scholarly journals A cDNA cloned from pregnant mouse uterus exhibits temporo-spatial expression and predicts a novel protein

1998 ◽  
Vol 330 (2) ◽  
pp. 947-950 ◽  
Author(s):  
W. John KASIK
Keyword(s):  
Late Pregnancy ◽  
Pregnant Mouse ◽  
Open Reading Frame ◽  
Mouse Uterus ◽  
Pregnant Uterus ◽  
Reading Frame ◽  
Spatial Expression ◽  
Conceptual Translation ◽  
Novel Protein ◽  
Specific Mrna

A cDNA was cloned from a pregnant mouse uterus cDNA library. On conceptual translation, the cDNA has one long open reading frame that predicts a novel protein of 606 amino acids. This protein is principally composed of two CUB domains and a ZP domain; motifs found in proteins implicated in egg-sperm recognition. Probes derived from the cDNA were used to conduct Northern hybridizations. The expression of this mRNA is temporal; message first appears in the uterus 6 days prior to birth, it increases each subsequent day to attain maximal levels at 3 days prior to birth and then abruptly decreases during the last 3 days of pregnancy. The expression of this mRNA is restricted; message is abundant in the uterus during late pregnancy, but it is not found in non-pregnant uterus or in a variety of adult or fetal tissues. The temporo-spatial expression of this pregnant uterus specific mRNA and the consolidation in the predicted protein of two motifs implicated in early pregnancy events suggests that the product of the gene represented by this mRNA may play an important role in events that transpire during late pregnancy.

scholarly journals Hypercholesterolemia impairs oxytocin-induced uterine contractility in late pregnant mouse

Reproduction ◽  
2017 ◽  
Vol 153 (5) ◽  
pp. 565-576 ◽  
Author(s):  
Amol R Padol ◽  
Susanth V Sukumaran ◽  
Abdul Sadam ◽  
Manickam Kesavan ◽  
Kandasamy Arunvikram ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Ex Vivo ◽  
Late Pregnancy ◽  
Sodium Cholate ◽  
Pregnant Mouse ◽  
Standard Diet ◽  
Uterine Contractility ◽  
High Cholesterol ◽  
Mouse Uterus

High cholesterol is known to negatively affect uterine contractility inex vivoconditions. The aim of the present study was to reveal the effect ofin vivohypercholesterolemia on spontaneous and oxytocin-induced uterine contractility in late pregnant mouse uterus. Female Swiss albino mice were fed with high cholesterol (HC) diet (0.5% sodium cholate, 1.25% cholesterol and 15% fat) for 6 weeks and then throughout the gestation period after mating. On day 19 of gestation, serum cholesterol level was increased more than 3-fold while triglycerides level was reduced in HC diet-fed animals as compared to control animals fed with a standard diet. In tension experiments, neither the mean integral tension of spontaneous contractility nor the response to CaCl2in high K+-depolarized tissues was altered, but the oxytocin-induced concentration-dependent contractile response in uterine strips was attenuated in hypercholesterolemic mice as compared to control. Similarly, hypercholesterolemia dampened concentration-dependent uterine contractions elicited by a GNAQ protein activator,Pasteurella multocidatoxin. However, it had no effect on endogenous oxytocin level either in plasma or in uterine tissue. It also did not affect the prostaglandin release in oxytocin-stimulated tissues. Western blot data showed a significant increase in caveolin-1 and GRK6 proteins but decline in oxytocin receptor, GNAQ and RHOA protein expressions in hypercholesterolemic mouse uterus. The results of the present study suggest that hypercholesterolemia may attenuate the uterotonic action of oxytocin in late pregnancy by causing downregulation of oxytocin receptors and suppressing the signaling efficacy through GNAQ and RHOA proteins.

scholarly journals β-Catenin (CTNNB1) in the Mouse Uterus During Decidualization and the Potential Role of Two Pathways in Regulating Its Degradation

