scholarly journals Genomic organization of the 3′-region of the human MUC5AC mucin gene: additional evidence for a common ancestral gene for the 11p15.5 mucin gene family

1998 ◽  
Vol 332 (3) ◽  
pp. 729-738 ◽  
Author(s):  
Marie-Pierre BUISINE ◽  
Jean-Luc DESSEYN ◽  
Nicole PORCHET ◽  
Pierre DEGAND ◽  
Anne LAINE ◽  
...  
Keyword(s):  
Gene Family ◽  
Genomic Organization ◽  
Splice Sites ◽  
Ancestral Gene ◽  
Remarkable Similarity ◽  
Mucin Gene ◽  
Isolation And Characterization ◽  
Entire Sequence ◽  
Chromosome 11P15.5

The human mucin gene MUC5AC is mapped clustered with MUC2, MUC5B and MUC6 on chromosome 11p15.5. We report here the isolation and characterization of a genomic cosmid clone, designated ELO9, spanning the 3´-region of MUC5AC and the 5´-region of MUC5B, allowing us to conclude that MUC5AC and MUC5B have the same transcriptional orientation. We determined the genomic organization and the entire sequence of the 3´-region of MUC5AC. The comparative molecular analysis of MUC5AC and MUC5B points to a remarkable similarity in the size and the distribution of exons, and in the type of splice sites, supporting the notion that MUC5AC and MUC5B have evolved from a single common ancestral gene. The derivation of the four genes of the 11p15.5 mucin gene family from a single ancestral gene is discussed.

scholarly journals Isolation and Characterization Of Rice R Genes: Evidence for Distinct Evolutionary Paths in Rice and Maize

Genetics ◽  
1996 ◽  
Vol 142 (3) ◽  
pp. 1021-1031 ◽  
Author(s):  
Jianping Hu ◽  
Beth Anderson ◽  
Susan R Wessler
Keyword(s):  
Gene Family ◽  
Gene Families ◽  
Chromosome 1 ◽  
R Gene ◽  
R Genes ◽  
Helix Loop Helix ◽  
Isolation And Characterization ◽  
Undetermined Function ◽  
Evolutionary Paths

Abstract R and B genes and their homologues encode basic helix-loop-helix (bHLH) transcriptional activators that regulate the anthocyanin biosynthetic pathway in flowering plants. In maize, R/B genes comprise a very small gene family whose organization reflects the unique evolutionary history and genome architecture of maize. To know whether the organization of the R gene family could provide information about the origins of the distantly related grass rice, we characterized members of the R gene family from rice Oryza sativa. Despite being a true diploid, O. sativa has at least two R genes. An active homologue (Ra) with extensive homology with other R genes is located at a position on chromosome 4 previously shown to be in synteny with regions of maize chromosomes 2 and 10 that contain the B and R loci, respectively. A second rice R gene (Rb) of undetermined function was identified on chromosome 1 and found to be present only in rice species with AA genomes. All non-AA species have but one R gene that is Ra-like. These data suggest that the common ancestor shared by maize and rice had a single R gene and that the small R gene families of grasses have arisen recently and independently.

scholarly journals Isolation and Characterization of Rat Cytochrome P-450IIB Gene Family Members

1989 ◽  
Vol 264 (12) ◽  
pp. 7046-7053 ◽  
Author(s):  
C M Giachelli ◽  
J Lin-Jones ◽  
C J Omiecinski
Keyword(s):  
Gene Family ◽  
Family Members ◽  
Isolation And Characterization

Isolation and characterization of theXenopusHIVEP gene family

2004 ◽  
Vol 271 (6) ◽  
pp. 1135-1144 ◽  
Author(s):  
Ulrike Dürr ◽  
Kristine A. Henningfeld ◽  
Thomas Hollemann ◽  
Walter Knöchel ◽  
Tomas Pieler
Keyword(s):  
Gene Family ◽  
Isolation And Characterization

Genomic Organization and Chromosomal Localization of a Member of the MAP Kinase Phosphatase Gene Family to Human Chromosome 11p15.5 and a Pseudogene to 10q11.2

