scholarly journals Studies on cytochrome c-heparin interactions by differential scanning calorimetry

1994 ◽  
Vol 297 (1) ◽  
pp. 99-101 ◽  
Author(s):  
J Bágel'ová ◽  
M Antalík ◽  
M Bona
Keyword(s):  
Differential Scanning Calorimetry ◽  
Ionic Strength ◽  
Cytochrome C ◽  
Thermal Denaturation ◽  
High Sensitivity ◽  
Scanning Calorimetry ◽  
Protein Molecules ◽  
Low Ionic Strength ◽  
Thermotropic Properties ◽  
Important Shift

The effects of heparin on the thermotropic properties of ferricytochrome c have been studied using high-sensitivity differential scanning calorimetry. Saturating concentrations of heparin at low ionic strength induced an important shift of the transition temperature Tm from 84.1 degrees C to 59.8 degrees C. This was accompanied by unusually large cooperativity of thermal denaturation of this complex, indicating strong intermolecular interactions between protein molecules. The destabilization of cytochrome c when mixed with heparin was not observed at high ionic strength, under which conditions complex was not formed.

scholarly journals Interaction of plant alkaloid, berberine, with zwitterionic and negatively charged phospholipid bilayers

2011 ◽  
Vol 34 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Justyna Gąsiorowska ◽  
Olga Wesołowska ◽  
Krystyna Michalak
Keyword(s):  
Phase Transition ◽  
Differential Scanning Calorimetry ◽  
Fluorescence Quenching ◽  
Electrostatic Interactions ◽  
Phospholipid Bilayers ◽  
Scanning Calorimetry ◽  
Double Peak ◽  
Pharmacological Activities ◽  
First Time ◽  
Thermotropic Properties

Interaction of plant alkaloid, berberine, with zwitterionic and negatively charged phospholipid bilayers Berberine exhibits many pharmacological activities e.g. antibacterial, anti-inflammatory, antiproliferative and apoptosis-inducing. Interaction of berberine with model membranes was studied for the first time using differential scanning calorimetry, fluorescence spectroscopy and turbidity measurements. Influence of berberine on thermotropic properties of bilayers formed from zwitterionic DMPC was insignificant, whereas in bilayers formed from negatively charged DMPG berberine reduced the temperature and cooperativity of main phospholipid phase transition. In higher concentrations berberine induced complex double-peak transition, with the new peak appearing in temperature higher than the original one. It suggested the interaction of the alkaloid with lipid headgroup region of the bilayer. Additionally, berberine quenched fluorescence of Prodan to a higher extent than Laurdan that pointed to stronger interaction with membrane segments close to its surface. Berberine-induced fluorescence quenching of both probes was more pronounced in DPPG than in DPPC bilayers. It was concluded that electrostatic interactions governed berberine association with model membrane.

Thermodynamics of Conformational Ordering of ι-Carrageenan in KCl Solutions Using High-Sensitivity Differential Scanning Calorimetry

2001 ◽  
Vol 2 (3) ◽  
pp. 864-873 ◽  
Author(s):  
Valerij Ya. Grinberg ◽  
Natalia V. Grinberg ◽  
Anatoly I. Usov ◽  
Nadezhda P. Shusharina ◽  
Alexei R. Khokhlov ◽  
...  
Keyword(s):  
Differential Scanning Calorimetry ◽  
High Sensitivity ◽  
Scanning Calorimetry ◽  
Conformational Ordering
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