scholarly journals Structural characterization of a novel glycosyl-phosphatidylinositol from the protozoan Tetrahymena mimbres

1991 ◽  
Vol 279 (2) ◽  
pp. 605-608 ◽  
Author(s):  
U Weinhart ◽  
J R Thomas ◽  
Y Pak ◽  
G A Thompson ◽  
M A J Ferguson
Keyword(s):  
Phospholipase C ◽  
Structural Characterization ◽  
Protozoan Parasites ◽  
Free Living ◽  
Glycosyl Phosphatidylinositol

A glycolipid metabolically labelled with [14C]GlcN was isolated from the free-living protozoan Tetrahymena mimbres. The glycolipid was sensitive to a bacterial phosphatidylinositol-specific phospholipase C, and the headgroup was shown to contain a phosphorylated Man alpha 1-2Man alpha 1-4Man alpha 1-4GlcN glycan. The Tetrahymena glycolipid is structurally unique among the glycosylphosphatidylinositols that have so far been characterized, including those from several protozoan parasites of humans.

scholarly journals Characterization of antibodies to the glycosyl-phosphatidylinositol membrane anchors of mammalian proteins

1991 ◽  
Vol 273 (2) ◽  
pp. 301-301 ◽  
Author(s):  
N M Hooper ◽  
S J Broomfield ◽  
A J Turner
Keyword(s):  
Phospholipase C ◽  
Cross Reactivity ◽  
Side Chain ◽  
Glycosyl Phosphatidylinositol ◽  
Anchor Structure ◽  
Polyclonal Antisera ◽  
Aminopeptidase P ◽  
Species Specific ◽  
Mild Acid

Two polyclonal antisera were raised in rabbits to the phospholipase C-solubilized forms of pig renal dipeptidase (EC 3.4.13.11) and pig aminopeptidase P (EC 3.4.11.9). These antisera were purified and shown to cross-react with other glycosyl-phosphatidylinositol (G-PI)-anchored proteins isolated from pig, human and trypanosomes. The epitopes involved in this cross-reactivity were characterized by Western-blot analysis after mild acid or nitrous acid treatment of the G-PI-anchored proteins and by a competitive e.l.i.s.a. with other G-PI-anchored proteins and individual components of the anchor structure. These studies revealed that the primary epitope for both antisera is the inositol 1.2-(cyclic)monophosphate that is formed on phospholipase C cleavage of the intact G-PI anchor. Other minor epitopes, such as phosphoethanolamine, probably involve side-chain modifications to the core anchor structure that may be species-specific.

scholarly journals The Molecular and Structural Characterization of Two Vitellogenins from the Free-Living Nematode Oscheius tipulae

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53460 ◽  
Author(s):  
Daniela P. Almenara ◽  
Joselene P. de Moura ◽  
Cristiane P. Scarabotto ◽  
Russolina B. Zingali ◽  
Carlos E. Winter
Keyword(s):  
Structural Characterization ◽  
Free Living ◽  
Free Living Nematode

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

Structural characterization of the PPIase domain of FKBP51, a cochaperone of human Hsp90

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
A Bracher ◽  
C Kozany ◽  
AK Thost ◽  
F Hausch
Keyword(s):  
Structural Characterization ◽  
Ppiase Domain

Structural characterization of dendrobine-type sesquiterpene alkaloids from the stems of Dendrobium nobile using LC-MS/MS

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
YH Wang ◽  
B Avula ◽  
N Abe ◽  
F Wei ◽  
M Wang ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Dendrobium Nobile
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