scholarly journals Characterization of a partially structured state in an all-β-sheet protein

1997 ◽  
Vol 321 (2) ◽  
pp. 457-464 ◽  
Author(s):  
Thirunavukkarasu SIVARAMAN ◽  
Thallampuranam K. S. KUMAR ◽  
Gurunathan JAYARAMAN ◽  
Chien C. HAN ◽  
Chin YU
Keyword(s):  
Trichloroacetic Acid ◽  
Molten Globule ◽  
Stable State ◽  
Two Dimensional ◽  
Fourier Transform Ir ◽  
Fluorescence Binding ◽  
Ctx Iii ◽  
Β Sheet ◽  
Naja Atra

Cardiotoxin analogue III (CTX III) is a low-molecular-mass all-α-sheet protein isolated from the Taiwan cobra (Naja naja atra) venom. A stable partially structured state similar to the ‘molten globule’ state has been identified for CTX III in a 3% (w/v) solution of 2,2,2-trichloroacetic acid at 298 K. This stable state has been structurally characterized using a variety of techniques such as CD, 1-anilinonaphthalene-8-sulphonate fluorescence binding, Fourier transform IR and two-dimensional NMR spectroscopy techniques. Direct assignment of the homonuclear two-dimensional NMR spectra of the protein in 3% trichloroacetic acid showed that drastic structural perturbation had not taken place in the protein and that the ‘intermediate’ state retained a significant portion of the native secondary-structural interactions. It is found that about 65% of the native α-sheet structural contacts are maintained in the partially structured state of CTX III in 3% trichloroacetic acid.

scholarly journals Identification of a α‐helical molten globule intermediate and structural characterization of β‐cardiotoxin, an all β‐sheet protein isolated from the venom ofOphiophagus hannah(king cobra)

2019 ◽  
Vol 28 (5) ◽  
pp. 952-963 ◽  
Author(s):  
Amrita Roy ◽  
Sun Qingxiang ◽  
Chapeaurouge Alex ◽  
Nandhakishore Rajagopalan ◽  
Chacko Jobichen ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Molten Globule ◽  
Β Sheet ◽  
King Cobra

Characterization of compounds in crude oils by gas purge micro-syringe extraction coupled to comprehensive two-dimensional gas chromatography

2014 ◽  
Vol 32 (10) ◽  
pp. 1144
Author(s):  
Ting TONG ◽  
Wanfeng ZHANG ◽  
Donghao LI ◽  
Jinhua ZHAO ◽  
Zhenyang CHANG ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Crude Oils ◽  
Two Dimensional ◽  
Gas Purge

scholarly journals Terahertz characterization of two-dimensional low-conductive layers enabled by metal gratings

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Prashanth Gopalan ◽  
Yunshan Wang ◽  
Berardi Sensale-Rodriguez
Keyword(s):  
Optical Transmission ◽  
Terahertz Spectroscopy ◽  
Design Guidelines ◽  
Two Dimensional ◽  
Highly Sensitive ◽  
Transmission Measurements ◽  
Metal Gratings ◽  
Low Sensitivity ◽  
Non Destructive

AbstractWhile terahertz spectroscopy can provide valuable information regarding the charge transport properties in semiconductors, its application for the characterization of low-conductive two-dimensional layers, i.e., σs <  < 1 mS, remains elusive. This is primarily due to the low sensitivity of direct transmission measurements to such small sheet conductivity levels. In this work, we discuss harnessing the extraordinary optical transmission through gratings consisting of metallic stripes to characterize such low-conductive two-dimensional layers. We analyze the geometric tradeoffs in these structures and provide physical insights, ultimately leading to general design guidelines for experiments enabling non-contact, non-destructive, highly sensitive characterization of such layers.

Mechanics of free-standing inorganic and molecular 2D materials

Nanoscale ◽  
2021 ◽  
Author(s):  
Xianghui Zhang ◽  
Andre Beyer
Keyword(s):  
Recent Progress ◽  
Mechanical Characterization ◽  
2D Materials ◽  
Two Dimensional ◽  
Free Standing ◽  
Inorganic As

The discovery of graphene has triggered a great interest in inorganic as well as molecular two-dimensional (2D) materials. In this review, we summarize recent progress in the mechanical characterization of...

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