scholarly journals Structural studies on a lipoarabinogalactan of Crithidia fasciculata

1996 ◽  
Vol 313 (3) ◽  
pp. 963-971 ◽  
Author(s):  
Pascal SCHNEIDER ◽  
Achim TREUMANN ◽  
Kenneth G. MILNE ◽  
Malcolm J. McCONVILLE ◽  
Nicole ZITZMANN ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Mass Spectrometry ◽  
Leishmania Major ◽  
Hydrophobic Interaction Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Structural Studies ◽  
Donor Molecule ◽  
Membrane Anchor ◽  
Crithidia Fasciculata ◽  
Trypanosomatid Parasites

The monosaccharide D-arabinopyranose has only been found in glycoconjugates of the trypanosomatid parasites Leishmania major, Endotrypanum schaudinni and Crithidia fasciculata. The donor molecule for the relevant arabinosyltransferases is known to be GDP-α-D-Arap in L. major and C. fasciculata, and the latter organism is being used to study the biosynthesis of GDP-α-D-Arap. In this study, we describe the structure of the terminal product of arabinose metabolism in C. fasciculata, namely lipoarabinogalactan. This molecule was purified by hydrophobic-interaction chromatography and studied by a variety of techniques, including gas chromatography–mass spectrometry, electrospray mass spectrometry and chemical and enzymic digestions. These data show that lipoarabinogalactan contains a previously described D-arabino-D-galactan polysaccharide component covalently attached to a glycosylphosphatidylinositol type of membrane anchor that is similar to, but not identical with, that found in the lipophosphoglycans of the Leishmania.

scholarly journals Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase

1994 ◽  
Vol 302 (3) ◽  
pp. 861-865 ◽  
Author(s):  
C A Redman ◽  
J E Thomas-Oates ◽  
S Ogata ◽  
Y Ikehara ◽  
M A Ferguson
Keyword(s):  
Mass Spectrometry ◽  
Alkaline Phosphatase ◽  
Fast Atom Bombardment ◽  
Compositional Analysis ◽  
Hydrophobic Interaction Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Placental Alkaline Phosphatase ◽  
Membrane Anchor ◽  
Human Placental Alkaline Phosphatase ◽  
Ethanolamine Phosphate

The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycosylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety was analysed by fast-atom bombardment mass spectrometry and gas chromatography-mass spectrometry. Taken together the data suggest the structure, Thr-Asp-ethanolamine-PO4-Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN-(sn-1-O- alkyl-2-O-acylglycerol-3-PO4-1-myo-D-inositol), which contains an additional ethanolamine phosphate group at an unknown position.

scholarly journals The glycoinositol-phospholipids of Phytomonas

1995 ◽  
Vol 311 (2) ◽  
pp. 495-503 ◽  
Author(s):  
C A Redman ◽  
P Schneider ◽  
A Mehlert ◽  
M A J Ferguson
Keyword(s):  
Mass Spectrometry ◽  
Cell Surface ◽  
Chemical Structure ◽  
Electrospray Mass Spectrometry ◽  
Water Soluble ◽  
Cell Surface Molecules ◽  
Surface Molecules ◽  
Euphorbia Characias ◽  
Trypanosomatid Parasites ◽  
Glycolipid Fraction

