scholarly journals Structural and functional studies on the human hepatic interleukin-6 receptor. Molecular cloning and overexpression in HepG2 cells

1991 ◽  
Vol 277 (3) ◽  
pp. 659-664 ◽  
Author(s):  
H Schooltink ◽  
T Stoyan ◽  
D Lenz ◽  
H Schmitz ◽  
T Hirano ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Hepg2 Cells ◽  
Polyclonal Antiserum ◽  
Cross Linking ◽  
Human Hepatoma Cells ◽  
Functional Studies ◽  
Low Concentrations ◽  
Interleukin 6 Receptor ◽  
Cloned Cdna ◽  
The One

cDNAs coding for the human hepatic interleukin-6 receptor (IL-6-R) have been isolated from a library made from poly(A) RNA of dexamethasone-treated human hepatoma cells (HepG2). We found the hepatic IL-6-R to be identical to the one expressed by leucocytes. A polyclonal antiserum was raised in rabbits against the IL-6-R protein expressed in Escherichia coli. Although the entire IL-6-R protein was used for immunization, only antibodies to the cytoplasmic domain of the IL-6-R were obtained. It is demonstrated by affinity cross-linking and subsequent immunoprecipitation with antibodies against the ligand as well as against the receptor that the cloned cDNA codes for the functional IL-6-R on HepG2 cells. When the hepatic IL-6-R cDNA was overexpressed in HepG2 cells, these cells became more sensitive to low concentrations of IL-6 with respect to the induction of gamma-fibrinogen mRNA.

scholarly journals The H1 and H2 polypeptides associate to form the asialoglycoprotein receptor in human hepatoma cells.

1988 ◽  
Vol 106 (4) ◽  
pp. 1067-1074 ◽  
Author(s):  
J Bischoff ◽  
S Libresco ◽  
M A Shia ◽  
H F Lodish
Keyword(s):  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Asialoglycoprotein Receptor ◽  
Cross Linking ◽  
Human Hepatoma Cells ◽  
Cell Monolayers ◽  
Control Serum ◽  
Microsomal Membranes ◽  
Minimum Estimate ◽  
Chemical Cross Linking

Antibody-induced degradation and chemical cross-linking experiments have been carried out to assess the nature of the interaction between the two asialoglycoprotein-receptor polypeptides, H1 and H2, synthesized in HepG2 cells. Incubation of HepG2 cell monolayers with anti-H1 antibody caused a specific and equal loss of both H1 and H2 polypeptides. The same result was obtained with anti-H2 antibody. Control serum did not affect the level of H1 or H2 not did anti-H1 or anti-H2 antibodies affect the level of the transferrin receptor. The chemical cross-linking reagent, difluorodinitrobenzene, has been used to demonstrate that H1 can be cross-linked to H2 in HepG2 cell microsomal membranes. Dimer and trimer species with apparent molecular masses of 93 and 148 kD, respectively, were readily observed upon chemical cross-linking and some dimers and trimers were immunoreactive with both anti-H1 and anti-H2 antibodies. The putative trimer, possibly two H1 and one H2 molecules, is a minimum estimate of the true size of the asialoglycoprotein receptor in intact HepG2 cell, and it is possible that larger hetero-oligomeric forms of the receptor exist. The results of both types of experiments indicate that H1 and H2 form an oligomeric complex in HepG2 cells and thus, both polypeptides constitute the human asialoglycoprotein receptor.

scholarly journals Soluble human interleukin-6-receptor modulates interleukin-6-dependentN-glycosylation of α1-protease inhibitor secreted by HepG2 cells

FEBS Letters ◽  
1992 ◽  
Vol 306 (2-3) ◽  
pp. 257-261 ◽  
Author(s):  
Andrzej Mackiewicz ◽  
Stefan Rose-John ◽  
Heidi Schooltink ◽  
Maria Laciak ◽  
Aleksander Górny ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Interleukin 6 ◽  
Hepg2 Cells ◽  
Interleukin 6 Receptor

The effect of tocilizumab - an interleukin-6 receptor antagonist - on lipid levels in rheumatoid arthritis

2017 ◽  
Author(s):  
Ifigenia Kostoglou-Athanassiou ◽  
Lambros Athanassiou ◽  
Aikaterini Tzanavari ◽  
Charoula Katsavouni ◽  
Markos Kostopoulos ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Receptor Antagonist ◽  
Interleukin 6 ◽  
Lipid Levels ◽  
Interleukin 6 Receptor

A synthetic corticosteroid, dexamethasone regulates generation of soluble form of interleukin-6 receptor of human lymphocytes, in vitro

2002 ◽  
Vol 53 (3) ◽  
pp. 307-315 ◽  
Author(s):  
Anna Polgár ◽  
Márta Brózik ◽  
Sára Tóth ◽  
Marcsilla Holub ◽  
A. Falus
Keyword(s):  
Interleukin 6 ◽  
Human Lymphocytes ◽  
Soluble Form ◽  
Interleukin 6 Receptor
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