scholarly journals Isolation and characterization of CD47 glycoprotein: a multispanning membrane protein which is the same as integrin-associated protein (IAP) and the ovarian tumour marker OA3

1994 ◽  
Vol 304 (2) ◽  
pp. 525-530 ◽  
Author(s):  
W J Mawby ◽  
C H Holmes ◽  
D J Anstee ◽  
F A Spring ◽  
M J A Tanner
Keyword(s):  
Membrane Protein ◽  
Tumour Marker ◽  
Ovarian Tumour ◽  
Immunocytochemical Staining ◽  
Intact Protein ◽  
Isolation And Characterization ◽  
Glycosylation Sites ◽  
The Brain

The CD47 glycoprotein was isolated from human erythrocytes by immunoprecipitation using monoclonal antibody (mAb) BRIC-125. Enzymic deglycosylation of the protein showed it contained N-linked oligosaccharides, and trypsin proteolysis of the protein in situ in the erythrocyte membrane cleaved it into two portions, one of which was glycosylated. Both the intact protein and the glycosylated fragment had blocked N-termini. Amino acid sequence was obtained from several proteolytic fragments of CD47. Comparison with the sequence database showed the protein to be very similar to or identical with OA3, a multispanning membrane protein. The protein also appears to be the same as the integrin-associated protein, which has a role in cell adhesion in non-erythroid cells. CD47 has six potential N-glycosylation sites, five of which are in an Ig superfamily domain. We show that three of these sites carry N-glycans in erythrocytes. Immunocytochemical staining of human tissues showed that CD47 was broadly distributed on mesenchyme and epithelia at multiple sites. Reactivity was particularly prominent in surface and ductular epithelia, and in the brain. The possible roles of the CD47 glycoprotein are discussed.

Isolation and Characterization of a Novel Bioactive Peptide, Carassius RFamide (C-RFa), from the Brain of the Japanese Crucian Carp

1998 ◽  
Vol 242 (2) ◽  
pp. 436-440 ◽  
Author(s):  
Masaaki Fujimoto ◽  
Ken-ichi Takeshita ◽  
Xiaoyan Wang ◽  
Ikuo Takabatake ◽  
Yuko Fujisawa ◽  
...  
Keyword(s):  
Crucian Carp ◽  
Bioactive Peptide ◽  
Isolation And Characterization ◽  
The Brain

scholarly journals Characterization of a Vesivirus Associated with an Outbreak of Acute Hemorrhagic Gastroenteritis in Domestic Dogs

2018 ◽  
Vol 56 (5) ◽  
Author(s):  
Randall W. Renshaw ◽  
Jennifer Griffing ◽  
Jaime Weisman ◽  
Lisa M. Crofton ◽  
Melissa A. Laverack ◽  
...  
Keyword(s):  
Intestinal Tract ◽  
Virus Isolation ◽  
Cho Cell ◽  
Mild Disease ◽  
Reverse Transcription Pcr ◽  
The Brain ◽  
Housing Facility ◽  
Diagnostic Investigations

ABSTRACT Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence homology to a vesivirus within the group colloquially known as the vesivirus 2117 strains that were originally identified as contaminants in CHO cell bioreactors. In situ hybridization and reverse transcription-PCR assays of tissues from the four deceased dogs confirmed the presence of canine vesivirus (CaVV) nucleic acids that localized to endothelial cells of arterial and capillary blood vessels. CaVV nucleic acid corresponded to areas of necrosis and hemorrhage primarily in the intestinal tract, but also in the brain of one dog with nonsuppurative meningoencephalitis. This is the first report of an atypical disease association with a putative hypervirulent vesivirus strain in dogs, as all other known strains of CaVV appear to cause nonclinical infections or relatively mild disease. After identification of the CU-296 vesivirus strain from this outbreak, four additional CaVV strains were amplified from unrelated fecal specimens and archived stocks provided by other laboratories. Broader questions include the origins, reservoir(s), and potential for reemergence and spread of these related CaVVs.

scholarly journals Identification and Characterization of Four Autophagy-Related Genes That Are Expressed in Response to Hypoxia in the Brain of the Oriental River Prawn (Macrobrachium nipponense)

2019 ◽  
Vol 20 (8) ◽  
pp. 1856 ◽  
Author(s):  
Shengming Sun ◽  
Ying Wu ◽  
Hongtuo Fu ◽  
Xianping Ge ◽  
Hongzheng You ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Open Reading Frames ◽  
Dependent Manner ◽  
Macrobrachium Nipponense ◽  
Oriental River Prawn ◽  
Adverse Environmental Conditions ◽  
The Brain ◽  
Identification And Characterization

Autophagy is a cytoprotective mechanism triggered in response to adverse environmental conditions. Herein, we investigated the autophagy process in the oriental river prawn (Macrobrachium nipponense) following hypoxia. Full-length cDNAs encoding autophagy-related genes (ATGs) ATG3, ATG4B, ATG5, and ATG9A were cloned, and transcription following hypoxia was explored in different tissues and developmental stages. The ATG3, ATG4B, ATG5, and ATG9A cDNAs include open reading frames encoding proteins of 319, 264, 268, and 828 amino acids, respectively. The four M. nipponense proteins clustered separately from vertebrate homologs in phylogenetic analysis. All four mRNAs were expressed in various tissues, with highest levels in brain and hepatopancreas. Hypoxia up-regulated all four mRNAs in a time-dependent manner. Thus, these genes may contribute to autophagy-based responses against hypoxia in M. nipponense. Biochemical analysis revealed that hypoxia stimulated anaerobic metabolism in the brain tissue. Furthermore, in situ hybridization experiments revealed that ATG4B was mainly expressed in the secretory and astrocyte cells of the brain. Silencing of ATG4B down-regulated ATG8 and decreased cell viability in juvenile prawn brains following hypoxia. Thus, autophagy is an adaptive response protecting against hypoxia in M. nipponense and possibly other crustaceans. Recombinant MnATG4B could interact with recombinant MnATG8, but the GST protein could not bind to MnATG8. These findings provide us with a better understanding of the fundamental mechanisms of autophagy in prawns.

scholarly journals High-throughput Isolation and Characterization of Untagged Membrane Protein Complexes: Outer Membrane Complexes ofDesulfovibrio vulgaris

2012 ◽  
Vol 11 (12) ◽  
pp. 5720-5735 ◽  
Author(s):  
Peter J. Walian ◽  
Simon Allen ◽  
Maxim Shatsky ◽  
Lucy Zeng ◽  
Evelin D. Szakal ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
High Throughput ◽  
Protein Complexes ◽  
Isolation And Characterization ◽  
Membrane Protein Complexes

scholarly journals In situ characterization of the brain–microdevice interface using Device Capture Histology

2011 ◽  
Vol 201 (1) ◽  
pp. 67-77 ◽  
Author(s):  
Andrew J. Woolley ◽  
Himanshi A. Desai ◽  
Mitchell A. Steckbeck ◽  
Neil K. Patel ◽  
Kevin J. Otto
Keyword(s):  
In Situ Characterization ◽  
The Brain

Isolation and characterization of a zebra finch aromatase cDNA: in situ hybridization reveals high aromatase expression in brain

1994 ◽  
Vol 24 (1-4) ◽  
pp. 227-237 ◽  
Author(s):  
P. Shen ◽  
C.W. Campagnoni ◽  
K. Kampf ◽  
B.A. Schlinger ◽  
A.P. Arnold ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Zebra Finch ◽  
Aromatase Expression ◽  
Isolation And Characterization
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