The Phytomonas spp. are trypanosomatid parasites of plants. A polar glycolipid fraction of a Phytomonas sp., isolated from the plant Euphorbia characias and grown in culture, was fractionated into four major glycolipid species (Phy 1-4). The glycolipids were analysed by chemical and enzymic modifications, composition and methylation analyses, electrospray mass spectrometry and microsequencing after HNO2 deamination and NaB3H4 reduction. The water-soluble headgroup of the Phy2 glycolipid was also analysed by 1H NMR. All four glycolipids were shown to be glycoinositol-phospholipids (GIPLs) with phosphatidylinositol (PI) moieties containing the fully saturated alkylacylglycerol lipids 1-O-hexadecyl-2-O-palmitoylglycerol and 1-O-hexadecyl-2-O-stearoylglycerol. The structures of the Phy 1-4 GIPLs are: Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6PI, Glc alpha 1-2(NH2-CH2CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6PI, [formula: see text] Glc alpha 1-2(NH2CH2CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4(NH2-CH2CH2-HPO4-)GlcN alpha 1-6PI [formula: see text] and Glc alpha 1-2Glc alpha 1-2(NH2CH2-CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4(NH2CH2CH2-HPO4-)-GlcN alpha 1-6PI. [formula: see text] The Phytomonas GIPLs represent a novel series of structures. This is the first description of the chemical structure of cell-surface molecules of this plant pathogen. The Phytomonas GIPLs are compared with those of other trypanosomatid parasites and are discussed with respect to trypanosomatid phylogenetic relationships.

Synthesis and Structural Studies of Tetrahedral [M(Ph2P(CH=CH)PPh2)2]BPh4 and [M(Ph2P(CH2)nPPh2)2]BPh4 (n = 2, 3) Complexes for M = Copper(I), Silver(I), and Gold(I)

2012 ◽  
Vol 65 (7) ◽  
pp. 811 ◽  
Author(s):  
Peter C. Healy ◽  
Bradley T. Loughrey ◽  
Michael L. Williams
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Structural Analysis ◽  
Single Crystal ◽  
Copper Complex ◽  
Electrospray Mass Spectrometry ◽  
Structural Studies ◽  
Space Group ◽  
X Ray ◽  
Bond Lengths

Tetraphenylborate salts of the 1 : 2 tetrahedral bis(bidentate) complexes of copper(i), silver(i), and gold(i) with cis-bis(diphenylphosphino)ethene (dppey), [M(Ph2PCH=CHPPh2)2]BPh4, cis-bis(diphenylphosphino)ethane (dppe), and cis-bis(diphenylphosphino)propane (dppp), [M(Ph2P–R–PPh2)2]BPh4 (R = (CH2)2, (CH2)3), have been prepared and characterized by NMR spectroscopy, electrospray mass spectrometry, and single crystal X-ray structural analysis at 200 K. Structures of the dppey complexes as acetone solvates show the Cu and Ag complexes to be isostructural with the previously reported Au complex, crystallizing in space group P 21/n with a ~ 25.5, b ~ 15.6, and c ~ 34.2 Å, and β ~ 103°. Structures of the dppe complexes show the copper complex to crystallize in the chiral space group P21 with a 11.8954(5), b 20.1904(5), and c 14.8999(5) Å, and β 111.366(5)°. The Ag and Au complexes are isostructural, crystallizing in space group P21/c with a ~ 17.8, b ~ 17.6, and c ~ 20.9 Å, and β ~ 107°. Structures of the dppp complexes show the copper complex to crystallize in space group P21/c with a 17.8399(4), b 19.8803(4), and c 18.7165(3) Å, and β 102.197(2)°. The Ag and Au complexes are isostructural, crystallizing in space group P21/n with a ~ 17.7, b ~ 18.6, and c ~ 19.6 Å, and β ~ 100°. Across the three series of complexes, the M–P bond lengths increase in the order Cu < Au < Ag with Cu–P = 2.281(3)–2.314(1) Å, average 2.30(1) Å; Au–P = 2.363(1)–2.4322(8) Å, average 2.40(2) Å, and Ag–P = 2.445(1)–2.5303(5) Å average 2.49(2) Å.

The Application of Electrospray Mass Spectrometry for Structural Studies on a Tetrasaccharide Monopeptide from the Urine of a Patient with α-N-Acetylhexosaminidase Deficiency

1994 ◽  
Vol 13 (3) ◽  
pp. 447-456 ◽  
Author(s):  
Jasna Peter-Kataliníc ◽  
Kerstin Williger ◽  
Heinz Egge ◽  
Brian Green ◽  
Franz-Georg Hanisch ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Mass Spectrometry ◽  
Structural Studies
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