 5ʹ to the exon containing the ATG that initiates translation. Exon 1 and the 5ʹ end of exon 2 contain sequences that were not previously described in the rat L-CPT I cDNA. There is an alternatively spliced form of the L-CPT I mRNA in which exon 2 is skipped. The proximal promoter of the L-CPT I gene is extremely GC rich and does not contain a TATA box. There are several putative Sp1 binding sites near the transcriptional start site. A 190 base pair fragment of the promoter can efficiently drive transcription of luciferase and CAT (chloramphenicol acetyltransferase) reporter genes transiently transfected into HepG2 cells. Sequences in both the first intron and the promoter contribute to basal expression. Our results provide the foundation for further studies into the regulation of L-CPTI gene expression.

scholarly journals CCAAT/Enhancer Binding Protein and Nuclear Factor-Y Regulate Adiponectin Gene Expression in Adipose Tissue

Diabetes ◽  
2004 ◽  
Vol 53 (11) ◽  
pp. 2757-2766 ◽  
Author(s):  
S.-k. Park ◽  
S.-Y. Oh ◽  
M.-Y. Lee ◽  
S. Yoon ◽  
K.-S. Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Binding Protein ◽  
Nuclear Factor ◽  
Adiponectin Gene ◽  
Nuclear Factor Y ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein

scholarly journals Expression of the Hepatic Specific V1 Messenger Ribonucleic Acid of the Human Growth Hormone Receptor Gene Is Regulated by Hepatic Nuclear Factor (HNF)-4α2 and HNF-4α8

2008 ◽  
Vol 22 (2) ◽  
pp. 485-500 ◽  
Author(s):  
Cynthia Cynthia ◽  
Zakaria Rhani ◽  
Hong Zheng
Keyword(s):  
Growth Hormone Receptor ◽  
Receptor Gene ◽  
Regulatory Region ◽  
Human Growth ◽  
Regulatory Elements ◽  
Proximal Promoter ◽  
Target Cells ◽  
Nuclear Factor ◽  
Messenger Ribonucleic Acid ◽  
Specific Regulation

Abstract Human (h) GH plays an essential role in growth and metabolism, and its effectiveness is modulated by the availability of its specific receptor [hGH receptor (hGHR)] on target cells. The hGHR gene has a complex 5′-regulatory region containing multiple first exons. Seven are clustered within two small regions: V2,V3,V9 (module A) and V1,V4,V7,V8 (module B). Module A-derived mRNAs are ubiquitously expressed whereas those from module B are only found in postnatal liver, suggesting developmental- and liver-specific regulation of module B hGHR gene expression. To characterize the elements regulating module B activity, we studied a 1.8-kb promoter of the highest expressing exon in liver, V1. This promoter was repressed in transfection assays; however, either 5′- or 3′-deletions relieved this, suggesting the presence of multiple negative regulatory elements. Six putative hepatic nuclear factor 4 (HNF-4) response elements were identified. We determined that HNF-4α is developmentally regulated in the human liver: HNF-4α2 and HNF-4α8 are expressed in fetal hepatocytes but only HNF-4α2 is expressed in postnatal liver. Transient transfection assays demonstrated that HNF-4α2 and HNF-4α8 have a similar dual effect on V1 transcription: activation via site 1 in the proximal promoter and repression through site 6, approximately 1.7 kb upstream. EMSA/electrophoretic mobility supershift assays and chromatin immunoprecipitation analyses confirmed these two sites are bound by HNF-4α. Based on these data, we speculate there are multiple regions working together to repress the expression of V1 hGHR transcripts in tissues other than the normal postnatal liver, and that HNF-4α is a good candidate for regulating V1 hGHR expression in the human hepatocyte.

scholarly journals Cloning and Characterization of a 5′ Regulatory Region of the Prolactin Receptor-Associated Protein/17β Hydroxysteroid Dehydrogenase 7 Gene

Endocrinology ◽  
2005 ◽  
Vol 146 (6) ◽  
pp. 2807-2816 ◽  
Author(s):  
Michael Risk ◽  
Aurora Shehu ◽  
Jifang Mao ◽  
Carlos O. Stocco ◽  
Laura T. Goldsmith ◽  
...  
Keyword(s):  
Cell Line ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Prolactin Receptor ◽  
Hydroxysteroid Dehydrogenase ◽  
Proximal Promoter ◽  
Luteal Cell ◽  
Nuclear Factor ◽  
Receptor Associated Protein ◽  
Nuclear Factor Y

