scholarly journals Identification of a Caenorhabditis elegans Δ6-fatty-acid-desaturase by heterologous expression in Saccharomyces cerevisiae

1998 ◽  
Vol 333 (3) ◽  
pp. 847-848
Author(s):  
J. A. NAPIER ◽  
S. J. HEY ◽  
D. J. LACEY ◽  
P. R. SHEWRY
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acid ◽  
Caenorhabditis Elegans ◽  
Heterologous Expression ◽  
Fatty Acid Desaturase

scholarly journals Identification of a Caenorhabditis elegans Δ6-fatty-acid-desaturase by heterologous expression in Saccharomyces cerevisiae

1998 ◽  
Vol 330 (2) ◽  
pp. 611-614 ◽  
Author(s):  
A. Johnathan NAPIER ◽  
J. Sandra HEY ◽  
J. Dominic LACEY ◽  
R. Peter SHEWRY
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acid ◽  
Caenorhabditis Elegans ◽  
Expressed Sequence Tag ◽  
Cytochrome B5 ◽  
Fatty Acid Desaturase ◽  
Higher Plant ◽  
Yeast Saccharomyces Cerevisiae ◽  
C Elegans ◽  
Δ6 Desaturase

We identified a cDNA expressed sequence tag from an animal (the nematode worm Caenorhabditis elegans) that showed weak similarity to a higher-plant microsomal Δ6-desaturase. A full-length cDNA clone was isolated and expressed in the yeast Saccharomyces cerevisiae. This demonstrated that the protein encoded by the C. elegans cDNA was that of a fatty acid Δ6-desaturase, as determined by the accumulation of γ-linolenic acid. The C. elegans Δ6-desaturase contained an N-terminalcytochrome b5 domain, indicating that it had a similar structure to that of the higher-plant Δ6-desaturase. The C. elegans Δ6-desaturase mapped to cosmid W08D2, a region of chromosome III. This is the first example of a Δ6-desaturase isolated from an animal and also the first example of an animal desaturase containing a cytochrome b5 domain.

scholarly journals Gene transfer of the Caenorhabditis elegans n-3 fatty acid desaturase inhibits neuronal apoptosis

2002 ◽  
Vol 82 (6) ◽  
pp. 1360-1366 ◽  
Author(s):  
Yinlin Ge ◽  
Xiaoying Wang ◽  
Zhihong Chen ◽  
Natalie Landman ◽  
Eng H. Lo ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Caenorhabditis Elegans ◽  
Gene Transfer ◽  
Neuronal Apoptosis ◽  
Fatty Acid Desaturase
Sign in / Sign up

Export Citation Format

Share Document