scholarly journals Amino acid sequences of Euglena viridis ferredoxin and cytochromes c

1991 ◽  
Vol 276 (1) ◽  
pp. 47-52 ◽  
Author(s):  
R P Ambler ◽  
M D Kamen ◽  
R G Bartsch ◽  
T E Meyer
Keyword(s):  
Single Species ◽  
Amino Acid Sequences ◽  
British Library ◽  
Sequence Determination ◽  
Genetic Studies ◽  
Sequence Identity ◽  
Cytochromes C ◽  
Characteristic Features ◽  
Almost All ◽  
Sequence Identities

The Order Euglenida comprises many species and perhaps 40 genera, but almost all biochemical and genetic studies have been limited to a single species. Euglena gracilis, because of its ease of growth in the laboratory. Sequence studies of chloroplast and mitochondrial proteins from E. gracilis show that they have diverged widely from other eukaryotic lines. In the present paper we report the sequences of three proteins from another euglenoid, Euglena viridis, using material isolated from a natural bloom. The mitochondrial cytochrome c shows more than 90% sequence identity with that from E. gracilis, and contains the same characteristic features. The chloroplast cytochrome c6 has diverged to a greater extent and shows only 77% identity. The chloroplast ferredoxin from E. viridis is similar in sequence to those of cyanobacteria and algal chloroplasts, with sequence identities of up to 75%. Details of the purification, analysis and sequence determination experiments on the peptides have been deposited as Supplementary Publication SUP 50163 (32 pages) at the British Library Document Supply Centre, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1991) 273, 5.

scholarly journals Three neurotoxins from the venom of a sea snake Astrotia stokesii, including two long-chain neurotoxic proteins with amidated C-termini

1978 ◽  
Vol 175 (2) ◽  
pp. 507-517 ◽  
Author(s):  
N Maeda ◽  
N Tamiya
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequences ◽  
Amide Group ◽  
British Library ◽  
Amino Acid Residues ◽  
Snake Venoms ◽  
Sea Snake ◽  
X Ray ◽  
Almost All ◽  
Long Chain

From the venom of a sea snake Astrotia stokesii three neurotoxic components, toxins Astrotia stokesii a, b and c were isolated in 40, 15 and 5% yield by weight respectively of the whole venom. Their LD50 values for 20g mice were 0.13, 0.096 and 0.098 microgram/g body wt. respectively and accounted for almost all the lethal activity of the venom. Their amino acid sequences were determined. Astrotia stokesii a was composed of 60 amino acid residues with nine half-cystine residues and was quite homologous to other sea-snake short-chain neurotoxins in its amino acid sequence. Toxins Astrotia stokesii b and c were composed of 70 and 72 amino acid residues respectively with 10 half-cystine residues. They are the first long-chain neurotoxins with high activity isolated from sea-snake venoms. The C-terminal carboxy groups of toxins b and c were found to be amidated; the amidation is known for some polypeptides, but is novel for a protein. The amide group may make a hydrogen-bond with glutamic acid-39, which replaces a lysine that has so far been found invariably in long-chain neutrotoxins. Astrotia stokesii b and c are also novel in having phenylalanine-25 and isoleucine- or valine-42. The ordinary Tyr-Glu pair, which is observed in X-ray structure [Low, Preston, Sato, Rosen, Searl, Rudko & Richardson (1976) Proc. Natl. Acad. Sci. U.S.A. 73, 2991-2994] and n.m.r.study [Inagaki, Tatsumi, Miyazawa, Hori & Tamiya (1977) Abstr. Int. Congr. Pure Appl. Chem. 26th, p. 336] on erabutoxins may be replaced by a hydrophobic pair. Detailed evidence for the amino acid sequences of the proteins has been deposited as Supplementary Publication SUP 5009o (30 pages) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7B1, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1978) 169, 5.

scholarly journals The amino acid sequences of the cytochromes c-555 from two green sulphur bacteria of the genus Chlorobium

1976 ◽  
Vol 159 (3) ◽  
pp. 757-769 ◽  
Author(s):  
J Van Beeumen ◽  
R P Ambler ◽  
T E Meyer ◽  
M D Kamen ◽  
J M Olson ◽  
...  
Keyword(s):  
Amino Acid ◽  
Polypeptide Chain ◽  
Amino Acid Sequences ◽  
British Library ◽  
Comparative Biochemistry ◽  
Chlorobium Limicola ◽  
Cytochromes C ◽  
Sulphur Bacteria ◽  
Green Sulphur ◽  
Further Development