Abstract Prolactin receptor-associated protein (PRAP) originally cloned in our laboratory was shown to be a novel, luteal isoform of 17β hydroxysteroid dehydrogenase 7 (17βHSD7). In this study, we cloned the promoter region of rat PRAP/17βHSD7 and investigated the mechanisms regulating both basal activity and LH-induced repression of this promoter. Truncated and site-specific mutants of PRAP/17βHSD7 promoter identified two enhancer regions that contained highly conserved Sp1 binding site and bound Sp1 from nuclear extracts of both corpora lutea and a rat luteal cell line. Repression of PRAP/17βHSD7 expression and promoter activity by human chorionic gonadotropin/forskolin was localized to a −52-bp proximal segment of the promoter. This region contained a conserved CCAAT site and bound nuclear factor Y; binding of this transcription factor was inhibited by human chorionic gonadotropin in vivo. Furthermore, mutation of the nuclear factor Y site in the −52-bp promoter-reporter construct abolished forskolin-mediated inhibition of the promoter in a rat luteal cell line. In summary, we have identified the promoter elements involved in the basal expression of PRAP/17βHSD7. We have also found that LH-mediated repression of this gene is at the level of transcription and involves inhibition of nuclear factor YA binding to the CCAAT site within the proximal promoter.

scholarly journals Developmental changes in carnitine palmitoyltransferases I and II gene expression in intestine and liver of suckling rats

1995 ◽  
Vol 306 (2) ◽  
pp. 379-384 ◽  
Author(s):  
G Asins ◽  
D Serra ◽  
G Arias ◽  
F G Hegardt
Keyword(s):  
Gene Expression ◽  
Similar Pattern ◽  
Carnitine Palmitoyltransferase ◽  
Developmental Changes ◽  
Mrna Levels ◽  
Appreciable Change ◽  
Suckling Rats ◽  
Mother's Milk ◽  
Cpt I ◽  
Carnitine Palmitoyltransferases

Carnitine palmitoyltransferase (CPT) I is expressed in the intestine of suckling rats; its mRNA increases very rapidly after birth, remains on a plateau until day 18 and decreases until weaning, when basal (adult) values are reached, which remain unchanged thereafter. CPT II mRNA values do not show any appreciable change in this period. CPT I and CPT II are expressed mainly in mucosa and, to a lesser extent, in the muscular part of the intestine. Intestinal expression of CPT I is maximal in duodenum and jejunum, whereas CPT II is expressed in a similar pattern throughout the whole intestine. Dam's milk may influence the intestinal expression of CPT I, since mRNA levels at birth are low but increase after the first lactation. Moreover, rats weaned at either day 18 or 21 decrease their mRNA levels. Apparently, CPT II gene expression is not influenced by the mother's milk. CPT I and CPT II are also expressed in the liver of suckling rats. Hepatic CPT I is maximal at day 3, and levels of CPT II mRNA do not change, in a similar fashion to that in intestine. The profile of expression of CPT I in liver and intestine strongly resembles that previously reported for mitochondrial 3-hydroxy-3-methyl-glutaryl-CoA synthase.

scholarly journals Nuclear Factor-Y is an adipogenic factor that regulates leptin gene expression

2015 ◽  
Vol 4 (5) ◽  
pp. 392-405 ◽  
Author(s):  
Yi-Hsueh Lu ◽  
Olof Stefan Dallner ◽  
Kivanc Birsoy ◽  
Gulya Fayzikhodjaeva ◽  
Jeffrey M. Friedman
Keyword(s):  
Gene Expression ◽  
Nuclear Factor ◽  
Nuclear Factor Y

Nuclear factor Y (NF-Y) regulates the proximal promoter activity of the mouse collagen α1(XI) gene (Col11a1) in chondrocytes

2013 ◽  
Vol 50 (4) ◽  
pp. 358-366 ◽  
Author(s):  
Mariko Hida ◽  
Ryoji Hamanaka ◽  
Osamu Okamoto ◽  
Kouhei Yamashita ◽  
Takako Sasaki ◽  
...  
Keyword(s):  
Promoter Activity ◽  
Proximal Promoter ◽  
Nuclear Factor ◽  
Nuclear Factor Y
Sign in / Sign up

Export Citation Format

Share Document