scholarly journals Co-ordinate expression of cytochrome c oxidase subunit III and VIc mRNAs in rat tissues

1990 ◽  
Vol 269 (2) ◽  
pp. 503-506 ◽  
Author(s):  
D A Hood
Keyword(s):  
Enzyme Activity ◽  
Steady State ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Mrna Level ◽  
Rat Tissues ◽  
Mrna Levels ◽  
Subunit Mrna ◽  
Oxidase Subunit ◽  
Subunit Iii

Cytochrome c oxidase (EC 1.9.3.1) is an enzyme which is composed of subunits derived from both the mitochondrial and the nuclear genomes. To determine whether or not the expression of these two genomes is co-ordinated at the mRNA level, we have examined the steady-state levels of mRNAs coding for cytochrome c oxidase subunit III (mitochondrially encoded) and subunit VIc (nuclear-encoded) in rat tissues. This was compared with the tissue concentration of the holoenzyme, which was estimated by measuring cytochrome c oxidase enzyme activity. The tissues (heart, brain, liver, kidney, soleus muscle and superficial white vastus muscle) possessed a 13-fold range of enzyme activity, which was highest in heart and lowest in the superficial vastus muscle. Specific subunit mRNA levels were quantified by using slot-blot hybridization of cDNA probes to total tissue RNA. The highest values for subunit III and Vlc mRNA tissue contents were found in kidney, followed by liver and heart (40-60% of that of kidney). The white vastus muscle contained the lowest subunit mRNA level (15% of that of kidney). Although some variability was apparent within each tissue, a parallel pattern of mRNA expression of the nuclear- and mitochondrially encoded subunits was observed. Differences between muscle (heart, vastus and soleus) and non-muscle tissues were noted in the relationship between mRNA and protein levels of expression. Thus, although this suggests that tissue-specific regulatory processes operate, the steady-state expression of subunit III and subunit Vlc mRNAs appears to be co-ordinately regulated.

Differential expression of cytochrome c oxidase subunit III gene in castes of the termite Reticulitermes santonensis

2006 ◽  
Vol 52 (6) ◽  
pp. 551-557 ◽  
Author(s):  
Marjorie A. Liénard ◽  
Jean-Marc X.S. Lassance ◽  
Ivan Paulmier ◽  
Jean-François Picimbon ◽  
Christer Löfstedt
Keyword(s):  
Cytochrome C ◽  
Differential Expression ◽  
Cytochrome C Oxidase ◽  
Oxidase Subunit ◽  
Reticulitermes Santonensis ◽  
Subunit Iii

Involvement of Cytochrome c Oxidase Subunit III in Energy Coupling

Biochemistry ◽  
1995 ◽  
Vol 34 (50) ◽  
pp. 16298-16305 ◽  
Author(s):  
Shaolong Wu ◽  
Rafael Moreno-Sanchez ◽  
Hagai Rottenberg
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Energy Coupling ◽  
Oxidase Subunit ◽  
Subunit Iii

scholarly journals Differential regulation of γ-glutamylcysteine synthetase heavy and light subunit gene expression

1997 ◽  
Vol 326 (1) ◽  
pp. 167-172 ◽  
Author(s):  
Jiaxin CAI ◽  
Zong-Zhi HUANG ◽  
Shelly C. LU
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Steady State ◽  
Mrna Level ◽  
Rat Hepatocytes ◽  
Mrna Levels ◽  
Subunit Gene ◽  
Subunit Mrna ◽  
Glutamylcysteine Synthetase ◽  
Steady State Mrna Level

γ-Glutamylcysteine synthetase (GCS) is the rate-limiting enzyme in the biosynthesis of glutathione and is composed of a heavy and a light subunit. Although the heavy subunit is enzymically active alone, the light subunit plays an important regulatory role by making the holoenzyme function more efficiently. In the current study we examined whether conditions which are known to influence gene expression of the heavy subunit also influence that of the light subunit, and the mechanisms involved. Treatment of cultured rat hepatocytes with hormones such as insulin and hydrocortisone, or plating hepatocytes under low cell density increased the steady-state mRNA level of the heavy subunit only. Treatment with diethyl maleate (DEM), buthionine sulphoximine (BSO) and t-butylhydroquinone (TBH) increased the steady state mRNA level and gene transcription rates of both subunits. These treatments share in common their ability to induce oxidative stress and activate nuclear factor κB (NF-κB). Treatment with protease inhibitors 7-amino-1-chloro-3-tosylamido-2-heptanone (TLCK) or L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK) had no influence on the basal NF-κB and GCS subunit mRNA levels, but blocked the activation of NF-κB by DEM, BSO and TBH, and the increase in GCS heavy subunit mRNA level by BSO and TBH. On the other hand, the DEM-, BSO- and TBH-induced increase in GCS light-subunit mRNA level was unaffected by TLCK and TPCK. Thus only the heavy subunit is hormonally regulated and growth sensitive, whereas both subunits are regulated by oxidative stress. Signalling through NF-κB is involved only in the oxidative-stress-mediated changes in the heavy subunit gene expression.

scholarly journals Cytochrome c oxidase subunit 4 isoform 2‐knockout mice show reduced enzyme activity, airway hyporeactivity, and lung pathology

2012 ◽  
Vol 26 (9) ◽  
pp. 3916-3930 ◽  
Author(s):  
Maik Hüttemann ◽  
Icksoo Lee ◽  
Xiufeng Gao ◽  
Petr Pecina ◽  
Alena Pecinova ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Knockout Mice ◽  
Lung Pathology ◽  
Oxidase Subunit

scholarly journals Isoforms of human cytochrome-c oxidase. Subunit composition and steady-state kinetic properties

1991 ◽  
Vol 199 (3) ◽  
pp. 615-622 ◽  
Author(s):  
Andre B. P. KUILENBURG ◽  
Henk L. DEKKER ◽  
Coby BOGERT ◽  
Popko NIEBOER ◽  
Bob F. GELDER ◽  
...  
Keyword(s):  
Steady State ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Subunit Composition ◽  
Kinetic Properties ◽  
Oxidase Subunit ◽  
Steady State Kinetic ◽  
Human Cytochrome ◽  
State Kinetic ◽  
Human Cytochrome C
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