scholarly journals The Id gene is activated by serum but is not required for de-differentiation in rat vascular smooth muscle cells

1991 ◽  
Vol 277 (1) ◽  
pp. 285-288 ◽  
Author(s):  
P R Kemp ◽  
D J Grainger ◽  
C M Shanahan ◽  
P L Weissberg ◽  
J C Metcalfe
Keyword(s):  
Cell Cycle ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Myosin Heavy Chain ◽  
Vascular Smooth Muscle Cells ◽  
Heavy Chain ◽  
Muscle Cells ◽  
G1 Phase ◽  
Cells Cultured

Primary rat vascular smooth muscle cells cultured on fibronectin in the absence of serum lost smooth-muscle-specific myosin heavy chain but did not enter the cell cycle and proliferate until they were stimulated by serum. Under these conditions accumulation of Id mRNA occurred only in response to serum and was maximal during the G1 phase of the cycle.

Molecular cloning of a diverged homeobox gene that is rapidly down-regulated during the G0/G1 transition in vascular smooth muscle cells

1993 ◽  
Vol 13 (6) ◽  
pp. 3722-3733
Author(s):  
D H Gorski ◽  
D F LePage ◽  
C V Patel ◽  
N G Copeland ◽  
N A Jenkins ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Smooth Muscle ◽  
Growth Factor ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Molecular Cloning ◽  
Vascular Smooth Muscle Cells ◽  
Homeobox Gene ◽  
Muscle Cells ◽  
Regulatory Function

Adult vascular smooth muscle cells dedifferentiate and reenter the cell cycle in response to growth factor stimulation. Here we describe the molecular cloning from vascular smooth muscle, the structure, and the chromosomal location of a diverged homeobox gene, Gax, whose expression is largely confined to the cardiovascular tissues of the adult. In quiescent adult rat vascular smooth muscle cells, Gax mRNA levels are down-regulated as much as 15-fold within 2 h when these cells are induced to proliferate with platelet-derived growth factor (PDGF) or serum growth factors. This reduction in Gax mRNA is transient, with levels beginning to rise between 8 and 24 h after mitogen stimulation and returning to near normal by 24 to 48 h. The Gax down-regulation is dose dependent and can be correlated with the mitogen's ability to stimulate DNA synthesis. PDGF-AA, a weak mitogen for rat vascular smooth muscle cells, did not affect Gax transcript levels, while PDGF-AB and -BB, potent mitogens for these cells, were nearly as effective as fetal bovine serum. The removal of serum from growing cells induced Gax expression fivefold within 24 h. These data suggest that Gax is likely to have a regulatory function in the G0-to-G1 transition of the cell cycle in vascular smooth muscle cells.

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