scholarly journals Binding of sorbitol 6-phosphate and of fructose 1-phosphate to the regulatory protein of liver glucokinase

1992 ◽  
Vol 286 (1) ◽  
pp. 253-256 ◽  
Author(s):  
A Vandercammen ◽  
M Detheux ◽  
E Van Schaftingen
Keyword(s):  
Ethylene Glycol ◽  
Conformational Changes ◽  
Binding Assay ◽  
Regulatory Protein ◽  
Free Ligand ◽  
Phosphate Esters ◽  
Poly Ethylene Glycol ◽  
Apparent Affinity ◽  
Poly Ethylene ◽  
Scatchard Plots

Using a binding assay in which the ligand-protein complex is separated from free ligand by precipitation with poly(ethylene glycol) 6000, we found that the regulatory protein of rat liver glucokinase bound close to 1 mol of radiolabelled sorbitol 6-phosphate, a negative effector, or of fructose 1-phosphate, a positive effector, per mol of regulatory protein. Scatchard plots were linear, the dissociation constant being 0.3 microM for both phosphate esters. Sorbitol 6-phosphate and fructose 1-phosphate competed with each other for the binding. Competition was also observed with psicose 1-phosphate, ribitol 5-phosphate, arabitol 5-phosphate and 3-phosphoglycerate, all of which are known to affect the inhibition exerted by the regulatory protein. At a concentration of 10%, poly(ethylene glycol) 6000 decreased the concentration of regulatory protein causing 50% inhibition to a larger extent in the absence (12-fold) than in the presence (3-fold) of a saturating concentration of fructose 6-phosphate, another negative effector. Furthermore, it increased by about 3-fold the apparent affinity for inhibitory phosphate esters, indicating that it induced conformational changes of the regulatory protein.

CD47 Functionalization of Nanoparticles as a Poly(ethylene glycol) Alternative: A Novel Approach to Improve Drug Delivery

2021 ◽  
Vol 22 ◽  
Author(s):  
Noushin Rezaei Vandchali ◽  
Fatemeh Moadab ◽  
Eskandar Taghizadeh ◽  
Amir Tajbakhsh ◽  
Seyed Mohammad Gheibi-hayat
Keyword(s):  
Drug Delivery ◽  
Ethylene Glycol ◽  
Retention Time ◽  
Blood Circulation ◽  
Regulatory Protein ◽  
Reticuloendothelial System ◽  
Poly Ethylene Glycol ◽  
Drug Delivery Vehicles ◽  
Novel Approach ◽  
Poly Ethylene

Abstract: Bio-degradable nanoparticles (NPs) have several utilizations as the drug delivery vehicles due to their acceptable bio-availability, lower toxicity, potency for encapsulation and controlled release. Moreover, interaction of the NPs with the macrophages of reticuloendothelial system (RES) may decrease NPs efficacy for medical purposes. The surface of NPs is conventionally neutralized with the molecules such as poly(ethylene glycol) (PEG), as one of the most widely applied stealth polymers, in order to restrict the NPs clearance through the RES system. In fact, these molecules exhibit resistance to the RES clearance and proteins adsorption. It is unfortunate that modifying the PEG has some shortcomings like problems in the synthesis as well as correlation to the immune reaction. The CD47 receptor has been well known as a ‘don’t-eat-me’ molecule on the self-cells' surface. Therefore, the receptor will inhibit phagocytosis via binding to its ligand that is known as the signal regulatory protein α (SIRP-α). Moreover, the CD47 receptor, as one of the biomimetic substances, or its derivative peptides have been used recently on the surface of nanoparticles to inhibit phagocytosis and increase the NPs retention time in the blood circulation. Therefore, this review study examined the CD47 receptor and its role in the immune system as well as the use of the CD47 receptor in coating NPs to increase their retention time in the blood circulation.

Thermoresponsive Self-assembly and Conformational Changes of Amphiphilic Monodisperse Short Poly(ethylene glycol)s in Water

2014 ◽  
Vol 43 (7) ◽  
pp. 1055-1057 ◽  
Author(s):  
Nabanita Sadhukhan ◽  
Takahiro Muraoka ◽  
Daisuke Abe ◽  
Yuji Sasanuma ◽  
Dwiky Rendra Graha Subekti ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Conformational Changes ◽  
Self Assembly ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Mannosylated brush copolymers based on poly(ethylene glycol) and poly(ε-caprolactone) as multivalent lectin-binding nanomaterials

2019 ◽  
Vol 10 ◽  
pp. 2192-2206 ◽  
Author(s):  
Stefania Ordanini ◽  
Wanda Celentano ◽  
Anna Bernardi ◽  
Francesco Cellesi
Keyword(s):  
Molecular Weight ◽  
Ethylene Glycol ◽  
Ring Opening ◽  
Binding Assay ◽  
Lectin Binding ◽  
Aqueous Media ◽  
Poly Ethylene Glycol ◽  
High Control ◽  
Poly Ethylene ◽  
Macromolecular Architecture

A class of linear and four-arm mannosylated brush copolymers based on poly(ethylene glycol) and poly(ε-caprolactone) is presented here. The synthesis through ring-opening and atom transfer radical polymerizations provided high control over molecular weight and functionality. A post-polymerization azide–alkyne cycloaddition allowed for the formation of glycopolymers with different mannose valencies (1, 2, 4, and 8). In aqueous media, these macromolecules formed nanoparticles that were able to bind lectins, as investigated by concanavalin A binding assay. The results indicate that carbohydrate–lectin interactions can be tuned by the macromolecular architecture and functionality, hence the importance of these macromolecular properties in the design of targeted anti-pathogenic nanomaterials.

PIEZOELECTRIC PROPERTIES OF POLY(3-HYDROXYBUTYRATE-CO-3-HEDROXYBUTYRATE)-CO-POLY(ETHYLENE GLYCOL) PRODUCED BY CONTROLLED MICROBIOLOGICAL BIOSYNTHESIS

2019 ◽  
Vol 10 (10) ◽  
Author(s):  
Anton Bonartsev ◽  
Vera Voinova ◽  
Elizaveta Akoulina ◽  
Andrey Dudun ◽  
Irina Zharkova ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Piezoelectric Properties ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Thermosensitives hydrogels based on poly (ethylene glycol): I. Synthesis, characterization and release

2007 ◽  
Vol 32 (5) ◽  
pp. 431-446 ◽  
Author(s):  
Tahar Bartil ◽  
Mahmoud Bounekhel ◽  
Cedric Calberg ◽  
Robert Jerome
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene
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