Amino acid seauences are proposed for the cytochromes c-555 from Chlorobium thiosulphatophilum and from the Chlorobium limicola component of “Chloropseudomonas ethylica 2K”. Each is a sincle polypeptide chain, the former of 86, the latter of 99 residues, and, when aligned so as to give the best match, 47 residues are common to the two sequences. The sequences show some resemblance to those of cytochromes c5 and f. The bacteriochlorophyll a-proteins were also isolated and purified, and their amino acid compositions compared (see the Appendix). There are significant differences in the compositions, but not as great as those found for the cytochromes c-555. The significance of these observations for the taxonomy of the Chlorobiaceae and for the further development of the comparative biochemistry of cytochrome c is discussed. Detailed evidence for the sequences of the cytochromes c-555 has been deposited as Supplementary Publication SUP 50073 (36 pages) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1976) 153, 5.

scholarly journals The amino acid sequence of cytochromes c-551 from three species of Pseudomonas

1973 ◽  
Vol 131 (3) ◽  
pp. 485-498 ◽  
Author(s):  
R. P. Ambler ◽  
Margaret Wynn
Keyword(s):  
Amino Acids ◽  
Pseudomonas Aeruginosa ◽  
Amino Acid ◽  
Cytochrome C ◽  
Amino Acid Sequence ◽  
Amino Acid Sequences ◽  
Mitochondrial Cytochrome ◽  
Cytochromes C ◽  
Lending Library ◽  
Single Peptide

The amino acid sequences of the cytochromes c-551 from three species of Pseudomonas have been determined. Each resembles the protein from Pseudomonas strain P6009 (now known to be Pseudomonas aeruginosa, not Pseudomonas fluorescens) in containing 82 amino acids in a single peptide chain, with a haem group covalently attached to cysteine residues 12 and 15. In all four sequences 43 residues are identical. Although by bacteriological criteria the organisms are closely related, the differences between pairs of sequences range from 22% to 39%. These values should be compared with the differences in the sequence of mitochondrial cytochrome c between mammals and amphibians (about 18%) or between mammals and insects (about 33%). Detailed evidence for the amino acid sequences of the proteins has been deposited as Supplementary Publication SUP 50015 at the National Lending Library for Science and Technology, Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1973), 131, 5.

Cranial morphology and systematics of an extraordinary sample of the Late Neogene dwarf tapir, Tapirus polkensis (Olsen)

2009 ◽  
Vol 83 (2) ◽  
pp. 238-262 ◽  
Author(s):  
Richard C. Hulbert ◽  
Steven C. Wallace ◽  
Walter E. Klippel ◽  
Paul W. Parmalee
Keyword(s):  
Single Species ◽  
Relative Strength ◽  
Cranial Morphology ◽  
Linear Measurements ◽  
Average Mass ◽  
Gray Fossil Site ◽  
Extant Species ◽  
Late Neogene ◽  
Fossil Site ◽  
Almost All

The previously poorly known “Tapiravus” polkensis Olsen, 1960 (Mammalia, Perissodactyla, Tapiridae) is now known from abundant, well preserved specimens from both the type area in central Florida and from the Gray Fossil Site (GFS) in eastern Tennessee. The latter has produced over 75 individuals, the greatest number of tapirids from a single fossil site, including many articulated skeletons. Almost all linear measurements taken on skulls, mandibles, and cheek teeth from GFS have coefficients of variation less than 10 (most between 3 and 7), indicating the presence of a single species. However, the sample reveals considerable intraspecific variation for a few key morphologic features, including development of the sagittal crest, outline shape of the nasals, and number and relative strength of lingual cusps on the P1. The Florida sample of T. polkensis is more limited, but has the same state as the GFS sample for all preserved characters of systematic significance, and while the Florida teeth are on average smaller (especially narrower lower cheek teeth), they fall either within or just below the observed range of the Gray Fossil Site population. The new material supports a reassignment of “Tapiravus” polkensis to the genus Tapirus, and demonstrates that the geologic age of the species is significantly younger than previously thought, Hemphillian rather than Barstovian. Tapirus polkensis is the smallest known North American Tapirus, and smaller than any of the extant species in the genus, with an estimated average mass of 125 kg.