2007 ◽  
Vol 55 (9) ◽  
pp. 963-974 ◽  
Author(s):  
Jennifer L. Herington ◽  
JiaJia Bi ◽  
John D. Martin ◽  
Brent M. Bany
Keyword(s):  
Binding Protein ◽  
E3 Ligase ◽  
Progressive Increase ◽  
Pregnant Mouse ◽  
Transcriptional Coactivator ◽  
Mouse Uterus ◽  
Endometrial Stromal Cells ◽  
Pregnant Uterus ◽  
Steady State Levels

β-catenin plays a role in cell adhesion and as a transcriptional coactivator. Its levels are regulated in cells by controlling its degradation through ubiquitination by two different E3 ligase complexes. One complex contains β-transducing repeat containing (BTRC) protein, which binds to β-catenin when phosphorylated on specific (S33 and S37) residues, whereas the other involves calcyclin-binding protein (CACYBP). The aim of this study was to determine the localization and levels of total and active (S33/S37-dephosphorylated) β-catenin in the pregnant mouse uteri and those undergoing artificially stimulated decidualization. These two forms of β-catenin were localized almost exclusively to the endometrial epithelia just prior to the onset of implantation. Although this localization continued after the onset of implantation, there were less epithelial cells present in areas of the uterus undergoing decidualization. Rather, there was a progressive increase in β-catenin localization in endometrial stromal cells undergoing decidualization in the anti-mesometrial and, to a lesser extent, in the mesometrial regions. The presence of a conceptus was not required for the changes in localization seen in the pregnant uterus because similar findings were also seen in uteri undergoing artificially stimulated decidualization. Finally, overall levels of total, active (S33 and S37 dephosphorylated), and phosphorylated (S33/S37/T42) β-catenin protein and the steady-state levels of calcyclin-binding protein mRNA changed in the uterus during decidualization. The result of this study shows the changing localization and levels of β-catenin in the mouse uterus during decidualization. Further, the results suggest potential roles for both the BTRC and CACYBP E3 ligase mechanisms of β-catenin ubiquitination in the uterus during decidualization.

The Drosophila gene asteroid encodes a novel protein and displays dosage-sensitive interactions with Star and Egfr

Genome ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 295-302 ◽  
Author(s):  
Michael A Kotarski ◽  
Deborah A Leonard ◽  
Sean A Bennett ◽  
Clifton P Bishop ◽  
Stephen D Wahn ◽  
...  
Keyword(s):  
Amino Acids ◽  
Egf Receptor ◽  
Open Reading Frame ◽  
Cdna Clones ◽  
Putative Open Reading Frame ◽  
Morphogenetic Furrow ◽  
Calculated Molecular Mass ◽  
Reading Frame ◽  
Ribonuclease Protection ◽  
Novel Protein

The asteroid gene of Drosophila was found to lie within 189 bp of Star. Asteroid cDNA clones were isolated and sequenced and a single putative open reading frame was identified that encodes a novel protein of 815 amino acids with a calculated molecular mass of 93 kilodaltons. Using cDNA probes, asteroid transcripts were localized to the proliferative tissues of embryos and to the mitotically active tissue anterior to the morphogenetic furrow in eye imaginal discs. Ribonuclease protection assays identified a mutation of asteroid that acts as a dominant enhancer of Star mutations and also enhances the Ellipse mutation, EgfrE1. Based on these data, a model for asteroid gene function in EGF receptor signaling is presented.Key words: Drosophila, asteroid, Star, EGF receptor, eye development.

