scholarly journals Mouse gastric mucin: cloning and chromosomal localization

1995 ◽  
Vol 311 (3) ◽  
pp. 775-785 ◽  
Author(s):  
L L Shekels ◽  
C Lyftogt ◽  
M Kieliszewski ◽  
J D Filie ◽  
C A Kozak ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Dna Analysis ◽  
Sequence Similarity ◽  
Consensus Sequence ◽  
Repeat Sequence ◽  
Gastric Mucin ◽  
Gastric Epithelium ◽  
Chromosome 11P15 ◽  
Mucin Gene ◽  
Intestinal Mucin

Mucins protect gastric epithelium by maintaining a favourable pH gradient and preventing autodigestion. The purpose of this study was to clone a mouse gastric mucin which would provide a foundation for analysis of mucin gene regulation. Mucin was purified from the glandular portion of gastric specimens and deglycosylated by HF solvolysis. Antibodies against native and deglycosylated mouse gastric mucin (MGM) were raised in chickens. Screening of a mouse stomach cDNA library with the anti-(deglycosylated MGM) antibody yielded partial clones containing a 48 bp tandem repeat and 768 bp of non-repetitive sequence. The 16-amino-acid tandem repeat has a consensus sequence of QTSSPNTGKTSTISTT with 25% serine and 38% threonine. The MGM tandem repeat sequence bears no similarity to previously identified mucins. The MGM non-repetitive region shares sequence similarity with human MUC5AC and, to a lesser extent, human MUC2 and rat intestinal mucin. Northern blot analysis reveals a polydisperse message beginning at 13.5 kb in mouse stomach with no expression in oesophagus, trachea, small intestine, large intestine, caecum, lung or kidney. Immunoreactivity of antibodies against deglycosylated MGM and against a synthetic MGM tandem repeat peptide was restricted to superficial mucous cells, antral glands and Brunner's glands in the pyloric-duodenal region. DNA analysis shows that MGM recognizes mouse and rat DNA but not hamster, rabbit or human DNA. The MGM gene maps to a site on mouse chromosome 7 homologous to the location of a human secretory mucin gene cluster on human chromosome 11p15. Due to sequence similarity and predominant expression in the stomach, the MGM gene may be considered a MUC5AC homologue and named Muc5ac.

scholarly journals Isolation and characterization of cDNA clones encoding pig gastric mucin

1995 ◽  
Vol 308 (1) ◽  
pp. 89-96 ◽  
Author(s):  
B S Turner ◽  
K R Bhaskar ◽  
M Hadzopoulou-Cladaras ◽  
R D Specian ◽  
J T LaMont
Keyword(s):  
Amino Acid ◽  
Tandem Repeats ◽  
Consensus Sequence ◽  
Gastric Gland ◽  
Repeat Sequence ◽  
Gastric Mucin ◽  
Gastric Epithelium ◽  
Cdna Clones ◽  
Repeat Region ◽  
Isolation And Characterization

Polyclonal antibodies raised to deglycosylated pig gastric mucin were used to screen a cDNA library constructed with pig stomach mucosal mRNA. Immunocytochemistry indicated that the antibody recognizes intracellular and secreted mucin in surface mucous cells of pig gastric epithelium. A total of 70 clones producing proteins immunoreactive to this antibody were identified, two of which (PGM-2A,9B) were fully sequenced from both ends. Clone PGM-9B hybridized to a polydisperse mRNA (3-9 kb) from pig stomach, but not liver, intestine or spleen, nor to mRNA from human, mouse, rabbit or rat stomach. Sequence analysis indicated that PGM-9B encodes 33 tandem repeats of a 16-amino-acid consensus sequence rich in serine (46%) and threonine (17%). Using the restriction enzyme MwoI, which has a single target site in the repeat, it was demonstrated that PGM-9B consists entirely of this tandem repeat. Southern-blot analysis indicated that the repeat region is contained in a 20 kb HindIII-EcoRI fragment, and BamHI digestion suggested that most of the repeats are contained in a 10 kb fragment. In situ hybridization with an antisense probe to PGM-9B showed an intense signal in the entire gastric gland. Clone PGM-2A also contains the same repeat sequence as 9B, but, in addition, has a 64-amino-acid-long non-repeat region at its 5′ end. Interestingly the non-repeat region of PGM-2A has five cysteine residues, the arrangement of which is identical with that reported for human intestinal mucin gene MUC2.

