scholarly journals Ferritin does not accumulate iron oxidized by caeruloplasmin

1995 ◽  
Vol 305 (1) ◽  
pp. 21-23 ◽  
Author(s):  
A Treffry ◽  
D Gelvan ◽  
A M Konijn ◽  
P M Harrison
Keyword(s):  
Dissolved Oxygen ◽  
Storage Protein ◽  
Iron Oxidation ◽  
Primary Effect ◽  
Significant Extent ◽  
Iron Storage ◽  
Horse Spleen Ferritin ◽  
Iron Storage Protein

Ferritin is an iron-storage protein ubiquitous in mammals, plants and bacteria. It can be reconstituted, in vitro, from the apoprotein and Fe(II) salts in the presence of dissolved oxygen. Recently it has been reported that caeruloplasmin can facilitate apoferritin reconstitution and that iron oxidized by caeruloplasmin is sequestered within the ferritin shell. Here we show that the primary effect of adding caeruloplasmin to horse spleen ferritin during reconstitution is the competition between the two molecules for the iron. This competition results in overall increased rates of iron oxidation and a mixture of products, namely iron-containing ferritin and iron hydroxy polymers attached to caeruloplasmin. Iron oxidized by caeruloplasmin is not incorporated, to any significant extent, into horse spleen ferritin.

scholarly journals Ferroxidase activity of ferritin: effects of pH, buffer and Fe(II) and Fe(III) concentrations on Fe(II) autoxidation and ferroxidation

1999 ◽  
Vol 338 (3) ◽  
pp. 615-618 ◽  
Author(s):  
Xiaoke YANG ◽  
N. Dennis CHASTEEN
Keyword(s):  
Ph Control ◽  
Alternative Mechanism ◽  
Experimental Conditions ◽  
Iron Storage ◽  
Ferroxidase Activity ◽  
Effects Of Ph ◽  
Ph Stat ◽  
Ph Buffer ◽  
Horse Spleen Ferritin

It is widely accepted that iron deposition in the iron storage protein ferritin in vitro involves Fe(II) oxidation, and that ferritin facilitates this oxidation at a ferroxidase site on the protein. However, these views have recently been questioned, with the protein ferroxidase activity instead being attributed to autoxidation from the buffer alone. Ligand exchange between another protein with ferroxidase activity and ferritin has been proposed as an alternative mechanism for iron incorporation into ferritin. In the present work, a pH stat apparatus is used to eliminate the influence of buffers on iron(II) oxidation. Here we show that the recent experiments questioning the ferroxidase activity of ferritin were flawed by inadequate pH control, that buffers actually retard rather than facilitate iron(II) oxidation, and that horse spleen ferritin has ferroxidase activity when measured under proper experimental conditions. Furthermore, high pH (7.0), a high Fe(II) concentration and the presence of Fe(III) all favour Fe(II) autoxidation in the presence or absence of ferritin.

Analysis of Iron in Ferritin, the Iron-Storage Protein: A General Chemistry Experiment

1998 ◽  
Vol 75 (4) ◽  
pp. 437 ◽  
Author(s):  
Maureen J. Donlin ◽  
Regina F. Frey ◽  
Christopher Putnam ◽  
Jody Proctor ◽  
James K. Bashkin
Keyword(s):  
General Chemistry ◽  
Storage Protein ◽  
Protein A ◽  
Iron Storage ◽  
Chemistry Experiment ◽  
Iron Storage Protein

scholarly journals Preparation of platinum nanoparticles using iron(ii) as reductant and photosensitized H2 generation on an iron storage protein scaffold

RSC Advances ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 5551-5559
Author(s):  
Brenda S. Benavides ◽  
Silvano Valandro ◽  
Donald M. Kurtz
Keyword(s):  
Heavy Chain ◽  
Platinum Nanoparticles ◽  
Storage Protein ◽  
Iron Storage ◽  
Protein Scaffold ◽  
H2 Generation ◽  
Iron Storage Protein

An assembly of platinum nanoparticles produced by Fe(ii) reduction of Pt(ii) and stabilized by human heavy chain ferritin's native catalysis of Fe(ii)(aq) autoxidation functions as an efficient photosensitized H2 evolution catalyst.

Ferritin as an iron-storage protein: mechanisms of iron uptake

1986 ◽  
Vol 27 (4) ◽  
pp. 287-293 ◽  
Author(s):  
Pauline M. Harrison ◽  
Amyra Treffry ◽  
Terence H. Lilley
Keyword(s):  
Storage Protein ◽  
Iron Uptake ◽  
Iron Storage ◽  
Iron Storage Protein

Increased Expression of Ferritin, an Iron-storage Protein, in Specific Regions of the Parahippocampal Cortex of Epileptic Rats

Epilepsia ◽  
2005 ◽  
Vol 46 (9) ◽  
pp. 1371-1379 ◽  
Author(s):  
Jan A. Gorter ◽  
Ana R.M. Mesquita ◽  
Erwin A. van Vliet ◽  
Fernando H. Lopes da Silva ◽  
Eleonora Aronica
Keyword(s):  
Storage Protein ◽  
Iron Storage ◽  
Parahippocampal Cortex ◽  
Iron Storage Protein

Intracellular Sequestration of223Ra by the Iron-Storage Protein Ferritin

2005 ◽  
Vol 164 (2) ◽  
pp. 230-233 ◽  
Author(s):  
Michael J. Atkinson ◽  
Marianne T. Spanner ◽  
Michael Rosemann ◽  
Utz Linzner ◽  
Walter A. Müller ◽  
...  
Keyword(s):  
Storage Protein ◽  
Iron Storage ◽  
Intracellular Sequestration ◽  
Iron Storage Protein
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