Genomics ◽  
1997 ◽  
Vol 42 (2) ◽  
pp. 284-294 ◽  
Author(s):  
M.A. Nesbit ◽  
M.D. Hodges ◽  
L. Campbell ◽  
T.M.A.M.O. de Meulemeester ◽  
M. Alders ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Gene Family ◽  
Map Kinase ◽  
Genomic Organization ◽  
Chromosomal Localization ◽  
Map Kinase Phosphatase ◽  
Chromosome 11P15.5

Isolation and characterization of PKC-L, a new member of the protein kinase C-related gene family specifically expressed in lung, skin, and heart

1991 ◽  
Vol 11 (1) ◽  
pp. 126-133 ◽  
Author(s):  
N Bacher ◽  
Y Zisman ◽  
E Berent ◽  
E Livneh
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Gene Family ◽  
Phorbol Esters ◽  
Structural Features ◽  
Cos Cells ◽  
Isolation And Characterization ◽  
Kinase C ◽  
Human Epidermoid Carcinoma

We have isolated and characterized a new human cDNA, coding for a protein kinase, related to the protein kinase C (PKC) gene family. Although this protein kinase shares some homologous sequences and structural features with the four members of the PKC family initially isolated (alpha, beta I, beta II, and gamma), it shows more homology with the recently described PKC-related subfamily, encoded by the cDNAs delta, epsilon, and zeta. The transcript for this gene product, termed PKC-L, is most abundant in lung tissue, less expressed in heart and skin tissue, and exhibited very low expression in brain tissue. Thus, its tissue distribution is different from that described for other mammalian members of the PKC gene family, their expression being enriched in brain tissues. PKC-L is also expressed in several human cell lines, including the human epidermoid carcinoma line A431. The ability of phorbol esters to bind to and stimulate the kinase activity of PKC-L was revealed by introducing the cDNA into COS cells.

Rectification concerning “Isolation and characterization of a novel anthocyanin-promoting MYBA gene family in Citrus”

2012 ◽  
Vol 8 (4) ◽  
pp. 687-687
Author(s):  
Zhijian T. Li ◽  
Frederick G. Gmitter ◽  
Jude W. Grosser ◽  
Chunxian Chen ◽  
Dennis J. Gray
Keyword(s):  
Gene Family ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of the monkey UGT2B30 gene that encodes a uridine diphosphate-glucuronosyltransferase enzyme active on mineralocorticoid, glucocorticoid, androgen and oestrogen hormones

2002 ◽  
Vol 365 (1) ◽  
pp. 213-222 ◽  
Author(s):  
Caroline GIRARD ◽  
Olivier BARBIER ◽  
David TURGEON ◽  
Alain BÉLANGER
Keyword(s):  
Cynomolgus Monkey ◽  
Genomic Organization ◽  
Human Kidney ◽  
Relative Activity ◽  
Pcr Analysis ◽  
Uridine Diphosphate ◽  
Lipophilic Compounds ◽  
Isolation And Characterization ◽  
Hormonal Steroids

The present study reports the genomic organization and the characterization of a novel cynomolgus monkey UDP-glucuronosyltransferase (UGT) enzyme, UGT2B30. UGT enzymes are microsomal proteins that catalyse the transfer of the glucuronosyl group from UDP-glucuronic acid (UDPGA) to a wide variety of lipophilic compounds, namely hormonal steroids. The 15kb UGT2B30 gene amplified by PCR showed a genomic organization similar to those encoding UGT2B human enzymes. The cDNA encoding UGT2B30 was isolated from a cynomolgus monkey prostate cDNA library, and the deduced amino acid sequence showed an identity of 94% with UGT2B19, a monkey isoform previously characterized. Stable expression of UGT2B30 protein in human kidney 293 (HK293) cells was assessed by Western-blot analysis and its conjugating activity was screened using 39 potential substrates. The UGT2B30 enzyme is active on many compounds of different classes, including testosterone, dihydrotestosterone, 5α-androstane-3α,17β-diol, androsterone, oestradiol, tetrahydroaldosterone and tetrahydrocortisone, with glucuronidation efficiencies (Vmax/Km ratios) ranging from 0.6 to 8.8μl·min−1·mg of protein−1. Reverse-transcriptase-PCR analysis revealed that the UGT2B30 transcript is expressed in several tissues, including prostate, testis, mammary gland, kidney, adrenals and intestine. The relative activity of UGT2B30 in comparison with other simian UGT2B isoforms, as well as its large variety of substrates, strongly suggest that this enzyme is essential to inactivation of several steroids.

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