scholarly journals Biochemical properties of amphibian bone morphogenetic protein-4 expressed in CHO cells

1993 ◽  
Vol 291 (2) ◽  
pp. 413-417 ◽  
Author(s):  
A Suzuki ◽  
S Nishimatsu ◽  
A Shoda ◽  
K Takebayashi ◽  
K Murakami ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Cho Cells ◽  
Northern Blot Analysis ◽  
Chinese Hamster ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Biochemical Properties ◽  
Expression Vectors ◽  
Bone Morphogenetic Protein 4 ◽  
Morphogenetic Protein

The biochemical properties of recombinant amphibian bone morphogenetic protein-4 (BMP-4), the cDNA of which has been cloned recently by screening of a Xenopus cDNA library, was characterized. The protein was expressed by the transfection of Chinese hamster ovary (CHO) cells with the cDNA cloned into expression vectors bearing a cytomegalovirus promoter or a simian virus 40 promoter. Northern-blot analysis showed that the latter vector was more efficient for Xenopus BMP-4 expression. Specific antiserum against Xenopus BMP-4 peptide demonstrated that the protein is synthesized as a large precursor, processed to the mature form and then secreted from the cells as a homodimer. Analysis of the biological activity in the conditioned medium revealed that Xenopus BMP-4 has a potent alkaline phosphatase-inducing activity on mouse osteoblastic cells.

scholarly journals Quantitative analysis of intercellular adhesive specificity in freshly explanted and cultured cells.

1981 ◽  
Vol 90 (1) ◽  
pp. 55-62 ◽  
Author(s):  
F Sieber ◽  
S Roseman
Keyword(s):  
Quantitative Analysis ◽  
Cho Cells ◽  
Binomial Distribution ◽  
Cultured Cells ◽  
Chinese Hamster ◽  
Simian Virus 40 ◽  
Cell Types ◽  
Simian Virus ◽  
Fluorescent Label ◽  
3T3 Cells

A new method is presented for the quantitative analysis of intercellular adhesive specificity. In this assay, two cell types are mixed, one unlabeled and the other labeled with the fluorescent dye, fluorescamine [4-phenylspiro(feran-2[3H],1'-phthalan)-3,3'-dione]. The resulting aggregates are analyzed by fluorescence microscopy to determine the number of labeled and unlabeled cells per aggregate. Random (nonspecific) aggregation was characterized by a binomial distribution, and adhesive specificity was accordingly quantified by the deviation (as determined by a chi-square test) from the calculated binomial distribution. The labeling procedure was simple and rapid, and experiments with 18 different cell types showed that it did not affect cell viability, morphology, rate and extent of adhesion, plating efficiency, and the capability of myogenic cells to undergo terminal differentiation. Most important, assays with morphologically identifiable cell pairs indicated that the fluorescent label neither induced apparent nor destroyed existing adhesive specificity. The most pronounced adhesive specificities were observed with freshly explanted cells from adult tissues and also with mixtures of simian virus 40-transformed and nontransformed BALB/c 3T3 cells. A glucosamine-6-phosphate N-acetylase-deficient mutant 3T3 line (AD6), however, aggregated randomly with parental 3T3 cells. Lectin-resistant mutant Chinese hamster ovary (CHO) cells displayed marginal adhesive specificity when mixed with normal CHO cells.

scholarly journals Identification of bone morphogenetic protein 4 in the saliva after the placement of fixed orthodontic appliance

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Lovorka Grgurevic ◽  
Ruder Novak ◽  
Grgur Salai ◽  
Vladimir Trkulja ◽  
Lejla Ferhatovic Hamzic ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Morphogenetic Protein ◽  
Bone Remodeling ◽  
Small Sample ◽  
Central Position ◽  
Published Data ◽  
Bone Morphogenetic Protein 4 ◽  
Orthodontic Appliance ◽  
Morphogenetic Protein ◽  
Fixed Orthodontic Appliance

Abstract Background This study was conducted in order to explore the effects of orthodontic tooth movement (OTM) on the changes of salivary proteome. This prospective observational pilot study recruited 12 healthy teenage boys with malocclusion treated with a fixed orthodontic appliance and 6 appropriate control participants. Saliva samples were collected a day before and at 0, 2, 7, and 30 days after initialization of treatment, corresponding to the initial, lag, and post-lag phases of OTM. Pooled samples were analyzed by liquid chromatography-mass spectrometry, ELISA, and Western blotting. To date, there is no published data on the presence of BMP molecules or their antagonists in the saliva or in the gingival cervical fluid related to orthodontic conditions. Results A total of 198 identified saliva proteins were classified based on their functional characteristics. Proteins involved in bone remodeling were observed exclusively 30 days post appliance placement, including bone morphogenetic protein 4 (BMP4), a BMP antagonist BMP-binding endothelial regulator, insulin-like growth factor-binding protein 3, cytoskeleton-associated protein 4, and fibroblast growth factor 5. Based on the analysis of protein interactions, BMP4 was found to have a central position in this OTM-related protein network. Conclusions The placement of a fixed orthodontic appliance induced occurrence of proteins involved in bone remodeling in the saliva at a time corresponding to the post-lag period of OTM. Limitations of this study include a relatively small sample size, limited time of monitoring patients, and the lack of interindividual variability assessment.

Expression of mRNA of murine bone-related proteins in ectopic bone induced by murine bone morphogenetic protein-4

1994 ◽  
Vol 277 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Seiichi Hirota ◽  
Kunio Takaoka ◽  
Jun Hashimoto ◽  
Takanobu Nakase ◽  
Teiji Takemura ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 4 ◽  
Morphogenetic Protein ◽  
Ectopic Bone ◽  
Related Proteins
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