scholarly journals Characterization of the cytotoxic effect of extracellular ATP in J774 mouse macrophages

1992 ◽  
Vol 288 (3) ◽  
pp. 897-901 ◽  
Author(s):  
M Murgia ◽  
P Pizzo ◽  
T H Steinberg ◽  
F Di Virgilio
Keyword(s):  
Plasma Membrane ◽  
Membrane Permeability ◽  
Cell Types ◽  
Mouse Cell ◽  
Extracellular Atp ◽  
Cell Swelling ◽  
Specific Cell ◽  
Plasma Membrane Permeability ◽  
Experimental Conditions ◽  
Surface Receptors

Extracellular ATP (ATPo) is known to be cytotoxic to many cell types through a mechanism which is largely unknown. Very recently this nucleotide has been shown to cause cell death by apoptosis, probably by interacting with specific cell-surface receptors. In the present study we have investigated the mechanism of ATPo-dependent cytotoxicity in the macrophage-like mouse cell line J774. It has been previously reported that in this cell type ATPo activates trans-membrane Ca2+ and Na+ fluxes and a drastic increase in the plasma-membrane permeability to hydrophilic solutes smaller than 900 Da. These changes are followed by cell swelling and lysis. We show in the present study that, although this nucleotide triggers a rise in the cytoplasmic Ca2+ concentration, neither cell swelling nor lysis is Ca(2+)-dependent. Furthermore, cell lysis is not dependent on Na+ influx, as it is not prevented by iso-osmotic replacement of extracellular Na+ with choline or N-methylglucamine. On the contrary, ATPo-dependent cytotoxicity, but not the ATPo-dependent increase in plasma-membrane permeability, is completely abrogated in sucrose medium. Under our experimental conditions ATPo does not cause DNA fragmentation in J774 cells. We conclude from these findings that ATPo does not cause apoptosis of J774 macrophages and promotes a Ca(2+)- and Na(+)-independent colloido-osmotic lysis.

Extracellular ATP Causes Changes in Plasma Membrane Permeability of Mouse Lymphocytes

1990 ◽  
Vol 603 (1 Biological Ac) ◽  
pp. 427-428
Author(s):  
FRANCESCO VIRGILIO ◽  
ENZO PICELLO ◽  
VINCENZO BRONTE ◽  
PAOLA ZANOVELLO ◽  
DINO COLLAVO
Keyword(s):  
Plasma Membrane ◽  
Membrane Permeability ◽  
Extracellular Atp ◽  
Plasma Membrane Permeability ◽  
Mouse Lymphocytes

Nanotheranostic agents for neurodegenerative diseases

2020 ◽  
Vol 4 (6) ◽  
pp. 645-675
Author(s):  
Parasuraman Padmanabhan ◽  
Mathangi Palanivel ◽  
Ajay Kumar ◽  
Domokos Máthé ◽  
George K. Radda ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Neurodegenerative Diseases ◽  
Cell Types ◽  
Specific Cell ◽  
Ageing Population ◽  
Target Tissue ◽  
Therapeutic Approaches ◽  
Surface Receptors ◽  
Theranostic Agents ◽  
Preclinical Animal Models

Neurodegenerative diseases (NDDs), including Alzheimer's disease (AD) and Parkinson's disease (PD), affect the ageing population worldwide and while severely impairing the quality of life of millions, they also cause a massive economic burden to countries with progressively ageing populations. Parallel with the search for biomarkers for early detection and prediction, the pursuit for therapeutic approaches has become growingly intensive in recent years. Various prospective therapeutic approaches have been explored with an emphasis on early prevention and protection, including, but not limited to, gene therapy, stem cell therapy, immunotherapy and radiotherapy. Many pharmacological interventions have proved to be promising novel avenues, but successful applications are often hampered by the poor delivery of the therapeutics across the blood-brain-barrier (BBB). To overcome this challenge, nanoparticle (NP)-mediated drug delivery has been considered as a promising option, as NP-based drug delivery systems can be functionalized to target specific cell surface receptors and to achieve controlled and long-term release of therapeutics to the target tissue. The usefulness of NPs for loading and delivering of drugs has been extensively studied in the context of NDDs, and their biological efficacy has been demonstrated in numerous preclinical animal models. Efforts have also been made towards the development of NPs which can be used for targeting the BBB and various cell types in the brain. The main focus of this review is to briefly discuss the advantages of functionalized NPs as promising theranostic agents for the diagnosis and therapy of NDDs. We also summarize the results of diverse studies that specifically investigated the usage of different NPs for the treatment of NDDs, with a specific emphasis on AD and PD, and the associated pathophysiological changes. Finally, we offer perspectives on the existing challenges of using NPs as theranostic agents and possible futuristic approaches to improve them.

Synthetic marine guanidines are effective antileishmanial compounds by altering the plasma membrane permeability

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
AG Tempone ◽  
LF Martins ◽  
EG Pinto ◽  
JT Mesquita ◽  
EL Bennett ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Permeability ◽  
Plasma Membrane Permeability

scholarly journals A practical solution to pseudoreplication bias in single-cell studies

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kip D. Zimmerman ◽  
Mark A. Espeland ◽  
Carl D. Langefeld
Keyword(s):  
Single Cell ◽  
Mixed Models ◽  
Random Effect ◽  
Cell Types ◽  
Error Rates ◽  
Specific Cell ◽  
Experimental Conditions ◽  
Type 1 Error ◽  
Sample Correlation ◽  
Inflated Type

AbstractCells from the same individual share common genetic and environmental backgrounds and are not statistically independent; therefore, they are subsamples or pseudoreplicates. Thus, single-cell data have a hierarchical structure that many current single-cell methods do not address, leading to biased inference, highly inflated type 1 error rates, and reduced robustness and reproducibility. This includes methods that use a batch effect correction for individual as a means of accounting for within-sample correlation. Here, we document this dependence across a range of cell types and show that pseudo-bulk aggregation methods are conservative and underpowered relative to mixed models. To compute differential expression within a specific cell type across treatment groups, we propose applying generalized linear mixed models with a random effect for individual, to properly account for both zero inflation and the correlation structure among measures from cells within an individual. Finally, we provide power estimates across a range of experimental conditions to assist researchers in designing appropriately powered studies.

Influence of Low-Energy Ion Irradiation on Plasma Membrane Permeability of Cells

2000 ◽  
Vol 17 (1) ◽  
pp. 61-63
Author(s):  
Dong-Mei Zhang ◽  
Fu-Zhai Cui ◽  
Su-Qin Sun ◽  
You-Bo Lin ◽  
Min-Bo Tian ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Permeability ◽  
Ion Irradiation ◽  
Low Energy ◽  
Plasma Membrane Permeability

A new look at the antibiotic amphotericin B effect on Candida albicans plasma membrane permeability and cell viability functions

2015 ◽  
Vol 44 (1-2) ◽  
pp. 77-90 ◽  
Author(s):  
Barbara Chudzik ◽  
Mateusz Koselski ◽  
Aleksandra Czuryło ◽  
Kazimierz Trębacz ◽  
Mariusz Gagoś
Keyword(s):  
Candida Albicans ◽  
Plasma Membrane ◽  
Cell Viability ◽  
Membrane Permeability ◽  
Amphotericin B ◽  
Plasma Membrane Permeability ◽  
New Look
Sign in / Sign up

Export Citation Format

Share Document