scholarly journals Use of site-directed mutagenesis of allele-specific PCR primers to identify the GSTM1 A, GSTM1 B, GSTM1 A,B and GSTM1 null polymorphisms at the glutathione S-transferase, GSTM1 locus

1993 ◽  
Vol 295 (1) ◽  
pp. 313-315 ◽  
Author(s):  
A A Fryer ◽  
L Zhao ◽  
J Alldersea ◽  
W R Pearson ◽  
R C Strange
Keyword(s):  
Pcr Primers ◽  
Single Step ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Glutathione S Transferase ◽  
Specific Pcr ◽  
Target Dna ◽  
Allele Specific ◽  
Pcr Method ◽  
Allele Specific Pcr

We describe the identification of the GSTM1 null, GSTM1 A, GSTM1 B and GSTM1 A,B polymorphisms at the glutathione S-transferase GSTM1 locus using a single-step PCR method. Target DNA was amplified using primers to intron 6 and exon 7 with site-directed mutagenesis being used to introduce a restriction site in DNA amplified from GSTM1 *A, thereby allowing differentiation of this allele and GSTM1 *B. The accuracy of this approach in identifying the GSTM1 A, GSTM1 B, GSTM1 A,B and GSTM1 null polymorphisms was confirmed by comparison with, firstly, an established PCR method that distinguishes GSTM1 *0 homozygotes from individuals with the other GSTM1 genotypes and, secondly, GSTM1 phenotypes determined using chromatofocusing.

scholarly journals Identification of Phytophthora alni subspecies in riparian stands in the Czech Republic

2013 ◽  
Vol 49 (Special Issue) ◽  
pp. S3-S10 ◽  
Author(s):  
P. Štěpánková ◽  
K. Černý ◽  
V. Strnadová ◽  
P. Hanáček ◽  
M. Tomšovský
Keyword(s):  
Czech Republic ◽  
Alnus Glutinosa ◽  
Pcr Primers ◽  
The Czech Republic ◽  
Specific Pcr ◽  
Related Taxon ◽  
Allele Specific ◽  
Allele Specific Pcr ◽  
Subspecific Level

In the Czech Republic, Phytophthora alni was first confirmed in 2001 and the pathogen has been quickly spreading and occupying almost the whole area of the country. The pathogen attacks Alnus glutinosa or A. incana to a lesser extent and causes considerable losses of alder trees along hundreds of kilometres of riverbanks. The aim of our work was to perform the identification of P. alni isolates at the subspecific level using PCR and to determine the frequencies and distribution of particular subspecies. The allele-specific PCR primers focused on allele diversity of orthologs of ASF-like, TRP1, RAS-Ypt, and GPA1 genes were selected for identification. Eighty-eight per cent of the 59 analysed isolates belonged to P. alni ssp. alni while 12% were P. alni ssp. uniformis. P. alni ssp. multiformis has not been recorded in the country till now. The two subspecies differed in distribution. P. alni ssp. alni dominated in riparian stands along broader rivers in lowlands and the results confirmed the more effective spreading of P. alni ssp. alni based on its higher aggressiveness and ecological advantage. P. alni ssp. uniformis was acquired rather from riparian stands of small watercourses at higher altitudes. The insular distribution of P. alni ssp. uniformis may represent the remains of its former occurrence. Therefore, P. alni ssp. uniformis may be an indigenous subspecies suppressed by the more aggressive related taxon.

Simultaneous DNA Typing of Human Platelet Antigens 2, 3 and 4 by an Allele-Specific PCR Method

Vox Sanguinis ◽  
1995 ◽  
Vol 68 (4) ◽  
pp. 225-230
Author(s):  
Shigenori Tanaka ◽  
Atsuko Taniue ◽  
Nobuo Nagao ◽  
Shiro Ohnoki ◽  
Hirotoshi Shibata ◽  
...  
Keyword(s):  
Human Platelet ◽  
Dna Typing ◽  
Specific Pcr ◽  
Allele Specific ◽  
Pcr Method ◽  
Allele Specific Pcr

Rapid single-step FCGR3A genotyping based on SYBR Green I fluorescence in real-time multiplex allele-specific PCR

2003 ◽  
Vol 277 (1-2) ◽  
pp. 185-192 ◽  
Author(s):  
Sébastien Dall'Ozzo ◽  
Christian Andres ◽  
Pierre Bardos ◽  
Hervé Watier ◽  
Gilles Thibault
Keyword(s):  
Real Time ◽  
Single Step ◽  
Sybr Green ◽  
Sybr Green I ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

Simultaneous DNA Typing of Human Platelet Antigens 2, 3 and 4 by an Allele-Specific PCR Method

Vox Sanguinis ◽  
1995 ◽  
Vol 68 (4) ◽  
pp. 225-230 ◽  
Author(s):  
Shigenori Tanaka ◽  
Atsuko Taniue ◽  
Nobuo Nagao ◽  
Shiro Ohnoki ◽  
Hirotoshi Shibata ◽  
...  
Keyword(s):  
Human Platelet ◽  
Dna Typing ◽  
Specific Pcr ◽  
Allele Specific ◽  
Pcr Method ◽  
Allele Specific Pcr
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