scholarly journals Characterization of the UDP-glucuronosyltransferase isoenzyme expressed in rat ovary and its regulation by gonadotropins

1998 ◽  
Vol 332 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Luisa BECEDAS ◽  
Bo LUNDGREN ◽  
Joseph W. De PIERRE
Keyword(s):  
Luteinizing Hormone ◽  
Chorionic Gonadotropin ◽  
Follicle Stimulating Hormone ◽  
Immature Rat ◽  
Immature Rats ◽  
Serum Gonadotropin ◽  
Immunoreactive Band ◽  
Rat Ovary ◽  
Udp Glucuronosyltransferase

Earlier studies have demonstrated that phenol UDP-glucuronosyltransferase (UGT) activity is up-regulated by pregnant mare's serum gonadotropin (PMSG) in rat ovary, but not liver. This phenomenon was investigated in more detail in the present study. Ovaries and livers of immature rats, rats synchronized with respect to their preovulatory and corpus lutealphases by treatment with PMSG, and mature rats hyperstimulated with PMSG were compared. Under all of these conditions, only one immunoreactive band of UGT, shown to be phenol UGT, was detected in the rat ovary. The effects of oestradiol, progesterone and/or human chorionic gonadotropin (hCG) on the level of phenol UGT in immature rat ovary were also examined. Partial up-regulation was caused by progesterone or oestradiol, together with hCG, whereas progesterone or oestradiol alone had no up-regulating effect. Follicle-stimulating hormone also seemed to be required for the up-regulation in ovaries enriched in corpus luteum. The present findings demonstrate that progesterone is involved in the regulation of phenol UGT in rat ovary by gonadotropins. Regulation by both progesterone and oestradiol was dependent on induction of ovulation and steroidogenesis by luteinizing hormone.

FOLLICLE STIMULATING HORMONE (FSH) AND LUTEINIZING HORMONE-HUMAN CHORIONIC GONADOTROPIN (LH-HCG) CONCENTRATIONS IN PAIRED MATERNAL AND CORD SERA

PEDIATRICS ◽  
1974 ◽  
Vol 53 (1) ◽  
pp. 41-47
Author(s):  
Robert Penny ◽  
N. Olatunji Olambiwonnu ◽  
S. Douglas Frasier
Keyword(s):  
Luteinizing Hormone ◽  
Chorionic Gonadotropin ◽  
Follicle Stimulating Hormone ◽  
Poor Correlation ◽  
Male Fetus ◽  
Gonadotropin Secretion ◽  
Female Fetus ◽  
Cord Serum ◽  
Serum Gonadotropin ◽  
Radio Immunoassay

FSH and LH-HCG concentrations were determined by radio-immunoassay in paired maternal and cord sera. The sera of 50 mothers and 51 infants, 25 female and 26 male (one set of twins), were assayed. Mean (± SD) FSH concentration of mothers (3.4 ± 0.9 mIU/ml) giving birth to female infants was not different, p>0.1, from that of mothers (3.7 ± 0.7 mIU/ml) giving birth to male infants. In contrast, mean (± SD) LH-HCG concentration of mothers (15.99 ± 3.1 IU/ml) giving birth to female infants was significantly, p<0.005, greater than that of mothers (11.37 ± 5.0 IU/ml) giving birth to male infants. Cord serum FSH mean (± SD) concentration was significantly, p<0.005, greater in female infants (3.7 ± 0.5 mIU/ml) than male infants (2.4 ± 0.8 mIU/ml). However, mean (± SD) LH-HCG concentrations in female infants (0.122 ± 0.015 IU/ml) was significantly, p<0.005, less than that of male infants (0.156 ± 0.040 IU/ml). Poor correlation, on an individual basis and on a statistical basis, between maternal and cord serum FSH and LH-HCG concentrations was observed. The data of this investigation are consistent with fetal pituitary gonadotropin secretion, confirm previous observations that women bearing a female fetus have higher LH-HCG concentrations than those bearing a male fetus, and suggest a sex difference in cord serum gonadotropin concentrations.

EFFECT OF SERUM GONADOTROPHIN ON DEHYDROGENASES OF THE CITRIC ACID CYCLE IN THE OVARY OF THE RAT

1963 ◽  
Vol 42 (4) ◽  
pp. 480-484 ◽  
Author(s):  
B. Eckstein ◽  
R. Landsberg
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Age Groups ◽  
Succinic Dehydrogenase ◽  
Immature Rat ◽  
Acid Cycle ◽  
Immature Rats ◽  
Rat Ovary

ABSTRACT The succinic, malic and isocitric dehydrogenases in the ovary of immature and mature, normal and serum gonadotrophin injected rats were examined. The Qo2 of these enzymes were markedly enhanced in the gonadotrophin injected rats of both age groups, except in the case of succinic dehydrogenase in the ovary of the immature rats, where a slight non-significant decrease was noted. It is concluded that in the mature rat ovary, gonadotrophin administration stimulates the activity of all the examined dehydrogenases of the citric acid cycle, whereas in the immature rat ovary, at least the isocitric- and malic dehydrogenases are thus stimulated.

FOLLICLE STIMULATING HORMONE (FSH) AND LUTEINIZING HORMONE-HUMAN CHORIONIC GONADOTROPIN (LH-HCG) CONCENTRATIONS IN PAIRED MATERNAL AND CORD SERA

PEDIATRICS ◽  
1974 ◽  
Vol 54 (1) ◽  
pp. 42-42
Author(s):  
Robert Penny ◽  
N. Olatunji Olambiwonnu ◽  
S. Douglas Frasier
Keyword(s):  
Luteinizing Hormone ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Follicle Stimulating Hormone ◽  
January Issue ◽  
Stimulating Hormone

Penny, R., Olambiwonnu, N. O., and Frasier, S. D.: Follicle stimulating hormone (FSH) and luteinizing hormone-human chorionic gonadotropin (LH-HCG) concentrations in paired maternal and cord sera. Pediatrics, 53:41, 1974. An error was made by the authors under the Materials and Methods section of the above article which appeared in the January issue. The last two sentences should read: To convert to nanograms of LER 907, multiply FSH concentrations by 24 and LH concentrations by 17. For conversion to International Units of commercial HCG, divide LH-HCG concentrations by 0.55.

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