scholarly journals Ultrasound Diagnostics of the Nature and Duration of Abdominal Trauma

2021 ◽  
Vol 6 (2) ◽  
pp. 169-180
Author(s):  
O. P. Babkina ◽  
◽  
S. I. Danylchenko ◽  
Keyword(s):  
Alcohol Intoxication ◽  
Abdominal Cavity ◽  
Kidney Damage ◽  
Mechanical Trauma ◽  
Free Fluid ◽  
Liver Size ◽  
Ultrasound Diagnostics ◽  
Characteristic Features ◽  
Size Heterogeneity ◽  
Almost All

The purpose of the study was to study a complex of changes in ultrasound parameters of injured tissues of the liver, spleen, pancreas in mechanical trauma and their dependence on the nature and duration of injury. Materials and methods. Ultrasound examinations were performed in 60 injured patients (41 males and 19 females) aged 20 to 60 years old in the dynamics as for the 1st, 2nd, 3rd, 4th and 5th days after injury. Puncture, transverse and oblique scans from all possible accesses were used. The conditions of the liver, pancreas, spleen, kidneys were studied. Results and discussion. In the course of the studies, a high number of injuries to the abdominal and retroperitoneal organs was demonstrated; it was proved that isolated liver injuries were observed in 32%, in 68% the liver injury was combined with injuries of other abdominal organs. We observed isolated damage to the pancreas in 10%, in the remaining 90%, the injury to the pancreas was combined with damage to the liver, gallbladder and extrahepatic bile ducts, spleen, and intestines. Isolated injuries of the spleen were observed in 31% of patients; in other cases, the injury to the spleen was combined with injuries of other organs of the abdominal cavity. We observed isolated kidney damage in almost 15%. In 85% of cases, kidney damage was observed in combination with injuries of other parenchymal organs. It has been demonstrated that in the area of injured tissues of the liver, pancreas, spleen, and kidneys with blunt trauma to the abdomen in persons with no signs of alcohol intoxication, regular ultrasound changes are observed, which are in direct proportion to the time elapsed since the injury was caused. The main ultrasound signs of liver damage were ruptures of the parenchyma with the formation of intra-parenchymal or subcapsular hematomas. In general, for almost all the injured people the following sings were characteristic as an increase in liver size (often due to increased part of damage), heterogeneity of structure, blurred contours and their discontinuity, presence of hypo- and anechoic areas (hematomas) on the background of intact parenchyma. Characteristic ultrasound criteria of pancreatic damage in the first day are the lack of clear contours of the gland, their discontinuity, increase in the size of the gland, the presence of hypoechoic areas of different sizes. At ultrasound examination of the spleen, the main signs of its damage are the heterogeneity of the parenchyma and the presence of anechoic structure and free fluid in the abdominal cavity. In 69% of cases, the main sings were blurred and uneven contours of the spleen, as well as an increase in its size. Characteristic features of renal hematoma are an increase in their size, heterogeneity of structure with increasing echogenicity of the parenchyma, blurred contours and their discontinuity. Conclusion. It has been proven that it is advisable to use in a complex of changes in the parenchymal organs revealed by ultrasound examinations, both in cases of isolated organ trauma, and in cases of combined trauma of the abdominal cavity and retroperitoneal organs, to determine the time of causing damage, since it makes it possible to increase the objectivity of the results of establishing the duration of the injury

scholarly journals Insertions and deletions trigger adaptive walks in Drosophila proteins

2012 ◽  
Vol 279 (1740) ◽  
pp. 3075-3082 ◽  
Author(s):  
Evgeny V. Leushkin ◽  
Georgii A. Bazykin ◽  
Alexey S. Kondrashov
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequences ◽  
Molecular Adaptation ◽  
Amino Acid Substitutions ◽  
Protein Coding ◽  
Evolution Of Life ◽  
High Fraction ◽  
Adaptive Walks ◽  
The Difference ◽  
Almost All