Assignment of the polymorphic intestinal mucin gene (MUC2) to chromosome 11p15

1990 ◽  
Vol 54 (4) ◽  
pp. 277-285 ◽  
Author(s):  
B. GRIFFITHS ◽  
D. J. MATTHEWS ◽  
L. WEST ◽  
J. ATTWOOD ◽  
S. POVEY ◽  
...  
Keyword(s):  
Chromosome 11P15 ◽  
Mucin Gene ◽  
Intestinal Mucin

Human mucin gene MUC5AC: organization of its 5′-region and central repetitive region

2001 ◽  
Vol 358 (3) ◽  
pp. 763-772 ◽  
Author(s):  
Fabienne ESCANDE ◽  
Jean-Pierre AUBERT ◽  
Nicole PORCHET ◽  
Marie-Pierre BUISINE
Keyword(s):  
Central Region ◽  
Tandem Repeats ◽  
Sequence Similarity ◽  
Repetitive Region ◽  
High Sequence Similarity ◽  
Ancestral Gene ◽  
Chromosome 11P15 ◽  
Repeat Array ◽  
Mucin Gene ◽  
Von Willebrand

Human mucin gene MUC5AC is clustered with MUC2, MUC5B and MUC6 on chromosome 11p15.5. We report here the full length cDNA sequence upstream of the repetitive region of human MUC5AC. We have also determined the sequence of its large central tandem repeat array. The 5′-region reveals high degree of sequence similarity with MUC2 and MUC5B and codes for 1336 amino acids organized into a signal peptide, four pro-von Willebrand factor-like D domains (D1, D2, D′ and D3) and a short domain which connects to the central repetitive region. In the central region, 17 major domains have been identified. Nine code for cysteine-rich domains (Cys-domains 1–9) and exhibit high sequence similarity to the cysteine-rich domains described in the central region of MUC2 and MUC5B. Cys-domains 1–5 are interspersed by domains enriched with serine, threonine, and proline residues. Cys-domains 1–9 are interspersed by four domains (TR1–TR4) composed of various numbers of MUC5AC-type repeats. Southern-blot analyses reveal allelic variations both in length and nucleotide sequence. The length polymorphism which is due to variable numbers of tandem repeats is located in TR1 and TR4, whereas a mutation polymorphism detected with TaqI is located in Cys-domain 6. In this study, the organization of MUC5AC has been entirely elucidated showing extensive similarity to the other chromosome 11p15 MUC genes, particularly MUC5B, and providing additional arguments for common evolution from a single ancestral gene.

scholarly journals Immunological Dominance of Trypanosoma cruzi Tandem Repeat Proteins

2008 ◽  
Vol 76 (9) ◽  
pp. 3967-3974 ◽  
Author(s):  
Yasuyuki Goto ◽  
Darrick Carter ◽  
Steven G. Reed
Keyword(s):  
Trypanosoma Cruzi ◽  
Tandem Repeat ◽  
Transmembrane Domain ◽  
Signal Sequence ◽  
Sequence Similarity ◽  
Consensus Sequence ◽  
Host Susceptibility ◽  
Immunological Responses ◽  
Computational Screening ◽  
Tandem Repeat Proteins

