scholarly journals Analysis of the mitochondrial ATP synthase β-subunit gene in Drosophilidae: structure, transcriptional regulatory features and developmental pattern of expression in Drosophila melanogaster

1995 ◽  
Vol 312 (3) ◽  
pp. 887-897 ◽  
Author(s):  
P Peña ◽  
C Ugalde ◽  
M Calleja ◽  
R Garesse
Keyword(s):  
Atp Synthase ◽  
Amino Acid Level ◽  
Regulatory Elements ◽  
Beta Subunit ◽  
Drosophila Species ◽  
Leader Peptide ◽  
Nucleotide Sequence Analysis ◽  
Promoter Regions ◽  
Gene Encoding ◽  
Mitochondrial Atp Synthase

We have cloned and determined the structure of the gene encoding the H(+)-ATP synthase beta subunit in two distantly related Drosophila species, D. melanogaster and D. virilis. The gene contains three exons that are extremely well conserved at the amino acid level, not only in the region encoding the mature protein but also in that encoding the leader peptide. Primer extension analysis indicates that the 5′ untranslated region is extremely short, and reveals the presence of multiple initiation sites of transcription in both Drosophila species. The promoters of D. melanogaster and D. virilis H(+)-ATP synthase beta-subunit genes contain a conserved region surrounding the initiation transcription sites. Nucleotide sequence analysis has revealed the absence of canonical TATA and CCAAT boxes and the presence of several putative regulatory elements in both promoter regions, including GAGA, GATA and Ets binding sites. We have analysed the pattern of gene expression during D. melanogaster development. The mRNA is stored in oocytes, and activation of transcription takes place after 10 h of development. The expression of the nuclear-encoded H(+)-ATP synthase beta subunit is strictly coordinated with the expression of subunits 6 and 8 of the same complex that are encoded in the mitochondrial genome.

scholarly journals Transport of the yeast ATP synthase β-subunit into mitochondria. Effects of amino acid substitutions on targeting

1990 ◽  
Vol 266 (1) ◽  
pp. 227-234 ◽  
Author(s):  
M E Walker ◽  
E Valentin ◽  
G A Reid
Keyword(s):  
Amino Acid ◽  
Atp Synthase ◽  
Beta Subunit ◽  
Growth Defect ◽  
Amino Acid Substitutions ◽  
Amino Acid Residues ◽  
Gene Encoding ◽  
Mitochondrial Atp Synthase

We have isolated the yeast ATP2 gene encoding the beta-subunit of mitochondrial ATP synthase and determined its nucleotide sequence. A fusion between the N-terminal 15 amino acid residues of beta-subunit and the mouse cytosolic protein dihydrofolate reductase (DHFR) was transcribed and translated in vitro and found to be transported into isolated yeast mitochondria. A fusion with the first 35 amino acid residues of beta-subunit attached to DHFR was not only transported but also proteolytically processed by a mitochondrial protease. Amino acid substitutions were introduced into the N-terminal presequence of the beta-subunit by bisulphite mutagenesis of the corresponding DNA. The effects of these mutations on mitochondrial targeting were assessed by transport experiments in vitro using DHFR fusion proteins. All of the mutants, harbourin from one to six amino acid substitutions in the first 14 residues of the presequence, were transported into mitochondria, though at least one of them (I8) was transported and proteolytically processed at a much reduced rate. The I8 mutant beta-subunit also exhibited poor transport and processing in vivo, and expression of this mutant polypeptide failed to complement the glycerol- phenotype of a yeast ATP2 mutant. More remarkably, the expression of I8 beta-subunit induced a more general growth defect in yeast, possibly due to interference with the transport of other, essential, mitochondrial proteins.

scholarly journals ETS transcription factors regulate the expression of the gene for the human mitochondrial ATP synthase beta-subunit.

1994 ◽  
Vol 269 (51) ◽  
pp. 32649-32654
Author(s):  
J.A. Villena ◽  
I. Martin ◽  
O. Viñas ◽  
B. Cormand ◽  
R. Iglesias ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Atp Synthase ◽  
Beta Subunit ◽  
Ets Transcription Factors ◽  
Mitochondrial Atp Synthase
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