scholarly journals The reactive serine residue of epidermolytic toxin A

1990 ◽  
Vol 269 (2) ◽  
pp. 535-537 ◽  
Author(s):  
C J Bailey ◽  
T P Smith

Comparison of amino acid sequence data suggested that there may be a functional relationship between the staphylococcal epidermolytic toxins and V8 proteinase. The hypothesis was tested by treating epidermolytic toxin with di-isopropyl phosphorofluoridate, which bound specifically at serine-195, the homologue of the active-site serine residue of V8 proteinase.

1981 ◽  
Vol 154 (6) ◽  
pp. 1946-1956 ◽  
Author(s):  
M Pawlita ◽  
E Mushinski ◽  
R J Feldmann ◽  
M Potter

An IgG1 monoclonal antibody HyX24-14 was derived from A/J mice that were immunized with the IgA XRPC24 (X24) galactan binding myeloma protein (GalBMP) of BALB/c origin by the Kohler-Milstein hybridoma technology. HyX24-14 specifically binds some but all GalBMP. Different patterns of binding using a panel of nine Gal BMP were found, depending upon the concentration of antibody and the antigenic target. From molecular models and amino acid sequence data, ti was proposed that the idiotope defined by HyX24-14 had two subsites, each of which appeared to be able to bind independently to the antibody.


1981 ◽  
Vol 20 (7) ◽  
pp. 1559-1565 ◽  
Author(s):  
Cornelia Grund ◽  
John Gilroy ◽  
Tim Gleaves ◽  
Uwe Jensen ◽  
Donald Boulter

1986 ◽  
Vol 192 (4) ◽  
pp. 919-924 ◽  
Author(s):  
Monika Singhofer-Wowra ◽  
Melvyn Little ◽  
Lesley Clayton ◽  
Peter Dawson ◽  
Keith Gull

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