scholarly journals Binding of glutathione and an inhibitor to microsomal glutathione transferase

1997 ◽  
Vol 326 (1) ◽  
pp. 193-196 ◽  
Author(s):  
Tie-Hua SUN ◽  
Ralf MORGENSTERN
Keyword(s):  
Glutathione Transferase ◽  
Equilibrium Dialysis ◽  
Kinetic Studies ◽  
Phase System ◽  
Liver Protein ◽  
Two Phase ◽  
Equilibrium Partition ◽  
Binding Isotherms ◽  
Equilibrium Binding Constant ◽  
Microsomal Glutathione

Microsomal glutathione transferase is an abundant liver protein that can be activated by thiol reagents. It is not known whether the activation is associated with changed binding properties of the enzyme. Therefore the binding of GSH and an inhibitor to rat liver microsomal glutathione transferase was studied by use of equilibrium dialysis and equilibrium partition in a two-phase system. The radioactive substrate glutathione and an inhibitor (glutathione sulphonate) give hyperbolic binding isotherms with a stoichiometry of 1 mol per mol of enzyme (i.e. 1 molecule per homotrimer). Glutathione had an equilibrium binding constant of 18 μM. Competition experiments involving glutathione sulphonate showed that it could effectively displace GSH. These and kinetic studies showed that the Kd and Ki for glutathione sulphonic acid are close to 10 μM. No change in these parameters was obtained after N-ethylmaleimide activation of the enzyme. Thus activation does not result from changes in binding affinity to GSH.

Kinetic studies on rat liver microsomal glutathione transferase: consequences of activation

1995 ◽  
Vol 1247 (2) ◽  
pp. 277-283 ◽  
Author(s):  
Claes Andersson ◽  
Fiorella Piemonte ◽  
Erifili Mosialou ◽  
Rolf Weinander ◽  
Tie-Hua Sun ◽  
...  
Keyword(s):  
Rat Liver ◽  
Glutathione Transferase ◽  
Kinetic Studies ◽  
Microsomal Glutathione

On the Problem of “Two-Phase” Activated Sludge

1991 ◽  
Vol 24 (7) ◽  
pp. 59-64 ◽  
Author(s):  
R. W. Szetela
Keyword(s):  
Activated Sludge ◽  
Single Phase ◽  
Phase System ◽  
Two Phase ◽  
Wastewater Treatment Process ◽  
Sludge Stabilization ◽  
Technological Advantage ◽  
In Series ◽  
State Models ◽  
Activated Sludge Systems

Steady-state models are presented to describe the wastewater treatment process in two activated sludge systems. One of these makes use of a single complete-mix reactor; the other one involves two complete-mix reactors arranged in series. The in-series system is equivalent to what is known as the “two-phase” activated sludge, a concept which is now being launched throughout Poland in conjunction with the PROMLECZ technology under implementation. Analysis of the mathematical models has revealed the following: (1) treatment efficiency, excess sludge production, energy consumption, and the degree of sludge stabilization are identical in the two systems; (2) there exists a technological equivalence of “two-phase” sludge with “single-phase” sludge; (3) the “two-phase” system has no technological advantage over the “single-phase” system.

Interphase distribution of 2-furylethylenes

1985 ◽  
Vol 50 (8) ◽  
pp. 1642-1647 ◽  
Author(s):  
Štefan Baláž ◽  
Anton Kuchár ◽  
Ernest Šturdík ◽  
Michal Rosenberg ◽  
Ladislav Štibrányi ◽  
...  
Keyword(s):  
Partition Coefficient ◽  
Partition Coefficients ◽  
Transport Rate ◽  
Phase System ◽  
Two Phase ◽  
Interphase Distribution ◽  
Distribution Kinetics ◽  
System 1 ◽  
Kinetics Of

The distribution kinetics of 35 2-furylethylene derivatives in two-phase system 1-octanol-water was investigated. The transport rate parameters in direction water-1-octanol (l1) and backwards (l2) are partition coefficient P = l1/l2 dependent according to equations l1 = logP - log(βP + 1) + const., l2 = -log(βP + 1) + const., const. = -5.600, β = 0.261. Importance of this finding for assesment of distribution of compounds under investigation in biosystems and also the suitability of the presented method for determination of partition coefficients are discussed.

Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System

2021 ◽  
Author(s):  
Qiaoshu Chen ◽  
Yanwen Zhang ◽  
Hui Chen ◽  
Jianbo Liu ◽  
Juewen Liu
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Development of an ethanolic two-phase system (ETPS) based on polypropylene glycol 2000 + ethylene glycol + ethanol for separation of hydrophobic compounds

2021 ◽  
Author(s):  
Filipe Smith Buarque ◽  
Cleide Mara Faria Soares ◽  
Ranyere Lucena de Souza ◽  
Matheus Mendonça Pereira ◽  
Álvaro Silva Lima
Keyword(s):  
Ethylene Glycol ◽  
Phase System ◽  
Polypropylene Glycol ◽  
Two Phase ◽  
Hydrophobic Compounds ◽  
Ethanol Content ◽  
Coexisting Phases ◽  
Phase Water ◽  
High Ethanol

Two-phase water-free systems containing high ethanol content in the coexisting phases can selectively partition hydrophobic molecules from natural biomass.

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