scholarly journals Characterization of a new oriental-mustard (Brassica juncea) allergen, Braj IE: detection of an allergenic epitope

1993 ◽  
Vol 293 (3) ◽  
pp. 625-632 ◽  
Author(s):  
R I Monsalve ◽  
M A Gonzalez de la Peña ◽  
L Menendez-Arias ◽  
C Lopez-Otin ◽  
M Villalba ◽  
...  
Keyword(s):  
Amino Acids ◽  
Monoclonal Antibody ◽  
Brassica Juncea ◽  
Antigenic Determinant ◽  
Major Allergen ◽  
Sinapis Alba ◽  
Histidine Residue ◽  
Yellow Mustard ◽  
Oriental Mustard

Bra j IE, a major allergen from oriental-mustard (Brassica juncea) seeds, has been isolated and characterized. Its primary structure has been elucidated. This protein is composed of two chains (37 and 92 amino acids) linked by disulphide bridges. The amino acid sequence obtained is closely related to that previously determined for Sin a I, an allergen isolated from yellow mustard (Sinapis alba). A common epitope has been detected in the large chain of both Bra j IE and Sin a I by means of electroblotting and immunodetection with 2B3, which is a monoclonal antibody raised against the yellow-mustard allergen. A histidine residue of the large chain of both mustard allergens has been found to be essential for the recognition by 2B3 antibody. A synthetic multiantigenic peptide containing this His was recognized by 2B3 as well as by sera of mustard-hypersensitive individuals. Therefore this antigenic determinant must be involved in the allergenicity of these proteins.

Midge (Diptera: Cecidomyiidae) injury to Brassicaceae in field trials in northeastern Saskatchewan, Canada

2018 ◽  
Vol 150 (5) ◽  
pp. 637-651 ◽  
Author(s):  
Lars Andreassen ◽  
Juliana Soroka ◽  
Larry Grenkow ◽  
Owen Olfert ◽  
Rebecca H. Hallett
Keyword(s):  
Brassica Napus ◽  
Field Trials ◽  
Sinapis Alba ◽  
Camelina Sativa ◽  
Undescribed Species ◽  
Yellow Mustard ◽  
Field Crops ◽  
Ethiopian Mustard ◽  
Contarinia Nasturtii ◽  
Oriental Mustard

AbstractTo determine resistance of Brassicaceae field crops to Contarinia Róndani (Diptera: Cecidomyiidae) midge complex (Contarinia nasturtii Kieffer and Contarinia undescribed species), field trials of two different host assemblages were undertaken near Melfort, Saskatchewan, Canada in 2014 and repeated in 2015. In both years the first midge adults appeared in early July, when most plants were starting to flower, and a second generation occurred in mid-August, past the period of crop susceptibility. In a trial studying 18 lines of six brassicaceous species, the lowest probability of midge injury was found on Camelina sativa (Linnaeus) Crantz lines in both years. No differences were found in the probability of midge injury among any of the 13 Brassica Linnaeus species lines tested, including commercial glyphosate-resistant and glufosinate-resistant Brassica napus Linnaeus canola lines, Ethiopian mustard (Brassica carinata Braun), brown or oriental mustard (Brassica juncea (Linnaeus) Czernajew), or Polish canola (Brassica rapa Linnaeus) lines. Probability of midge injury on Sinapis alba Linnaeus yellow mustard lines reached levels between those on Camelina sativa lines and those on Brassica lines. A second trial examining 14 current commercial glyphosate-resistant Brassica napus canola cultivars found no differences in susceptibility to midge feeding among any cultivars tested. More plants were damaged in 2015 in both studies, and damage reached maximum levels earlier in 2015 than in 2014.

Species specificity of a monoclonal antibody produced to Naegleria fowleri and partial characterization of its antigenic determinant

2000 ◽  
Vol 86 (8) ◽  
pp. 634-641 ◽  
Author(s):  
Fabienne L. Réveiller ◽  
Francine Marciano-Cabral ◽  
Pierre Pernin ◽  
Pierre-André Cabanes ◽  
Stéphane Legastelois
Keyword(s):  
Monoclonal Antibody ◽  
Antigenic Determinant ◽  
Species Specificity ◽  
Partial Characterization ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri

Productivity and quality of canola and mustard cultivars under weed competition

2008 ◽  
Vol 88 (2) ◽  
pp. 367-372 ◽  
Author(s):  
H. J. Beckie ◽  
E. N. Johnson ◽  
R. E. Blackshaw ◽  
Y. Gan
Keyword(s):  
Brassica Juncea ◽  
Seed Yield ◽  
Weed Management ◽  
Sinapis Alba ◽  
Integrated Weed Management ◽  
Weed Competition ◽  
Weed Interference ◽  
Canola Quality ◽  
Oriental Mustard

Competitive crops or cultivars can be an important component of integrated weed management systems. A study was conducted from 2003 to 2006 at four sites across semiarid prairie ecoregions in Saskatchewan and Alberta to investigate the productivity and quality of canola (Brassica napusL.) and mustard cultivars under weed competition. Four open-pollinated canola cultivars, four hybrid canola cultivars, two canola-quality mustard and two oriental mustard cultivars [Brassica juncea (L.) Czern. & Coss.], and two yellow mustard (Sinapis alba L.) cultivars were grown under weedy and weed-free conditions. When combined across site-years, crop aboveground biomass at maturity and seed yield were reduced by weed interference, except for yellow and oriental mustard. However, seed oil and protein content of cultivars were not affected by weed competition. Among crop types, yellow and oriental mustard were best able to maintain biomass and seed yield under weed interference, followed in decreasing order of competitiveness by hybrid and open-pollinated canola, then canola-quality mustard. Key words: Brassica napus, Brassica juncea, Sinapis alba, weed competition