Maps that relate all possible genotypes or phenotypes to fitness—fitness landscapes—are central to the evolution of life, but remain poorly known. An insertion or a deletion (indel) of one or several amino acids constitutes a substantial leap of a protein within the space of amino acid sequences, and it is unlikely that after such a leap the new sequence corresponds precisely to a fitness peak. Thus, one can expect an indel in the protein-coding sequence that gets fixed in a population to be followed by some number of adaptive amino acid substitutions, which move the new sequence towards a nearby fitness peak. Here, we study substitutions that occur after a frame-preserving indel in evolving proteins of Drosophila . An insertion triggers 1.03 ± 0.75 amino acid substitutions within the protein region centred at the site of insertion, and a deletion triggers 4.77 ± 1.03 substitutions within such a region. The difference between these values is probably owing to a higher fraction of effectively neutral insertions. Almost all of the triggered amino acid substitutions can be attributed to positive selection, and most of them occur relatively soon after the triggering indel and take place upstream of its site. A high fraction of substitutions that follow an indel occur at previously conserved sites, suggesting that an indel substantially changes selection that shapes the protein region around it. Thus, an indel is often followed by an adaptive walk of length that is in agreement with the theory of molecular adaptation.

scholarly journals Protein-sequence studies on Rh-related polypeptides suggest the presence of at least two groups of proteins which associate in the human red-cell membrane

1988 ◽  
Vol 256 (3) ◽  
pp. 1043-1046 ◽  
Author(s):  
N D Avent ◽  
K Ridgwell ◽  
W J Mawby ◽  
M J Tanner ◽  
D J Anstee ◽  
...  
Keyword(s):  
Amino Acid ◽  
Blood Group ◽  
Protein Sequence ◽  
Amino Acid Sequences ◽  
Red Cells ◽  
British Library ◽  
Blood Group System ◽  
Terminal Amino Acid ◽  
Red Cell Membrane ◽  
Terminal Amino

The Rh D blood-group antigen forms part of a complex, involving several other polypeptides, that is deficient in the red cells of individuals who lack all the antigens of the Rh blood-group system (Rhnull red cells). These include components recognized by anti-(Rh D) antibodies and the murine monoclonal antibodies R6A and BRIC 125. We have carried out protein-sequence studies on the components immunoprecipitated by these antibodies. Anti-(Rh D) antibodies immunoprecipitate an Mr-30,000-32,000 polypeptide (the D30 polypeptide) and an Mr-45,000-100,000 glycoprotein (D50 polypeptide). Antibody R6A immunoprecipitates two glycoproteins of Mr 31,000-34,000 (R6A32 polypeptide) and Mr 35,000-52,000 (R6A45 polypeptide). The D30 and R6A32 polypeptides were found to have the same N-terminal amino acid sequences, showing that they are closely related proteins. The D50 polypeptide and the R6A45 polypeptide also had indistinguishable N-terminal amino acid sequences that differed from that of the D30 and R6A32 polypeptides. The putative N-terminal membrane-spanning segments of the two groups of proteins showed homology in their amino acid sequence, which may account for the association of each of the pairs of proteins during co-precipitation by the antibodies. Supplementary data related to the protein sequence have been deposited as Supplementary Publication SUP 50417 (6 pages) at the British Library Document Supply Centre, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1988) 249, 5.

Abstract WP270: Etiopathologies of Symptomatic and Asymptomatic Intracranial Large Arterial Disease: The Results of HR-MRI and Genetic Studies

Stroke ◽  
2020 ◽  
Vol 51 (Suppl_1) ◽  
Author(s):  
Hyung Jun Kim ◽  
Oh Young Bang ◽  
In-Young Baek ◽  
Mee So Lee ◽  
Ingyeong Kim ◽  
...  
Keyword(s):  
Arterial Disease ◽  
Imaging Features ◽  
Clinical Genetic ◽  
Double Lumen ◽  
Genetic Studies ◽  
High Resolution Mri ◽  
Tandem Stenosis ◽  
Distal Internal Carotid Artery ◽  
Characteristic Features ◽  
A Prospective Study