ORIGINAL ARTICLE: Temporal and Spatial Expression of Tumor-Associated Antigen RCAS1 in Pregnant Mouse Uterus

2009 ◽  
Vol 63 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Ekaterine Tskitishvili ◽  
Hitomi Nakamura ◽  
Yukiko Kinugasa-Taniguchi ◽  
Takeshi Kanagawa ◽  
Tadashi Kimura ◽  
...  
Keyword(s):  
Pregnant Mouse ◽  
Mouse Uterus ◽  
Spatial Expression ◽  
Tumor Associated Antigen ◽  
Temporal And Spatial

scholarly journals A Genomic Approach to Identify Novel Progesterone Receptor Regulated Pathways in the Uterus during Implantation

2002 ◽  
Vol 16 (12) ◽  
pp. 2853-2871 ◽  
Author(s):  
Yong-Pil Cheon ◽  
Quanxi Li ◽  
Xueping Xu ◽  
Francesco J. DeMayo ◽  
Indrani C. Bagchi ◽  
...  
Keyword(s):  
Gene Networks ◽  
Time Course ◽  
Target Genes ◽  
Reproductive Tract ◽  
Cell Types ◽  
Temporal Analysis ◽  
Pregnant Mouse ◽  
Initial Examination ◽  
Mouse Uterus ◽  
Pregnant Uterus

Abstract The cellular actions of steroid hormone progesterone (P) are mediated via its nuclear receptors, which regulate the expression of specific target genes. The identity of gene networks that are regulated by the P receptors (PRs) in the uterus at various stages of the reproductive cycle and pregnancy, however, remain largely unknown. In this study, we have used oligonucleotide microarrays to identify mRNAs whose expression in the pregnant mouse uterus is modulated by RU486, a well-characterized PR antagonist, which is also an effective inhibitor of implantation. We found that, in response to RU486, expression of mRNAs corresponding to 78 known genes was down-regulated at least 2-fold in the preimplantation mouse uterus. The PR regulation of several of these genes was ascertained by administering P to ovariectomized wild-type and PR knockout (PRKO) mice. Detailed spatio-temporal analysis of these genes in the pregnant uterus indicated that their expression in the epithelium and stroma could be correlated with the expression of PR in those cell types. Furthermore, time-course studies suggested that many of these genes are likely primary targets of PR regulation. We also identified 70 known genes that were up-regulated at least 2-fold in the pregnant uterus in response to RU486. Interestingly, initial examination of a number of RU486-inducible genes reveals that their uterine expression is also regulated by estrogen. The identification of several novel PR-regulated gene pathways in the reproductive tract is an important step toward understanding how P regulates the physiological events leading to implantation.

Molecular Analysis of EMS-Induced frizzled Mutations in Drosophila melanogaster

Genetics ◽  
1996 ◽  
Vol 142 (1) ◽  
pp. 205-215 ◽  
Author(s):  
Katherine H Jones ◽  
Jingchun Liu ◽  
P N Adler
Keyword(s):  
Amino Acids ◽  
Drosophila Melanogaster ◽  
Membrane Protein ◽  
Western Blot ◽  
Normal Tissue ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Tissue Polarity ◽  
Sds Page ◽  
Conceptual Translation

The frizzled (fz) gene of Drosophila is essential for the development of normal tissue polarity in the adult cuticle of Drosophila. In fz mutants the parallel array of hairs and bristles that decorate the cuticle is disrupted. Previous studies have shown that fz encodes a membrane protein with seven putative transmembrane domains, and that it has a complex role in the development of tissue polarity, as there exist both cell-autonomous and cell nonautonomous alleles. We have now examined a larger number of alleles and found that 15 of 19 alleles display cell nonautonomy. We have examined these and other alleles by Western blot analysis and found that most fz mutations result in altered amounts of Fz protein, and many also result in a Fz protein that migrates aberrantly in SDS-PAGE. We have sequenced a subset of these alleles. Cell nonautonomous fz alleles were found to be associated with mutations that altered amino acids in all regions of the Fz protein. Notably, the four cell-autonomous mutations were all in a proline residue located in the presumptive first cytoplasmic loop of the protein. We have also cloned and sequenced the fz gene from D. virilis. Conceptual translation of the D. virilis open reading frame indicates that the Fz protein is unusually well conserved. Indeed, in the putative cytoplasmic domains the Fz proteins of the two species are identical.

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