ABSTRACT Proteins with tandem repeat (TR) domains have been found in various protozoan parasites, often acting as targets of B-cell responses. However, the extent of the repeats within Trypanosoma cruzi, the causative agent of Chagas’ disease, has not been examined well. Here, we present a systematic survey of the TR genes found in T. cruzi, in comparison with other organisms. Although the characteristics of TR genes varied from organism to organism, the presence of genes having large TR domains was unique to the trypanosomatids examined, including T. cruzi. Sequence analyses of T. cruzi TR genes revealed their divergency; they do not share such characteristics as sequence similarity or biased cellular location predicted by the presence of a signal sequence or transmembrane domain(s). In contrast, T. cruzi TR proteins seemed to possess significant antigenicity. A number of previously characterized T. cruzi antigens were detected by this computational screening, and several of those antigens contained a large TR domain. Further analyses of the T. cruzi genome demonstrated that previously uncharacterized TR proteins in this organism may also be immunodominant. Taken together, T. cruzi is rich in large TR domain-containing proteins with immunological significance; it is worthwhile further analyzing such characteristics of TR proteins as the copy number and consensus sequence of the repeats to determine whether they might contribute to the biological variability of T. cruzi strains with regard to induced immunological responses, host susceptibility, disease outcomes, and pathogenicity.

Synthesis of MUC1 Peptide and Glycopeptide Dendrimers

2009 ◽  
Vol 62 (10) ◽  
pp. 1339 ◽  
Author(s):  
Candy K. Y. Chun ◽  
Richard J. Payne
Keyword(s):  
Peptide Synthesis ◽  
Tandem Repeat ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Cycloaddition Reaction ◽  
Repeat Sequence ◽  
Tandem Repeat Sequence ◽  
Multiple Copies ◽  
Fmoc Strategy

Several dendrimers possessing multiple copies of peptides and glycopeptides belonging to the MUC1 eicosapeptide tandem repeat sequence have been prepared. Fmoc-strategy solid-phase peptide synthesis was used to construct the peptides and glycopeptides, which were conjugated to suitably functionalized dendrimer cores using the copper-catalyzed azide-alkyne cycloaddition reaction to produce multivalent peptide and glycopeptide dendrimers.

scholarly journals New Tools for Hop Cytogenomics: Identification of Tandem Repeat Families from Long-Read Sequences of Humulus lupulus

2020 ◽  
Author(s):  
Katherine A. Easterling ◽  
Nicholi J. Pitra ◽  
Taylan B. Morcol ◽  
Jenna R. Aquino ◽  
Lauren G. Lopes ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Mendelian Inheritance ◽  
Repeat Sequence ◽  
Humulus Lupulus ◽  
Comparative Genomic ◽  
Dioecious Species ◽  
Tandem Repeat Sequence ◽  
Genome Maps ◽  
Meiotic Chromosomes ◽  
Long Read

ABSTRACTHop (Humulus lupulus L.) is known for its use as a bittering agent in beer and has a rich history of cultivation, beginning in Europe and now spanning the globe. There are five wild varieties worldwide, which may have been introgressed with cultivated varieties. As a dioecious species, its obligate outcrossing, non-Mendelian inheritance, and genomic structural variability have confounded directed breeding efforts. Consequently, understanding genome evolution in Humulus represents a considerable challenge, requiring additional resources, including integrated genome maps. In order to facilitate cytogenetic investigations into the transmission genetics of hop, we report here the identification and characterization of 17 new and distinct tandem repeat sequence families. A tandem repeat discovery pipeline was developed using k-mer filtering and dot plot analysis of PacBio long-read sequences from the hop cultivar Apollo. We produced oligonucleotide FISH probes from conserved regions of HuluTR120 and HulTR225 and demonstrated their utility to stain meiotic chromosomes from wild hop, var. neomexicanus. The HuluTR225 FISH probe hybridized to several loci per nucleus and exhibited irregular, non-Mendelian transmission in male meiocytes of wild hop. Collectively, these tandem repeat sequence families not only represent unique and valuable new cytogenetic reagents but also have the capacity to inform genome assembly efforts and support comparative genomic analyses.

Hydrolyzed Casein Influences Intestinal Mucin Gene Expression in the Rat

2008 ◽  
Vol 56 (14) ◽  
pp. 5572-5576 ◽  
Author(s):  
Kyoung-Sik Han ◽  
Amelie Deglaire ◽  
Ranjita Sengupta ◽  
Paul J. Moughan
Keyword(s):  
Gene Expression ◽  
Mucin Gene ◽  
Intestinal Mucin
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