FAB CID-MS/MS characterization of tetrasaccharide tri- and tetrasulfate derived from the antigenic determinant recognized by the anti-chondroitin sulfate monoclonal antibody MO-225

1995 ◽  
Vol 12 (3) ◽  
pp. 282-289 ◽  
Author(s):  
Tadashi II ◽  
Masayuki Kubota ◽  
Takashi Hirano ◽  
Mamoru Ohashi ◽  
Keiichi Yoshida ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Chondroitin Sulfate ◽  
Antigenic Determinant

Response of mustard to fertilization, seeding date, and seeding rate in southern Alberta

2006 ◽  
Vol 86 (2) ◽  
pp. 353-362 ◽  
Author(s):  
R. H. McKenzie ◽  
A. B. Middleton ◽  
E. Bremer
Keyword(s):  
Brassica Juncea ◽  
Seed Yield ◽  
Yield Potential ◽  
Seeding Rate ◽  
Fertilizer Rate ◽  
Yellow Mustard ◽  
Seeding Date ◽  
Southern Alberta ◽  
P Fertilizer ◽  
Oriental Mustard

Yellow mustard (Sinapsis alba L.), brown mustard (Brassica juncea L.), and oriental mustard (B. juncea) have been grown in Alberta since the 1950s, but limited agronomic information specific for this crop is available. The objective of this study was to determine the response of mustard to fertilization, seeding date and seeding rate in southern Alberta. Field experiments were conducted at 20 field sites over a 4-yr period (1999–2002) under irrigated and dryland (fallow and stubble) conditions. Five experiments were conducted with the following treatments: (1) N fertilizer rate (0, 30, 60, 90 and 120 kg N ha-1), (2) urea placement (seed-placed and side-banded urea at rates of 0 to 120 kg N ha-1), (3) P fertilizer rate (0, 6.5, and 13.1 kg P ha-1), (4) S fertilizer rate (0, 10 and 20 kg S ha-1), and (5) seeding date (three dates at approximately 10-d intervals) and seeding rate (target plant densities of 75, 125, 175, 225, and 300 plants m-2). Experiment 1 was conducted with yellow mustard (AC Pennant), oriental mustard (Forge), brown mustard (Commercial Brown) and canola (Q2) (Brassica napus L.), while the remaining experiments were only conducted with yellow mustard. For maximum seed yield, mustard required 95 kg of available N Mg-1 of potential seed yield. Potential yields were closely related to available moisture, increasing 7 to 8 kg ha-1 for every mm increase in available moisture above a minimum moisture requirement of 90 mm. Seed-placed urea reduced plant stand at rates as low as 30 kg N ha-1 and reduced seed yield at rates of 60 to 120 kg N ha-1. Fourteen of 20 sites had a greater than 3% positive yield benefit due to P fertilizer. Mustard did not benefit from S fertilizer application. Delay in seeding by 3–4 wk, compared with seeding in late April to mid-May, reduced seed yield of yellow mustard by an average of 37%. Seed yield increased with seeding rate, but the maximum gain in seed yield due to high seeding rates was only 200 kg ha-1, with significant increases obtained only under very dry conditions. Early seeding and adequate N fertility were the most important agronomic practices for achieving high yields of mustard in southern Alberta. Key words: Sinapsis alba, Brassica juncea, yield, oil, nitrogen, phosphorus, sulfur, water-use efficiency

scholarly journals Primary structure of the major allergen of yellow mustard (Sinapis alba L.) seed, Sin a I

2005 ◽  
Vol 177 (1) ◽  
pp. 163-166
Author(s):  
Luis MENÉNDEZ-ARIAS ◽  
Ignacio MONEO ◽  
Javier DOMÍNGUEZ ◽  
Rosalía RODRÍGUEZ
Keyword(s):  
Primary Structure ◽  
Major Allergen ◽  
Sinapis Alba ◽  
Yellow Mustard ◽  
Sinapis Alba L

scholarly journals A monoclonal antibody to vasopressin: preparation, characterization, and application in immunocytochemistry.

1982 ◽  
Vol 30 (12) ◽  
pp. 1249-1260 ◽  
Author(s):  
A Hou-Yu ◽  
P H Ehrlich ◽  
G Valiquette ◽  
D L Engelhardt ◽  
W H Sawyer ◽  
...  
Keyword(s):  
Amino Acids ◽  
Monoclonal Antibody ◽  
Aromatic Amino Acids ◽  
Arginine Vasotocin ◽  
Antigenic Determinants ◽  
Tissue Fixation ◽  
Extensive Investigation ◽  
Carrier Proteins ◽  
The Subject

The hypothalamo-neurohypophysial system, containing the hormones oxytocin (OT) and vasopressin (VP) and their associated carrier proteins, the neurophysins (NPS), has been the subject of extensive investigation for more than 40 years. This system has been reinvestigated during the last decade by application of immunocytochemical methods employing the rabbit antisera to the hormones and NPS. In this study we describe the preparation and characterization of a monoclonal antibody to VP and its application in immunohistochemistry. The antibody did not cross-react with OT or arginine vasotocin (AVT). Its antigenic determinants as characterized by absorption with various VP analogs included two aromatic amino acids: Phe in position 3, and to a lesser extent Tyr in 2. Tissue fixation with formaldehyde resulted in inadequate immunostaining as compared to glutaraldehyde, most likely due to interference with the aromatic amino acid determinants by the former fixative.

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