Introduction: Intracranial large arterial disease (ILAD) is common, especially in Asians. We compared clinical, genetic, and imaging features between symptomatic and asymptomatic ILAD. Methods: We conducted a prospective study of patients with acute symptomatic ILAD and subjects with asymptomatic ILAD diagnosed during medical checkups. Patients who had steno-occlusive lesions on the middle cerebral artery and/or distal internal carotid artery underwent high-resolution MRI (HR-MRI) and RNF213 (p.Arg4810Lys variant) gene studies. We excluded patients who had proximal embolic sources in ipsilateral carotid and heart. A presumptive etiology of ILAD was made based on HR-MRI and RNF213 gene studies. Patients were classified into intracranial atherosclerosis (ICAS) when relevant plaques existed on HR-MRI; moyamoya disease (MMD) when patients had RNF213 variant and HR-MRI showed no plaque but characteristic features of MMD (negative remodeling and basal collaterals); and intracranial arterial dissection (ICAD) when pathognomic features (intimal flap/double lumen or mural hematoma) exist on HR-MRI. Results: Among 224 patients analyzed, 124 were symptomatic and 100 were asymptomatic. ICAS-type was more common in symptomatic ILAD than in asymptomatic ILAD (61.4% vs. 45.0%, p=0.009). The RNF213 variant was observed in one third of ICAS-type ILAD (37.0% in asymptomatic and 38.6% in symptomatic). Similarly, the proportion of MMD-type was similar between asymptomatic (33, 21.3%) and symptomatic (32, 19.0%) ILAD. Multivariate testing showed that symptomatic ILAD was associated with the presence of intracranial plaque on HR-MRI, regardless of the presence of the RNF213 variant, site of ILAD, and number of tandem stenosis. Conclusions: While various etiopathologies can cause ILAD, ICAS is an important pathology of symptomatic ILAD and should be target for medical management.

Characteristic of basic tools of mobile learning in informatics

2021 ◽  
pp. 9-13
Author(s):  
I. V. Izhdeneva
Keyword(s):  
Mobile Devices ◽  
Mobile Learning ◽  
Cognitive Activity ◽  
Point Of View ◽  
Learning Tools ◽  
Difficult Situation ◽  
Characteristic Features ◽  
Definition Of ◽  
Almost All ◽  
Educational Potential

The article actualizes the possibilities of mobile learning based on the use of mobile devices and technologies available to almost all students. Various approaches to the definition of the concept of "mobile learning" are analyzed, including techno-centric and information-centric approaches. The concept of mobility as one of the attributes of learning tools is revealed. The importance of mobile learning in the current difficult situation of distance learning during the pandemic is substantiated. The characteristic features of mobile learning are highlighted, affecting its main functions, learning tools and independence from the time and place of learning. Possible directions for the implementation of mobile learning are characterized, from simple transfer of educational content to compliance with basic educational theories. From the point of view of the functioning and directions of development of mobile learning, its basic principles in teaching informatics are formulated. The types of students' activities are briefly characterized; some advantages of mobile learning are highlighted and its features as visualization, interactivity, efficiency of use in teaching children with disabilities are characterized. Some popular educational apps for mobile devices are considered, their characteristics are given, and their educational potential is revealed. Examples of mobile applications are given, the use of which in informatics lessons will help to activate the educational and cognitive activity of students and stimulate them to engage in informatics, coding and robotics.

scholarly journals Comparison of Prediction Equations for Estimating Inside Bark Diameters for Yellow-Poplar, Red Maple, and Red Pine in West Virginia

2009 ◽  
Vol 26 (1) ◽  
pp. 5-8 ◽  
Author(s):  
John R. Brooks ◽  
Lichun Jiang
Keyword(s):  
West Virginia ◽  
Single Species ◽  
Prediction Equation ◽  
Red Pine ◽  
Red Maple ◽  
Nonlinear Prediction ◽  
Prediction Equations ◽  
Yellow Poplar ◽  
Taper Function ◽  
Almost All

Abstract The ability to predict inside bark diameters was investigated using taper data for red maple, red pine and yellow-poplar from several stands in West Virginia. Inside bark estimates were based on Grosenbaugh's STX prediction equations, a segmented polynomial taper function fitted to inside bark diameter data, an existing polynomial prediction equation published for several hardwood species in this region and a nonlinear prediction equation fitted to the taper data. Grosenbaugh's STX is a computer program for processing tree measurements and includes three equations to allow flexibility and greater accuracy in predicting inside bark upper stem diameters. The nonlinear equation had the smallest overall prediction error in almost all cases investigated. The taper function had the largest error but does not require knowledge of the specific upper stem outside bark diameter. No single STX bark option was found to be optimal for all species or for a single species from two different geographic regions.

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